Effects of temperature and salinity during incubation on hatching and yolk utilization of Gulf killifish Fundulus grandis embryos

Brown, Charles A.; Gothreaux, C. T.; Green, Christopher C.

doi:10.1016/j.aquaculture.2011.02.041

Cited by 38 publications

(24 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although no deformed individuals were observed, sub‐optimal temperatures can slow development, lead to increased rates of developmental deformities and cause mortalities (Lindén et al , ; Das et al , ; Jordaan et al , ). Fundulus grandis embryos incubated in water at a similar range of temperatures displayed decreasing per cent hatch as incubation temperature decreased (Brown et al , ), with a negative relationship of time to first hatch over a temperature gradient similar to this study (20, 21·6, 23 and 29° C).…”

Section: Discussionsupporting

confidence: 78%

Metabolic and embryonic responses to terrestrial incubation of Fundulus grandis embryos across a temperature gradient

Brown

Green

2014

Journal of Fish Biology

View full text Add to dashboard Cite

This study simulated terrestrial incubation and measured rates of embryogenesis, nitrogen elimination, heart rate, lactate production, maximum length of time a hatch could be delayed and developmental responses of terrestrially incubated Gulf killifish Fundulus grandis embryos at temperatures ranging from 20 to 30° C. Temperature had a positive relationship with rate of embryogenesis, but a negative relationship with extent of extended incubation. The 30° C treatment reached embryonic maturity 6 days before the 20° C treatment. Embryos hatched between intervals of 240 and 336, 144 and 288, 96 and 240 and 96 and 192 h after reaching developmental maturity for the 20, 23, 26 and 30° C treatments. Significantly higher concentrations of total nitrogen, in the form of ammonia and urea, were recorded in the 20 and 30° C treatments. While temperature significantly influenced lactate and ATP concentrations, no significant influence of time of incubation was detected. Terrestrial embryos displayed an ability to develop quickly during embryogenesis and prolong incubation for an extended period of time after reaching embryonic maturity. This adaptation may be a life-history trait used to minimize asynchronous hatching, cannibalism and cohort size heterogeneity.

show abstract

Section: Discussionsupporting

confidence: 78%

Metabolic and embryonic responses to terrestrial incubation of Fundulus grandis embryos across a temperature gradient

Brown

Green

2014

Journal of Fish Biology

View full text Add to dashboard Cite

show abstract

“…Comparativamente, la mayoría de los embriones de peces de agua dulce son estenohalinos (Gbulubo & Erondu, 1998;Ekmekçi, 2002;FashinaBombata & Busari, 2003;Albert et al, 2004;Bhattacharya et al, 2006), no superando generalmente el umbral de salinidad de 10 por breves períodos de exposición. En cambio los peces eurihalinos soportan amplios gradientes salinos (Mihelakakis & Kitajima, 1994;Hart & Purser, 1995;Smith et al, 1999;Berlinsky et al, 2004;Yang & Chen, 2006;Zhang et al, 2010;Brown et al, 2011) por períodos prolongados de exposición. Una diferencia observada en el embrión de G. maculatus respecto de otros embriones de peces eurihalinos fue su capacidad para completar la ontogenia embrionaria en agua dulce, sin que la ausencia de sal influyera en los porcentajes de supervivencia embrionaria (entre los tratamientos a salinidades de 0, 4, 8, 12, 16 y 20 no hubo diferencias significativas).…”

Section: Discussionunclassified

Efecto de la salinidad en la supervivencia embrionaria de puye Galaxias maculatus (Jenyns, 1842)

