Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development

Feng, Dingqing; Zhou, Yong; Ling, Bin; Gao, Tingting; Yu, Shichong; Wei, Hang; Tian, Zhi Gang

doi:10.1590/s0100-879x2009000600006

Cited by 7 publications

(16 citation statements)

References 41 publications

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“…In this regard, Feng et al 22 showed that MSC-CM can efficiently stimulate mouse oocyte by the generation of Ca 2+ oscillations and finalization of meiosis. In this regard, Feng et al 22 showed that MSC-CM can efficiently stimulate mouse oocyte by the generation of Ca 2+ oscillations and finalization of meiosis.…”

Section: Discussionmentioning

confidence: 99%

“…11,17 Information regarding the appropriate conditions for human IVM is still minimal. 22,23 EGF and IGF-1 are thought to play important roles in the improvement of meiotic maturation, directly or via CCs. 14 Culture conditions are thought to be the most essential factor affecting IVM efficacy.…”

Section: Introductionmentioning

confidence: 99%

“…18 Because changing the composition of culture media can affect the oocyte maturation and their subsequent embryonic development, 19 many different types of commercially maturation media have been used clinically. 22,24 Because the results showed less developmental potential of in vitro matured oocytes compared with those of matured in vivo, 15 and this problem in PCOS condition is more intense, in the current study, we aimed to use human bone marrow MSC-conditioned media (hBM-MSC-CM), as a supplement, to improve the IVM culture medium for oocyte IVM from mice with polycystic ovary syndrome. 20,21 MSCs secrete a variety of cytokines and growth factors, such as insulin-like growth factor-1 (IGF-1), VEGF, EGF, fibroblast growth factor (FGF), interleukin-6, leukemia inhibitory factor (LIF), TGF-β, and so on.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell–conditioned media

Jafarzadeh

Nazarian

Novin

et al. 2018

J of Cellular Biochemistry

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The outcome of in vitro maturation (IVM) in the patients with polycystic ovary syndrome (PCOS) is poor. Abnormal intraovarian paracrine interplay alters microenvironment for oocyte development through folliculogenesis and decreases developmental competence of oocytes in patients with PCOS. Mesenchymal stromal cells (MSCs) secrete a variety of cytokines and growth factors that could promote oocyte maturation in vitro. Thus, in the current study we aimed to evaluate the effect of human bone marrow MSC-conditioned media (hBM-MSC-CM), as a supplement, to enrich IVM medium for PCOS germinal vesicles (GVs). For this purpose, oocytes at GV and metaphase II (MII) stages were harvested from PCOS mice. The GVs were randomly divided into four groups and incubated for 24 hours in an IVM medium (TCM199, as the control group) or TCM199 supplemented by 25%, 50%, and 75% of hBM-MSC-CM (PCOS-CM25, PCOS-CM50, and PCOS-CM75 groups, respectively) so as to evaluate which dose(s) could enhance maturation rate of the GVs and their subsequent in vitro fertilization (IVF) outcome. Furthermore, MII oocytes and their subsequent IVF outcome were considered as the in vivo matured (PCOS-IVO) group. The data showed that supplementation of IVM medium with 50% hBM-MSC-CM significantly increased cytoplasmic and nuclear maturation of the GVs (P < 0.001), and also fertilization and two-cell rate (P < 0.001) and blastocyst formation (P < 0.01) of in vitro matured oocytes from mice with PCOS. Overall, higher oocyte maturation and fertilization outcome in PCOS-CM50 group proposed that enrichment of IVM medium with hBM-MSC-CM could be considered as a promising approach to improve IVM of PCOS oocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell–conditioned media

Jafarzadeh

Nazarian

Novin

et al. 2018

J of Cellular Biochemistry

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“…Secretome components secreted by MSCs, such as chemokines, cytokines, and growth factors, are crucial to maintain the homeostasis and signal transduction with stromal cells (fibroblast, endothel cell, macrophage) [37]. These soluble factors in CM determine the fate of stem cells in in-vitro assays [38].…”

Section: Effect Of CM From Co-cultured Hwjmscs and Il-2 Nk Cells Towamentioning

confidence: 99%

Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7)

et al. 2019

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Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton's jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-α, IFN-γ, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression.

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“…MSCs secrete secretome such as chemokins, cytokines, growth factor as bioactive and nutrition 50 , these secretome are important to preserve the homeostasis, cross talk with stromal cells (fibroblast, endothel cell, macrophage). The soluble factor of CM affect stem cells fate by in vitro assay.…”

Section: Effect CM From Co-culture Of Hwjmscs and Ils-nk Towards Apopmentioning

confidence: 99%

Direct and Indirect Effect of TNFα and IFNγ Toward Apoptosis in Breast Cancer Cells

Widowati

Jasaputra

Sumitro

et al. 2018

Mol Cell Biomed Sci

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Background: Breast cancer (BC) is the leading cause of death cancer in women. Cancer therapies using TNFα and IFNγ have been recently developed by direct effects and activation of immune responses. This study was performed to evaluate the effects of TNFα and IFNγ directly, and TNFα and IFNγ secreted by Conditioned Medium-human Wharton’s Jelly Mesenchymal Stem Cells (CM-hWJMSCs) toward apoptosis of BC cells (MCF7).Materials and Methods: BC cells were induced by TNFα and IFNγ in 175 and 350ng/mL, respectively. CM-hWJMSCs were produced by co-culture hWJMSCs and NK cells that secreted TNFα, IFNγ, perforin (Prf1), granzyme B (GzmB) for treating BC cells. The BC cells were treated with CM-hWJMSCs in 50%. The expression of apoptotic genes Bax, p53, and the antiapoptotic gene Bcl-2 were determined using RT-PCR.Results: TNFα and IFNγ at concentration of 350 ng/mL induced higher Bax expression compared to 175 ng/mL. TNFα and IFNγ 350 ng/mL, 175 ng/mL induced p53 expression, whilst TNFα and IFNγ at 350 ng/mL decreased Bcl-2 expression. Perf1, GzmB, TNFα and IFNγ-containing CM-hWJMSCs induced significantly apoptosis percentage, induced Bax expression, but did not effect p53, Bcl-2 expression.Conclusion: TNFα and IFNγ directly induce Bax, p53, decrease Bcl-2 gene expression. The Prf1, GzmB, TNFα, IFNγ-containing CM-hWJMSCs induce apoptosis and Bax expression.Keywords: breast cancer, Wharton’s Jelly mesenchymal stem cells, TNFα, IFNγ

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Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development

Cited by 7 publications

References 41 publications

Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell–conditioned media

Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell–conditioned media

Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7)

Direct and Indirect Effect of TNFα and IFNγ Toward Apoptosis in Breast Cancer Cells

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