Effects of Synechococcus sp. PCC 7942 harboring vp19, vp28, and vp (19 + 28) on the survival and immune response of Litopenaeus vannamei infected WSSV

Zhu, Chan; Shi, Dingji; Liao, Shengyu; He, Peimin; Jia, Rui

doi:10.1016/j.fsi.2020.01.028

Cited by 13 publications

(10 citation statements)

References 38 publications

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“…Superoxide dismutase (SOD), catalase (CAT), lysozyme (LYZ), and phenoloxidase (PO) are four important immune enzymes, which have the function of anti-oxidant and anti-pathogen (Holmblad and Söderhäll, 1999;Cerenius and Söderhäll, 2004;Kaizu et al, 2011). The activity of these immune enzymes is usually used to reflect the impact of shrimp immunity after a pathogen infection (such as WSSV, IHHNV, Vibrio parahaemolyticus, and Spiroplasma eriocheiris) (Arockiaraj et al, 2012;Ren et al, 2020;Zhu et al, 2020;Jiao et al, 2021), harmful substance invasion (such as microcystin and tributyltin) (Wu et al, 2014;Chen et al, 2015), or dietary improvement (such as polypeptides and dietary hydrolyzable tannins) (Liao et al, 2019;Zhu et al, 2021). With the rapid development of high-throughput RNA sequencing technologies, including RNA sequencing (RNA-seq), more and more studies use RNA-seq to expose the immune molecular mechanism of shrimp against viral infections, bacterial infections, and environmental stress (Kurita and Nakajima, 2012;Wang et al, 2014;Zeng et al, 2014;Qin et al, 2018;Jin and Zhu, 2019;Nian et al, 2019;Duan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Zhao

Chen

et al. 2021

Front. Microbiol.

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As a new type of shrimp lethal virus, decapod iridescent virus 1 (DIV1) has caused huge economic losses to shrimp farmers in China. Up to now, DIV1 has been detected in a variety of shrimps, but there is no report in Marsupenaeus japonicus. In the current study, we calculated the LC50 to evaluate the toxicity of DIV1 to M. japonicus and determined through nested PCR that M. japonicus can be the host of DIV1. Through enzyme activity study, it was found that DIV1 can inhibit the activities of superoxide dismutase, catalase, lysozyme, and phenoloxidase, which could be a way for DIV1 to achieve immune evasion. In a comprehensive study on the transcriptomic changes of M. japonicus in response to DIV1 infection, a total of 52,287 unigenes were de novo assembled, and 20,342 SSR markers associated with these unigenes were obtained. Through a comparative transcriptomic analysis, 6,900 differentially expressed genes were identified, including 3,882 upregulated genes and 3,018 downregulated genes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that some GO terms related to virus invasion, replication, and host antiviral infection were promoted under DIV1 infection, such as carbohydrate binding, chitin binding, chitin metabolic process, and DNA replication initiation, and some KEGG pathways related to immune response were significantly influenced by DIV1 infection, including Toll and IMD signaling pathway, JAK-STAT signaling pathway, IL-17 signaling pathway, C-type lectin receptor signaling pathway, complement and coagulation cascades, antigen processing and presentation, necroptosis, apoptosis, NOD-like receptor signaling pathway, apoptosis—multiple species, and TNF signaling pathway. Further analysis showed that STAT, Dorsal, Relish, heat shock protein 70 (HSP70), C-type lectins, and caspase play an important role in DIV1 infection. This is the first detailed study of DIV1 infection in M. japonicus, which initially reveals the molecular mechanism of DIV1 infection in M. japonicus by using the transcriptome analysis of hemocytes combined with enzyme activity study.

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Section: Introductionmentioning

confidence: 99%

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Zhao

Chen

et al. 2021

Front. Microbiol.

