Effects of Two Different Doses of Vitamin B2 and a Single Dose of Vitamin B12 Against Cyclophosphamide Induced Nephrotoxicity

Abstract

Cyclophosphamide, a medication that is used for the treatment of different types of cancer; however its' use associated with numerous adverse effects. Vitamin B2 and vitamin B12 suggested having nephroprotective effect. This work is designed to investigate the nephroprotective effect of both vitamins against cyclophosphamide induced nephrotoxicity. One hundred adult rats of both sexes were used in this study. The animals were randomly enrolled into ten groups of 10 rats each. On day eight, animals were sacrificed and blood collected for the measurement of serum superoxide dismutase 1, glutathione peroxidase; and kidney extracted for histological examination. Vitamin B2 and vitamin B12 significantly (P<0.05) increase superoxide dismutase 1, glutathione peroxidase; and the combination of vitamins produce significant (P<0.05) increase in superoxide dismutase 1 and glutathione peroxidase compared to the corresponding levels in other groups; and improve histopathological changes compared to cyclophosphamide-treated rats. In conclusion both vitamins may have nephroprotective effects against cyclophosphamide-induced nephrotoxicity.