Effects of substrate composition on the structure of microbial communities in wastewater using fluorescence in situ hybridisation

Addison, Sarah; Slade, Alison; Dennis, Marie

doi:10.1016/j.syapm.2010.10.006

Cited by 9 publications

(3 citation statements)

References 43 publications

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“…Most previous work has focused on nitrogen fixation as a treatment of high carbon-to-nitrogen wastewater without focusing on the augmentation of biomass as a valorization method of wastewater sludge. ,,− We clearly demonstrated that the reactors, operated without any added nitrogen source, had the ability to produce biomass enriched with NFB and treat the wastewater to the same standard as reactors supplemented with nitrogen. We are confident that the nitrogen was sourced from the air and not from recycled biomass because almost no ammonium or oxidized nitrogen compounds were found in the effluent from the reactors.…”

Section: Discussionmentioning

confidence: 81%

Enrichment of Nitrogen-Fixing Bacteria in a Nitrogen-Deficient Wastewater Treatment System

Ospina‐Betancourth

Acharya

Allen

et al. 2020

Environ. Sci. Technol.

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Anthropogenic nitrogen fixation is essential to sustain a global population of 7.7 billion. However, there has been a long-standing desire to find cheaper and more environmentally friendly alternatives to the Haber−Bosch process. In this study, we developed a new strategy of nitrogen fixation by enriching free-living N 2 -fixing bacteria (NFB) in reactors fed with low nitrogen wastewater, analogous to those usually found in certain industrial effluents such as paper mills. Our reactors fixed appreciable quantities of nitrogen with a rate of 11.8 mg N L −1 day −1 . This rate is comparable to recent "breakthrough" nitrogen-fixing technologies and far higher than observed in low C/N reactors (fed with organic matter and nitrogen). NFB were quantified using quantitative polymerase chain reaction (qPCR) of the nif H (marker gene used to identify biological nitrogen fixation) and 16S rRNA genes. The nif H gene was enriched by a factor of 10 in the nitrogen-fixing reactors (compared to controls) attaining 13% of the bacterial population (1:4.2 copies of nif H to 16S rRNA). The Illumina MiSeq 16S rRNA gene amplicon sequencing of reactors showed that the microbial community was dominated (19%) by Clostridium pasteurianum. We envisage that nitrogen-enriched biomass could potentially be used as a biofertilizer and that the treated wastewater could be released to the environment with very little post-treatment.

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Section: Discussionmentioning

confidence: 81%

Enrichment of Nitrogen-Fixing Bacteria in a Nitrogen-Deficient Wastewater Treatment System

Ospina‐Betancourth

Acharya

Allen

et al. 2020

Environ. Sci. Technol.

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“…For example, the diversity and abundance of three dominant genera, Aeromonas, Pseudomonas, and Bacillus were investigated in a pulp and paper wastewater by enrichment with 15 N2 by Addison et al (2010Addison et al ( , 2011; specific probes were used to investigate and enumerate the different bacterial groups present at particular stages through the wastewater treatment system over an extended period.…”

Section: Fluorescent In Situ Hybridization (Fish)mentioning

confidence: 99%

Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

et al. 2017

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One of the processes most used in biotechnology today for handling industrial liquid wastes is biological wastewater treatment. The efficiency and quality of its operation depends on the composition and activity of the microbial community that is present. The application of traditional and molecular techniques has provided a glimpse into the "black box" and has given information to improve the wastewater treatment process. However, bleach pulp and paper mill effluents require a better understanding of the active bacterial population. For the study of these microorganisms, molecular techniques have been used for more than 15 years. However, there has been a lack of knowledge of the physiological requirements and relations with the environment, which seems to be very difficult to obtain involving profile on the diversity. Nowadays, highthroughput sequencing technology is a promising method that makes it possible to identify the entire profile of microbial communities. In combination with fingerprint methods, this approach allows the identification and analysis of the whole biodiversity of microbial communities. In this review, several identification techniques will be discussed.

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“…The Bacteroides phylum also had a major advantage over the other microorganisms in terms of wastewater treatment. It can degrade complex organic matter and promote the hydrolysis and acidification of macromolecular organic matter into small molecular substances, e.g., acetic acid and propionic acid (Addison et al 2011). Actinomycota played an important role in the degradation of macromolecular complex organics, e.g., aromatic compounds, cellulose, lignins, etc.…”

Section: Study On the Co-metabolism Floramentioning

confidence: 99%

Treatment of nitrate-nitrogen-containing wastewater via aerobic denitrifying bacteria using different carbon sources

Lan

Lai

Wang

et al. 2022

BioRes

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The effect of different carbon sources was studied relative to the treatment effect of aerobic denitrifying bacteria during the treatment of nitrate-containing wastewater as well as the influence of co-metabolism flora. Three carbon sources, i.e., glucose, citric acid, and sodium acetate, were selected to study the changes in the pH, nitrate nitrogen, chemical oxygen demand removal, nitrite nitrogen concentration, physical and chemical properties of sludge and volatile fatty acids, and high-throughput sequencing to study any changes in flora. The results showed that the denitrification ability of the system using citric acid as the carbon source was stronger than the denitrification ability of the system using glucose or sodium acetate as the carbon source. The removal of nitrate nitrogen in the system was the result of the co-metabolism of acid producing bacteria and aerobic denitrifying bacteria. Due to the greater number of types and greater amount of dominant bacteria in the sludge domesticated and cultured with citric acid as a carbon source, the co-metabolism flora formed was more stable, so it could better remove nitrate nitrogen.

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Effects of substrate composition on the structure of microbial communities in wastewater using fluorescence in situ hybridisation

Cited by 9 publications

References 43 publications

Enrichment of Nitrogen-Fixing Bacteria in a Nitrogen-Deficient Wastewater Treatment System

Enrichment of Nitrogen-Fixing Bacteria in a Nitrogen-Deficient Wastewater Treatment System

Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

Treatment of nitrate-nitrogen-containing wastewater via aerobic denitrifying bacteria using different carbon sources

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