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“…WSSV was obtained from the Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, China, for the viral challenge in L. vannamei. Macrobrachium nipponensis (Shanghai Guoyuan, China) was used to rejuvenate the virus, moribund individuals were collected, and prawn muscle was cut into small pellets and stored at -80°C (23).…”

Section: Experimental Materialsmentioning

confidence: 99%

Construction of an easily detectable transgenic Synechococcus elongatus PCC 7942 against White Spot Syndrome Virus using vp28 and mOrange Gene and its metabolism in shrimp

et al. 2022

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White spot syndrome is an epidemic disease caused by the highly contagious and lethal white spot syndrome virus (WSSV), resulting in huge economic losses to the global aquaculture industry. VP28 is the main structural protein in the capsule of WSSV and is important in the early stage of infection. Under an excitation wavelength of 548 nm, the mOrange fluorescent protein releases a 562 nm emission wavelength, which is different from the autofluorescence of cyanobacteria. Therefore, using this characteristic combined with the receptor system of Synechococcus elongatus PCC 7942, we constructed transgenic S. elongatus to express the recombinant protein VP28-mOrange. In addition, PCR and western blotting were used to confirm the stable expression of the target gene in cyanobacteria. Using mOrange tracer features, we explored the recombinant protein VP28-mOrange in the metabolic cycle of young Litopenaeus Vannamei after feeding. After the young shrimp had stopped consuming transgenic cyanobacteria, the 24 to 33 h fluorescence signal in the intestine was very weak, and almost disappeared after 36 h. We explored the protective effect of transgenic vp28-mOrange S. elongatus within 48 h of being ingested by L. vannamei and set WSSV challenges at 2, 12, 24, and 48 h post-immunization. However, the survival rate of L. vannamei decreased as the time of the WSSV challenge increased. The survival rate on the seventh day was 81%, 52%, 45.5%, and 33.3% for shrimps challenged for 2, 12, 24, and 48 h, respectively. Enzyme activity can also support this conjecture, the enzyme activity indexes of the experimental groups were significantly reduced compared to positive and wild-type controls. Therefore, this immune agent functioned as a preventive agent. Compared with the traditional method, this method was easy to detect and can visualize the digestion of transgenic cyanobacteria in the Litopenaeus vannamei intestine.

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“…The exterior is built with green bricks. The wooden building body is a three-story wooden structure with a height of 27.4 m (Qiu et al, 2020;Xie et al, 2020;Zhu et al, 2020;Hou et al, 2021).…”

Section: General Descriptionmentioning

confidence: 99%

Detection of cracks in cemented loess of ancient buildings using remote sensing

Liu

Bao

et al. 2022

Front. Mater.

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The bases of ancient Chinese buildings are prone to deformation, cracks, and other hidden problems due to their age and other reasons. Rainfall and water seepage produce great harm to the cracks of soil plinths. Based on a ground-penetrating radar method, detecting cracks and water erosion defects is important for protecting ancient buildings. This study examines the reflection characteristics of ground-penetrating radar (GPR) waves at different lithological interfaces. Physical experiments and their numerical and physical laws were used to study the reflection characteristics of GPR incidents from brick to air, unsaturated soil, water, and metal interfaces. The model was applied to detect defects of the Xi’an Bell Tower and the main research results are as follows. The echo amplitude of GPR was positively correlated with the relative permittivity of the bedding layer, and its basic law conforms to the positive logarithmic curve. The hyperbolic opening of water erosion imaging of the base-compacted soil is larger, the attenuation effect increases and the signal reflection is obvious. These results provide the theoretical basis and technical support for the actual detection of water erosion deterioration of loess in similar projects, and this provides theoretical and technical references for rapid identification and management of defects in ancient buildings.

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Effects of Synechococcus sp. PCC 7942 harboring vp19, vp28, and vp (19 + 28) on the survival and immune response of Litopenaeus vannamei infected WSSV

Cited by 13 publications

References 38 publications

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Construction of an easily detectable transgenic Synechococcus elongatus PCC 7942 against White Spot Syndrome Virus using vp28 and mOrange Gene and its metabolism in shrimp

Detection of cracks in cemented loess of ancient buildings using remote sensing

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