Effects of strain and embryo transfer model (embryos from one versus two donor does/recipient) on results of cryopreservation in rabbit

Vicente, J.S.; García-Ximénez, F.

doi:10.1051/rnd:19930101

Cited by 23 publications

(12 citation statements)

References 13 publications

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“…However, residual damage in surviving late blastocysts seems to still be present at the molecular level before the implantation process begins. In that sense, both the implantation and birth rates obtained were lower for frozen embryos, similar to findings in previous studies in rabbit [28][29][30][31][32]18]. Specifically in our work, from 29.9% of frozen embryos implanted, 84.1% were born alive from all recipient does, as is usual for rabbits (at approximately 20% of postimplantational losses [33][34][35]).…”

Section: Discussionsupporting

confidence: 91%

Effects of Slow Freezing Procedure on Late Blastocyst Gene Expression and Survival Rate in Rabbit1

Saenz-de-Juano

Marco‐Jiménez

Peñaranda

et al. 2012

Biology of Reproduction

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Studies of embryo cryopreservation efficiency have focused mainly on technical and embryo factors. To determine how a slow freezing process affects embryo and fetal development, we studied in vivo development ability after the freezing procedure by assessing blastocyst development at Day 6, implantation, and birth rates. A transcriptional microarray study was also performed to compare gene expression of 6-day-old rabbit embryos previously frozen and transferred into recipient rabbit females to their in vivo counterparts. Our goal was to study which alteration caused by the freezing procedure still remained in late blastocyst stage just at the time when the implantation process began. A microarray specifically designed to study rabbit gene expression profiling was used in this study. Lower implantation and birth rates were obtained in frozen embryos than in the control group (29.9% and 25.7% vs 88.5% and 70.8% for frozen and control embryos, respectively). Likewise, differences were also observed in gene expression profiles. Compared to 6-day-old in vivo-derived embryos, viable frozen embryos presented 70 differentially expressed genes, 24 upregulated and 46 downregulated. In conclusion, our findings showed that the slow freezing process affected late blastocyst development, implantation, and birth rates and that the gene expression alterations identified at late blastocyst stage could be useful in understanding the differences in developmental potential observed and the deficiencies that might hinder implantation and fetal development.

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Section: Discussionsupporting

confidence: 91%

Effects of Slow Freezing Procedure on Late Blastocyst Gene Expression and Survival Rate in Rabbit1

Saenz-de-Juano

Marco‐Jiménez

Peñaranda

et al. 2012

Biology of Reproduction

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“…After one generation without selection, to avoid the environmental maternal effect, 72 adult V16 female rabbits were obtained to be simultaneously compared with 79 female rabbits of generation 36. Detailed information concerning the cryopreservation and embryo transfer techniques used in this study are available in García-Ximénez (1993 and1996).…”

Section: Animalsmentioning

confidence: 99%

Different resource allocation strategies result from selection for litter size at weaning in rabbit does

Savietto

Cervera

Ródenas

et al. 2014

Animal

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This study examined the effect of long-term selection of a maternal rabbit line, solely for a reproductive criterion, on the ability of female rabbits to deal with constrained environmental conditions. Female rabbits from generations 16 and 36 (n = 72 and 79, respectively) of a line founded and selected to increase litter size at weaning were compared simultaneously. Female rabbits were subjected to normal (NC), nutritional (NF) or heat (HC) challenging conditions from 1st to 3rd parturition. Animals in NC and NF were housed at normal room temperatures (18°C to 25°C) and respectively fed with control (11.6 MJ digestible energy (DE)/kg dry matter (DM), 126 g digestible protein (DP)/kg DM, and 168 g of ADF/kg DM) or low-energy fibrous diets (9.1 MJ DE/kg DM, 104 g DP/kg DM and 266 g ADF/kg DM), whereas those housed in HC were subjected to high room temperatures (25°C to 35°C) and the control diet. The litter size was lower for female rabbits housed in both NF and HC environments, but the extent and timing where this reduction took place differed between generations. In challenging conditions (NF and HC), the average reduction in the reproductive performance of female rabbits from generation 16, compared with NC, was −2.26 (P < 0.05) and −0.51 kits born alive at 2nd and 3rd parturition, respectively. However, under these challenging conditions, the reproductive performance of female rabbits from generation 36 was less affected at 2nd parturition (−1.25 kits born alive), but showed a greater reduction at the 3rd parturition (−3.53 kits born alive; P < 0.05) compared with NC. The results also showed differences between generations in digestible energy intake, milk yield and accretion, and use of body reserves throughout lactation in NC, HC and NF, which together indicate that there were different resource allocation strategies in the animals from the different generations. Selection to increase litter size at weaning led to increased reproductive robustness at the onset of an environmental constraint, but failure to sustain the reproductive liability when the challenge was maintained in the long term. This response could be directly related to the shortterm environmental fluctuations (less severe) that frequently occur in the environment where this line has been selected.

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“…Only normal morulae were frozen. The freezing and thawing procedures have been described in previous studies (Garcia-Ximénez and Vicente, 1991;Vicente and Garcia-Ximénez, 1993 No differences were found in pregnancy rate between embryo strains (56%, 9/16). (Schmidt et al, 1985(Schmidt et al, , 1987Pomp and Eisen, 1990).…”

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confidence: 96%

Effect of recipient doe genotype.on survival rate at birth of frozen rabbit embryos

Vicente¹,

García-Ximénez²

1993

Reprod. Nutr. Dev.

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Summary -The effect of the genotype of 2 selected rabbit strains (White New Zealand and Synthetic) on the survival rate at birth of frozen embryos was studied. Morulae were obtained 64-66 h postcoitum from 32 multiparous does naturally mated with males of the same strain (16 does of Synthetic strain (SY) and 16 does of the White New Zealand strain (NZ)). Morphologically normal morulae were slowly frozen in 1.5 M DMSO and stored in liquid nitrogen. After thawing, intact embryos from both strains were transferred into the oviducts of 32 recipient does of both strains (16 SY strain does and 16 NZ does). Significant differences were observed in pregnancy rate and survival rate at birth between strains of recipient does. The recipient does of the SY strain showed a higher pregnancy rate than those of the NZ strain (87.5% versus 25% respectively; P < 0.05). When the survival rate at birth was analyzed in all recipient does, this was found to be 28 ± 4% in SY recipient does versus 8 ± 4 % in NZ recipient does (P< 0.01). However, when the survival rate was analyzed in pregnant recipient does, no significant differences were found between embryo strains and recipient strains, the average survival rate was 32%. The major factor in the differential survival rate between SY and NZ embryos was due to the genotype of recipient does.

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Effects of strain and embryo transfer model (embryos from one versus two donor does/recipient) on results of cryopreservation in rabbit

Abstract: Summary ― Differential effects of 2 transfer models for normal thawed embryos of 1 donor doe were studied on the offspring rate and their embryo survival at birth from 3 selected rabbit strains (SY and

Cited by 23 publications

References 13 publications

Effects of Slow Freezing Procedure on Late Blastocyst Gene Expression and Survival Rate in Rabbit1

Effects of Slow Freezing Procedure on Late Blastocyst Gene Expression and Survival Rate in Rabbit1

Different resource allocation strategies result from selection for litter size at weaning in rabbit does

Effect of recipient doe genotype.on survival rate at birth of frozen rabbit embryos

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