Effects of stepwise changes in dissolved carbon dioxide concentrations on metabolic activity in Chlorella for spaceflight applications

Matula, Emily E.; Nabity, James

doi:10.1016/j.lssr.2021.03.005

Cited by 9 publications

(3 citation statements)

References 61 publications

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“…Likewise, these models can also be implemented in crop plants by exploiting conventional molecular biology tools as well as new genomeediting techniques. Besides crop plants, all our results are readily transferable to the highly conserved photorespiratory and photosynthetic cycles found in algae (Alami et al, 2021;Matula and Nabity, 2021), and blue-green algae (George et al, 2020). As algae and fast-growing land plants such as bamboo grass (Devi and Singh, 2021) are fast CO 2 harvesters even without engineering, they are often aimed for potential CO 2 -sequestration applications.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 56%

Topological Analysis of the Carbon-Concentrating CETCH Cycle and a Photorespiratory Bypass Reveals Boosted CO2-Sequestration by Plants

Osmanoğlu

AlSeiari

AlKhoori

et al. 2021

Front. Bioeng. Biotechnol.

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Synthetically designed alternative photorespiratory pathways increase the biomass of tobacco and rice plants. Likewise, some in planta–tested synthetic carbon-concentrating cycles (CCCs) hold promise to increase plant biomass while diminishing atmospheric carbon dioxide burden. Taking these individual contributions into account, we hypothesize that the integration of bypasses and CCCs will further increase plant productivity. To test this in silico, we reconstructed a metabolic model by integrating photorespiration and photosynthesis with the synthetically designed alternative pathway 3 (AP3) enzymes and transporters. We calculated fluxes of the native plant system and those of AP3 combined with the inhibition of the glycolate/glycerate transporter by using the YANAsquare package. The activity values corresponding to each enzyme in photosynthesis, photorespiration, and for synthetically designed alternative pathways were estimated. Next, we modeled the effect of the crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (CETCH), which is a set of natural and synthetically designed enzymes that fix CO₂ manifold more than the native Calvin–Benson–Bassham (CBB) cycle. We compared estimated fluxes across various pathways in the native model and under an introduced CETCH cycle. Moreover, we combined CETCH and AP3-w/plgg1RNAi, and calculated the fluxes. We anticipate higher carbon dioxide–harvesting potential in plants with an AP3 bypass and CETCH–AP3 combination. We discuss the in vivo implementation of these strategies for the improvement of C3 plants and in natural high carbon harvesters.

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Section: Conclusion and Future Prospectsmentioning

confidence: 56%

Topological Analysis of the Carbon-Concentrating CETCH Cycle and a Photorespiratory Bypass Reveals Boosted CO2-Sequestration by Plants

Osmanoğlu

AlSeiari

AlKhoori

et al. 2021

Front. Bioeng. Biotechnol.

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“…In addition, summer is the rainy season, with large rainfall and rapid water flow, which promotes the rise of DO concentration [36]. This provides favorable environmental conditions for the growth of algae, resulting in a significant increase in Chl-a concentration in water and serious deterioration of water quality [37].…”

Section: Spatial Heterogeneity Of Water Qualitymentioning

confidence: 99%

Spatio-Temporal Variations of Water Quality and Planktonic Algal Communities in Qingshan Reservoir, China

Yan¹,

Xu²,

Yang³

et al. 2023

Pol. J. Environ. Stud.

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Based on the monitoring data of conventional water quality in Qingshan Reservoir from 2018 to 2019, principal component analysis (PCA) and comprehensive nutritional status index (TLI) are used to evaluate water quality, planktonic algal communities, and eutrophication degree of Qingshan reservoir. The results showed that: 1) from 2018 to 2019, the annual average TLI is 56.54, indicating that the reservoir is in a slightly eutrophic state, which is conducive to algae growth; 2) the water quality of Qingshan Reservoir has obvious temporal and spatial variability: the water environment quality commonly decreased in the order winter>spring>autumn>summer, and exit zone>buffer zone>entry zone; meanwhile, the algal biomass in summer and autumn was significantly higher than that in spring and winter, and most of the algae were concentrated in the surface water except in January and decreased with the increase of depth; And related to the water quality environment, the biomass of cyanobacteria and green algae in Qingshan Reservoir is relatively high in summer, while diatom is the dominant species in spring and winter. We come to the conclusion that targeting at region and time will be more effective for the treatment of reservoir eutrophication.

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“…Algal photobioreactors can remove carbon dioxide (CO 2 ), liberate O 2 , remove or alter waste and produce edible biomass ( Matula and Nabity, 2019 ; Fahrion et al, 2021 ). These photobioreactors can withstand the dynamic temperature environment experienced within the ISS thermal control loops ( Matula and Nabity, 2021 ; Matula et al, 2021 ). Preliminary spaceflight studies using algae for ECLSS observed thriving cultures ( Helisch et al, 2020 ; Poughon et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Astrovirology: how viruses enhance our understanding of life in the Universe

Trubl

Stedman

Bywaters

et al. 2023

International Journal of Astrobiology

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Viruses are the most numerically abundant biological entities on Earth. As ubiquitous replicators of molecular information and agents of community change, viruses have potent effects on the life on Earth, and may play a critical role in human spaceflight, for life-detection missions to other planetary bodies and planetary protection. However, major knowledge gaps constrain our understanding of the Earth's virosphere: (1) the role viruses play in biogeochemical cycles, (2) the origin(s) of viruses and (3) the involvement of viruses in the evolution, distribution and persistence of life. As viruses are the only replicators that span all known types of nucleic acids, an expanded experimental and theoretical toolbox built for Earth's viruses will be pivotal for detecting and understanding life on Earth and beyond. Only by filling in these knowledge and technical gaps we will obtain an inclusive assessment of how to distinguish and detect life on other planetary surfaces. Meanwhile, space exploration requires life-support systems for the needs of humans, plants and their microbial inhabitants. Viral effects on microbes and plants are essential for Earth's biosphere and human health, but virus–host interactions in spaceflight are poorly understood. Viral relationships with their hosts respond to environmental changes in complex ways which are difficult to predict by extrapolating from Earth-based proxies. These relationships should be studied in space to fully understand how spaceflight will modulate viral impacts on human health and life-support systems, including microbiomes. In this review, we address key questions that must be examined to incorporate viruses into Earth system models, life-support systems and life detection. Tackling these questions will benefit our efforts to develop planetary protection protocols and further our understanding of viruses in astrobiology.

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Effects of stepwise changes in dissolved carbon dioxide concentrations on metabolic activity in Chlorella for spaceflight applications

Cited by 9 publications

References 61 publications

Topological Analysis of the Carbon-Concentrating CETCH Cycle and a Photorespiratory Bypass Reveals Boosted CO2-Sequestration by Plants

Topological Analysis of the Carbon-Concentrating CETCH Cycle and a Photorespiratory Bypass Reveals Boosted CO2-Sequestration by Plants

Spatio-Temporal Variations of Water Quality and Planktonic Algal Communities in Qingshan Reservoir, China

Astrovirology: how viruses enhance our understanding of life in the Universe

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