Effects of Storage and Granary Weevil Infestation on Gel Electrophoresis and Protein Solubility Properties of Hard and Soft Wheat Flours

Keskin, Şule; Yalçın, Erkan; Özkaya, Hazım

doi:10.1093/jee/toy041

Cited by 11 publications

(10 citation statements)

References 20 publications

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“…Previous reports also indicated that physiological properties in wheat can vary with the degree of hardness during accelerated ageing. Additionally, the post-harvest storage-tolerance of soft and hard wheat was different under the same storage conditions (Keskin et al, 2018;Zhang et al, 2017). Our current results showed that the Gr of soft wheat seeds of the 'Yangmai 15' cultivar was higher than that of hard wheat seeds of the 'Aikang 58' cultivar under the same storage conditions (Lv et al, 2016), confirming the superior post-harvest storage-tolerance of soft wheat.…”

Section: Manuscript To Be Reviewedsupporting

confidence: 56%

“…In wheat, kernel texture and biochemical composition vary with the degree of hardness (Lesage et al, 2011;Pasha et al, 2010). Previous researches showed that seed ageing causes the physiological and biochemical variation including differences in hardness during storage, and these changed characteristics have been extensively studied, specifically changes in antioxidants, free radicals, storage proteins, and respiration (Galleschi et al, 2002;Calucci et al, 2004;Keskin et al, 2018; PeerJ reviewing PDF | (2018:06:28953:1:0:NEW 1 Sep 2018)…”

Section: Introductionmentioning

confidence: 99%

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Peer Review #3 of "Dynamic proteomic changes in soft wheat seeds during accelerated ageing (v0.1)"

2018

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Previous research demonstrated that soft wheat cultivars have better post-harvest storage tolerance than harder cultivars during accelerated ageing. To better understand this phenomenon, a tandem mass tag-based quantitative proteomic analysis of soft wheat seeds was performed at different storage times during accelerated ageing (germination ratios of 97%, 45%, 28%, and 6%). A total of 1010 proteins were differentially regulated, of which 519 and 491 were up-and downregulated, respectively. Most of the differentially expressed proteins were predicted to be involved in nutrient reservoir, enzyme activity and regulation, energy and metabolism, and response to stimulus functions, consistent with processes occurring in hard wheat during artificial ageing. Notably, defenseassociated proteins including wheatwin-2, pathogenesis-related proteins protecting against fungal invasion, and glutathione S-transferase and glutathione synthetase participating in reactive oxygen species (ROS) detoxification, were upregulated compared to levels in hard wheat during accelerated ageing. These upregulated proteins might be responsible for the superior post-harvest storage-tolerance of soft wheat cultivars during accelerated ageing compared with hard wheat. Although accelerated ageing could not fully mimic natural ageing, our findings provided novel dynamic proteomic insight into soft wheat seeds during seed deterioration.

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Section: Manuscript To Be Reviewedsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Peer Review #3 of "Dynamic proteomic changes in soft wheat seeds during accelerated ageing (v0.1)"

2018

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“…[8,60,63,64]. Though some have found higher infestation and greater abundance of S. granarius on soft wheat compared with durum wheat cultivars [65,66], this could be because the main digestive enzyme for S. granarius is amylase [67]. Nawrot et al [60] found that seven times more amylase was present in S. granarius fed on soft wheat cultivars than on durum wheat cultivars.…”

Section: Discussionmentioning

confidence: 99%

Durum Wheat Cultivars Express Different Level of Resistance to Granary Weevil, Sitophilus granarius (Coleoptera; Curculionidae) Infestation

