Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells

Yue, Qing-Xi; Song, Xiao-Yi; Ma, Chao; Ling, Feng; Guan, Shu‐Hong; Wu, Wanying; Yang, Min; Jiang, Baohong; Liu, X.; Cui, Yaokui; Guo, Di

doi:10.1016/j.phymed.2009.12.013

Cited by 91 publications

(49 citation statements)

References 35 publications

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“…GAs are a subtype of triterpenes with four cyclic and two linear isoprenes (Liu et al, 2012). There are over 140 species of GAs that have been identified from G. lucidum (Yue et al, 2010). The core molecular structure of GA is shown in Fig.…”

Section: Triterpenesmentioning

confidence: 99%

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

et al. 2015

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Section: Triterpenesmentioning

confidence: 99%

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

et al. 2015

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“…The mixture of triterpenoids naturally occurring in G. lucidum inhibits the proliferation of human and mouse carcinoma cell lines (6). Studies have reported that the cytotoxicity mediated by triterpene-enriched extracts of G. tsugae in MCF-7 human breast cancer, prostate cancer and PC-3 cells occurs via apoptosis and cell-cycle arrest (7)(8)(9). Another study demonstrated that apoptosis induced by triterpene-enriched extracts of G. lucidum occurs through the suppression of protein kinase C, the activation of mitogen-activated protein kinases (MAPKs) and G2-phase cell-cycle arrest (10).…”

Section: Introductionmentioning

confidence: 99%

Induction of apoptosis in MCF-7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium)

Kim

et al. 2015

Molecular Medicine Reports

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Abstract. Khz (fusion of Ganoderma lucidum and Polyporus umbellatus), isolated from the mycelia of G. lucidum and P. umbellatus, exerts anti-proliferative effects against malignant cells; however, its activity against human breast cancer cells remains to be elucidated. In the present study, cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and poptosis was examined using annexin V-propidium iodide staining and flow cytometry. The activation of caspases 7, 8 and 9 were detected in the Khz-treated cells using western blotting. The results demonstrated that Khz increased the intracellular calcium concentration and induced the production of reactive oxygen species in MCF-7 breast cancer cells, as determined using flow cytometry. The results also demonstrated that Khz inhibited cell proliferation and induced apoptosis in the MCF-7 cells. In addition, the mechanism by which Khz induces apoptosis in cancer cells was investigated. Khz induced apoptosis preferentially in transformed cells, with a minimal effect on non-transformed cells, suggesting its potential as an anticancer therapeutic agent. Oxidative stress is associated with apoptotic and non-apoptotic cell death, although pro-oxidative conditions are not a pre-requisite for apoptosis. Assessment of the activation status of caspases 7, 8 and 9 revealed that the levels of cleaved caspases were significantly increased in the cells treated with Khz. It is widely accepted that calcium signaling is important in apoptosis, and the present study observed an increase in [Ca 2+ ] i in response to Khz treatment. The anti-proliferative and pro-apoptotic effects of Khz suggest that this extract may be developed as a potential anticancer agent.

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“…Modern pharmacological and clinical research has demonstrated that G. lucidum has significant antitumor, antiviral, antihypertensive, and immunomodulatory activities (2,3). These pharmaceutical activities come from the bioactive compounds of G. lucidum.…”

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confidence: 99%

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

Zhang

Ren

et al. 2016

Appl Environ Microbiol

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Heat stress (HS) influences the growth and development of organisms. Thus, a comprehensive understanding of how organisms sense HS and respond to it is required. Ganoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system due to the complete sequencing of its genome, transgenic systems, and reliable reverse genetic tools. In this study, we found that HS inhibited mycelium growth, reduced hyphal branching, and induced the accumulation of ganoderic acid biosynthesis and heat shock proteins (HSPs) in G. lucidum. Our data showed that HS induced a significant increase in cytosolic Ca 2؉ concentration. Further evidence showed that Ca 2؉ might be a factor in the HS-mediated regulation of hyphal branching, ganoderic acid (GA) biosynthesis, and the accumulation of HSPs. Our results further showed that the calcium-permeable channel gene (cch)-silenced and phosphoinositide-specific phospholipase gene (plc)-silenced strains reduced the HS-induced increase in HSP expression compared with that observed for the wild type (WT). This study demonstrates that cytosolic Ca 2؉ participates in heat shock signal transduction and regulates downstream events in filamentous fungi. IMPORTANCEGanoderma lucidum, a higher basidiomycete with bioactive secondary metabolites, has become a potential model system for evaluating how environmental factors regulate the development and secondary metabolism of basidiomycetes. Heat stress (HS) is an important environmental challenge. In this study, we found that HS inhibited mycelium growth, reduced hyphal branching, and induced HSP expression and ganoderic acid biosynthesis in G. lucidum. Further evidence showed that Ca 2؉ might be a factor in the HS-mediated regulation of hyphal branching, GA biosynthesis, and the accumulation of HSPs. This study demonstrates that cytosolic Ca 2؉ participates in heat shock signal transduction and regulates downstream events in filamentous fungi. Our research offers a new way to understand the mechanism underlying the physiological and metabolic responses to other environmental factors in G. lucidum. This research may also provide the basis for heat shock signal transduction studies of other fungi. Ganoderma lucidum, a traditional precious medicinal mushroom, has been commonly used throughout China and Southeast Asia for many centuries as a home remedy for treating minor disorders and promoting vitality and longevity (1). Modern pharmacological and clinical research has demonstrated that G. lucidum has significant antitumor, antiviral, antihypertensive, and immunomodulatory activities (2, 3). These pharmaceutical activities come from the bioactive compounds of G. lucidum. In recent years, many of these biologically useful compounds, including ganoderic acids (GAs) and polysaccharides, have been isolated and characterized in G. lucidum (4,5). Ganoderic acids, also called triterpenoids, are one of the major secondary metabolites with pharmacological activity and are also known to be an important medicinal i...

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Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells

Cited by 91 publications

References 35 publications

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

Induction of apoptosis in MCF-7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium)

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺

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Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells

Cited by 91 publications

References 35 publications

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

Induction of apoptosis in MCF-7 human breast cancer cells by Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelium)

Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca 2+

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Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca ²⁺