Barile¹,

Escudero²,

Carreño³

et al. 2015

lajar

View full text Add to dashboard Cite

RESUMEN.Se evaluó e l efecto de la salinidad en la supervivencia embrionaria, período embrionario y período de eclosión de puye, Galaxias maculatus. Se utilizaron ocho tratamientos, con salinidades de 0, 4, 8, 12, 16 y 20 obteniéndose supervivencias embrionarias promedios respectivas de 84,9; 84,0; 85,5; 86,1; 82,6 y 75,8%, sin diferencias significativas entre ellas, mientras que con salinidad de 24 se obtuvo sobrevivencia de 10,4%, significativamente diferente al resto de los tratamientos y con salinidad de 28 causó 100% de mortalidad. A diferencia del estado juvenil y adulto, el embrión es incapaz de resistir la salinidad marina y su umbral de tolerancia se encuentra entre salinidades de 24 y 28. Las mayores supervivencias promedios larvales a 10 días de vida fueron con salinidades intermedias de 8, 12 y 16 de 79,3; 80,3 y 74,6% respectivamente, sin diferencias significativas entre ellas, demostrando alta viabilidad del embrión post-eclosión. Los períodos embrionarios promedios (50%) más breves fueron a bajas salinidades 0, 4, 8, 12 con 28,7; 28,0; 29,7 y 29,7 días y los más extensos en los tratamientos con salinidades de 16 y 20, con 34,0 y 34,3 días, sin diferencias significativas entre ellos. Los períodos de eclosión promedio (50%) con salinidades de 0, 4, 8, 12, 16, 20 y 24 fueron de 5,0; 5,5; 7,5; 8,8; 13,0; 14,3 y 15,8 días respectivamente, sin diferencias significativas entre ellos. Palabras clave: Galaxias maculatus, puye, eclosión, incubación, tolerancia salina, acuicultura. Effect of salinity on survival of embryos of jollytailGalaxias maculatus (Jenyns, 1842)ABSTRACT. Eight different salinity effects on embryonic survival and embryonic and hatching phase time duration of jollytail (Galaxias maculatus) were assessed. Mean survival rates were higher at salinities values of 0, 4, 8, 12, 16 and 20 with 84.9, 84.0, 85.5, 86.1, 82.6 and 75.8% respectively with no significant differences between them; in contrast to salinity of 24 was obtained by 10.4%, significantly different from other treatments. Salinity of 28 caused 100% of mortality. According to results, the embryo is unable to resist ocean salinity and its tolerance limit is between 24 and 28. The highest larval survivals at 10 days after hatching occurred in those treatments in which embryos were performed to intermediate salinities of 8, 12 and 16 with 79.3, 80.3 and 74.6% respectively, with no significant differences between them. These results demonstrate high post-hatching viability. Embryonic time period (50%) were shorter at low salinities 0, 4, 8 and 12 with 28.7, 28.0, 29.7 and 29.7 days and the largest, in the treatments of salinities of 16 and 20 with 34.0 and 34.3 days. No significant differences were found between all of them. Regarding the average hatching time periods (50%) in the treatments of salinities of 0, 4, 8, 12, 16, 20 and 24 were 5.0, 5.5, 7.5, 8.8, 13, 0, 14.3 and 15.8 days respectively, without significant differences between them.

show abstract

“…Eggs were fertilized as described by Brown et al (2011). Newly fertilized eggs were soaked in a 2 g/L solution of sodium sulfite for *15 min to decrease adhesion (Stone and Ludwig 1993).…”

Section: Methodsmentioning

confidence: 99%

“…Larvae were preserved in 10% buffered formalin, and time-to-hatch (TH) from fertilization was recorded. Total length (TL), bodydepth-at-vent (BD), and body cavity area (BCA) were determined for these larvae with techniques described by Brown et al (2011). BCA was measured instead of yolk volume due to the inability to distinguish yolk from the abdominal cavity.…”

Section: Heart Rate Samplingmentioning

confidence: 99%

Embryonic development and metabolic costs in Gulf killifish Fundulus grandis exposed to varying environmental salinities

Brown

Gálvez

Green

2012

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

The Gulf killifish (Fundulus grandis) is a euryhaline fish found in coastal marsh along the entire of Gulf of Mexico and southern Atlantic of coast of the United States. The objective of this study was to investigate the effects of salinity on embryogenesis in the Gulf killifish. Four recirculation systems at salinities of 0.4, 7, 15, and 30 g/L were maintained at a static temperature with flow-through trays, containing embryos (n = 39) placed in triplicate into each system. Throughout embryogenesis, the rate of development, ammonia and urea excretion, and heart rate were monitored. Percent hatch was recorded, and morphological parameters were measured for larvae at hatch. As salinity was increased, the rate of embryogenesis decreased. Salinity significantly affected percent hatch with an 80.0% ± 2.6% for 7 g/L and 39.1 ± 4.3, 45.4 ± 4.5, and 36.3% ± 12.0% for 0.4, 15, and 30 g/L, respectively. Salinity and stage of development significantly affected production of ammonia and urea. As salinity increased, the dominate metabolite end product changed from urea to ammonia. However, the 15 g/L salinity treatment had the two highest levels of urea recorded. Heart rate was unaffected by salinity but increased throughout embryogenesis and remained constant once embryos reached stages where hatching has been recorded. While mean total length was not affected by salinity, embryos incubated in 30 g/L produced larvae with significantly thicker body depth at hatch. The 0.4, 7, and 15 g/L salinity treatments all had similar mean hours to hatch. The 30 g/L treatment resulted in a significantly longer mean time to hatch and smaller body cavity area at hatch.

show abstract

Effects of temperature and salinity during incubation on hatching and yolk utilization of Gulf killifish Fundulus grandis embryos

Cited by 38 publications

References 42 publications

Metabolic and embryonic responses to terrestrial incubation of Fundulus grandis embryos across a temperature gradient

Metabolic and embryonic responses to terrestrial incubation of Fundulus grandis embryos across a temperature gradient

Efecto de la salinidad en la supervivencia embrionaria de puye Galaxias maculatus (Jenyns, 1842)

Embryonic development and metabolic costs in Gulf killifish Fundulus grandis exposed to varying environmental salinities

Contact Info

Product

Resources

About