Lemić

Mikac

Genda

et al. 2020

Insects

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The granary weevil, Sitophilus granarius Linnaeus 1875, is a primary pest of stored grains worldwide. Feeding damage and progeny production of S. granarius was estimated to identify the levels of resistance of the insect on different durum wheat cultivars. Insect attack on four different durum wheat cultivars was investigated over a period of 20 weeks. Durum wheats were artificially infected with 20 individuals of S. granarius. Every two weeks the sample weight, hectoliter weight, moisture and the number of live weevils, including their number of progenies, were recorded. Overall findings revealed different levels of resistance of different durum wheat cultivars to S. granarius infestation. The Primadur cultivar had the highest resistance, followed by the Marco Aurelio and Cesare cultivars followed finally by the Tito Flavio cultivar which was highly susceptible to S. granarius. For all cultivars, apart from Primadur, S. granarius metabolism increased humidity and temperature, leading to grain degradation and resulting in the potential complete loss of market value if under field conditions. Evidently, durum wheat characteristics affect the life cycle of S. granarius, primarily their progeny, and thus the damage they undertake to the wheat itself. These findings are important because they enable the strategic selection of wheat cultivars that can be stored for a longer time period, while more sensitive wheat cultivars can be selected for shorter storage time and thus faster delivery to market.

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“…Previous reports also indicated that physiological properties in wheat can vary with the degree of hardness during accelerated ageing. Additionally, the post-harvest storage-tolerance of soft and hard wheat was different under the same storage conditions ( Keskin, Yalçın & Özkaya, 2018 ; Zhang et al, 2017 ). Our current results showed that the Gr of soft wheat seeds of the ‘Yangmai 15’ cultivar was higher than that of hard wheat seeds of the ‘Aikang 58’ cultivar under the same storage conditions ( Lv et al, 2016 ), confirming the superior post-harvest storage-tolerance of soft wheat.…”

Section: Discussionmentioning

confidence: 99%

“…In wheat, kernel texture and biochemical composition vary with the degree of hardness ( Lesage et al, 2011 ; Pasha, Anjum & Morris, 2010 ). Previous researches showed that seed ageing causes the physiological and biochemical variation including differences in hardness during storage, and these changed characteristics have been extensively studied, specifically changes in antioxidants, free radicals, storage proteins, and respiration ( Galleschi et al, 2002 ; Calucci et al, 2004 ; Keskin, Yalçın & Özkaya, 2018 ; McDonald, 1999 ; Zhang et al, 2017 ). Previous studies also indicate that the seed ageing effects the proteome of dry seeds ( Rajjou et al, 2008 ; Xin et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamic proteomic changes in soft wheat seeds during accelerated ageing

Tian

Zhang

et al. 2018

PeerJ

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Previous research demonstrated that soft wheat cultivars have better post-harvest storage tolerance than harder cultivars during accelerated ageing. To better understand this phenomenon, a tandem mass tag-based quantitative proteomic analysis of soft wheat seeds was performed at different storage times during accelerated ageing (germination ratios of 97%, 45%, 28%, and 6%). A total of 1,010 proteins were differentially regulated, of which 519 and 491 were up- and downregulated, respectively. Most of the differentially expressed proteins were predicted to be involved in nutrient reservoir, enzyme activity and regulation, energy and metabolism, and response to stimulus functions, consistent with processes occurring in hard wheat during artificial ageing. Notably, defense-associated proteins including wheatwin-2, pathogenesis-related proteins protecting against fungal invasion, and glutathione S-transferase and glutathione synthetase participating in reactive oxygen species (ROS) detoxification, were upregulated compared to levels in hard wheat during accelerated ageing. These upregulated proteins might be responsible for the superior post-harvest storage-tolerance of soft wheat cultivars during accelerated ageing compared with hard wheat. Although accelerated ageing could not fully mimic natural ageing, our findings provided novel dynamic proteomic insight into soft wheat seeds during seed deterioration.

show abstract

Effects of Storage and Granary Weevil Infestation on Gel Electrophoresis and Protein Solubility Properties of Hard and Soft Wheat Flours

Cited by 11 publications

References 20 publications

Peer Review #3 of "Dynamic proteomic changes in soft wheat seeds during accelerated ageing (v0.1)"

Peer Review #3 of "Dynamic proteomic changes in soft wheat seeds during accelerated ageing (v0.1)"

Durum Wheat Cultivars Express Different Level of Resistance to Granary Weevil, Sitophilus granarius (Coleoptera; Curculionidae) Infestation

Dynamic proteomic changes in soft wheat seeds during accelerated ageing

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