Effects of trans-2-nonenal and olfactory masking odorants on proliferation of human keratinocytes

Nakanishi, Satoshi; Makita, Mio; Denda, Mitsuhiro

doi:10.1016/j.bbrc.2021.02.050

Cited by 5 publications

(10 citation statements)

References 37 publications

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“…Olfactory masking is a distinctive feature of olfaction. Recently, we observed this phenomenon in human keratinocytes 108 (Figure 1).…”

Section: Olfactory Stimulusmentioning

confidence: 62%

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Do epidermal keratinocytes have sensory and information processing systems?

Denda

Nakanishi²

2021

Experimental Dermatology

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Epidermal keratinocytes are well known to form a water-impermeable membrane, the stratum corneum, as a physical protective barrier at the surface of the skin. In contrast, studies on cutaneous sensation, for example, tactile sensation or temperature sensation, have focused on the role of nerve terminals or nerve fibres in the skin. However, in the past two decades, sensory functions of keratinocytes for many kinds of environment factors have been discovered. In addition, it has been found that keratinocytes express neurotransmitters and their receptors. These discoveries have dramatically changed our understanding of the role of the epidermis in human skin. Here, we review the sensory and information processing capabilities of keratinocytes, and we discuss the possible role of the epidermis from the standpoint of vertebrate evolution. We suggest that epidermal keratinocytes influence the physical and emotional health of the whole body. | BACKG ROU N DWe have shown that a variety of physical and chemical factors influence epidermal water-impermeable barrier homeostasis. 1 Furthermore, it was reported that the epidermal Ca gradient regulates intracellular Ca distribution and plays a key role in maintaining the barrier function. 2,3 Based on these reports, we searched for corresponding receptors in keratinocytes, and identified a variety of receptors that were originally found in sensory organs and the nervous system, including the brain. 1 Moreover, many other sensory

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“…Olfactory masking is a distinctive feature of olfaction. Recently, we observed this phenomenon in human keratinocytes 108 (Figure 1).…”

Section: Olfactory Stimulusmentioning

confidence: 62%

“…Olfactory masking is a distinctive feature of olfaction. Recently, we observed this phenomenon in human keratinocytes 108 (Figure 1). It has been proposed that olfactory masking occurs not only in the olfactory bulb or brain, but also at the cell level 109‐111 .…”

Section: Sensory System For Chemical Phenomenamentioning

confidence: 66%

Do epidermal keratinocytes have sensory and information processing systems?

Denda

Nakanishi²

2021

Experimental Dermatology

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“…15 Nakanishi et al also reported that the induction of apoptosis and reduction of viability of human keratinocytes by NE is blocked by specific masking odors, even though keratinocytes are not olfactory cells. 10 The phospholipid artificial membrane is used as a model cell membrane to investigate the mechanism of cell response because it is difficult to factor out intrinsic elements and their nature on the mechanism of chemical sensation in living organisms as a result of their complexity. 16−20 Indeed, interactions between chemical species and functional groups in phospholipid artificial membranes have been reported to be associated with barrier homeostasis of cellular membranes in skin.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The previous report demonstrated that, among body odor components, only NE in skin surface stratum corneum obviously increases with aging, and another report indicated that the recovery of the stratum corneum after its disruption is delayed with aging . Nakanishi et al reported that NE is cytotoxic to human keratinocytes and induces abnormal cornification …”

Section: Introductionmentioning

confidence: 99%

Effects of the Molecular Structure of Malodor Substances and Their Masking on 1,2-Dioleoyl-sn-glycero-3-phosphocholine Molecular Layers

Yotsumoto,

Fujita,

Matsuo

et al. 2024

Langmuir

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Certain odors have been shown not only to cause health problems and stress but also to affect skin barrier function. Therefore, it is important to understand olfactory masking to develop effective fragrances to mask malodors. However, olfaction and olfactory masking mechanisms are not yet fully understood. To understand the mechanism of the masking effect that has been studied, the responses of several target substance (TS) molecules−1,2-dioleoyl-sn-glycero-3phosphocholine (DOPC) mixed molecular layers to odorant (OD) molecules were examined as a simple experimental model of epithelial cellular membranes injured by TS molecules. Here, we examined trans-2-nonenal, 1-nonanal, trans-2-decenal, and 1-decanal as TS molecules to clarify the effects of double bonds and hydrocarbon chain lengths on the phospholipid molecular layer. In addition, benzaldehyde and cyclohexanecarboxaldehyde were utilized as OD molecules to clarify the masking effect of the aromatic ring. Surface pressure (Π)−area (A) isotherms were measured to clarify the adsorption or desorption of TS and OD molecules on the DOPC molecular layer. In addition, Fourier transform infrared spectroscopy was performed to clarify the interactions among DOPC, TS, and OD molecules. We found that TS molecules with and without double bonds had different effects on the DOPC molecular layer and that molecules with shorter chain lengths had greater effects on the DOPC molecular layer. Furthermore, OD molecules with aromatic rings counteracted the effects of the TS molecules. On the basis of this research, not only a detailed mechanism by which odor molecules affect lipid membranes without mediating olfactory receptors is elucidated but also more effective OD molecules with masking effects are proposed.

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“…It was previously shown that trans -2-nonenal covalently modified through reaction of aldehyde with lysine side-chain amino groups and 2-nonenal-lysine adducts was confirmed during lipid peroxidation-mediated modification of protein in vitro and in vivo [ 6 ]. Furthermore, trans -2-nonenal was injurious to keratinocytes through promoted apoptosis and reduced the thickness and the number of proliferative cells in a three-dimensional epidermal equivalent model [ 7 ]. Therefore, we hypothesize that the protective effects against trans -2-nonenal on keratinocytes might involve a process of chemical inactivation through its structural modification.…”

Section: Introductionmentioning

confidence: 99%

Kukoamine B from Lycii Radicis Cortex Protects Human Keratinocyte HaCaT Cells through Covalent Modification by Trans-2-Nonenal

Kim

et al. 2022

Plants

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The unsaturated aldehyde trans-2-nonenal is known to be generated by lipid peroxidation at the surface of the skin in an aging-related manner and has harmful effects on keratinocytes in the skin. In this study, the protective effect of a Lycii Radicis Cortex (LRC) extract against trans-2-nonenal-induced cell damage on human keratinocyte cell lines (HaCaT) was investigated. Notably, treatment with the LRC extract resulted in an increase in cell survival, while trans-2-nonenal decreased the viability of HaCaT cells. For identification of interaction between the LRC extract and trans-2-nonenal, this mixture was incubated in simulated physiological conditions, showing a strong decrease in the amount of trans-2-nonenal by the LRC extract. Subsequent LC-ESI-MS analysis revealed that kukoamine B (KB) formed Schiff base-derived pyridinium adducts with trans-2-nonenal. Thus, these results suggest that KB could be a potential agent that may protect HaCaT cells by forming new products with trans-2-nonenal.

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Effects of trans-2-nonenal and olfactory masking odorants on proliferation of human keratinocytes

Cited by 5 publications

References 37 publications

Do epidermal keratinocytes have sensory and information processing systems?

Do epidermal keratinocytes have sensory and information processing systems?

Effects of the Molecular Structure of Malodor Substances and Their Masking on 1,2-Dioleoyl-sn-glycero-3-phosphocholine Molecular Layers

Kukoamine B from Lycii Radicis Cortex Protects Human Keratinocyte HaCaT Cells through Covalent Modification by Trans-2-Nonenal

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