Effects of virgin olive oil phenolics on scavenging of reactive nitrogen species and upon nitrergic neurotransmission

Puerta, R. de la; Dominguez, Montserrat; Ruı́z-Gutı́errez, Valentina; Flavill, Jenny A.; Hoult, J.R.S.

doi:10.1016/s0024-3205(01)01218-8

Cited by 148 publications

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“…In line with this, olive oil, the major fat source of Mediterranean diet, is recognized for its antioxidant properties and its positive effects on oxidative stress associated processes (Fitó et al 2007). The phenolic fraction of virgin olive oil has proved to have antioxidant activity in vitro, scavenging peroxyl radicals (Saija et al 1998), other free radicals (Gordon et al 2001) and reactive nitrogen species (De la Puerta et al 2001), or breaking peroxidative chain reactions and preventing metal ion catalyzed production of reactive oxygen species (Manna et al 1997;Mateos et al 2003). The main phenolic compounds in virgin olive oil are secoiridoid derivatives of 2-(3,4-dihydroxyphenyl)ethanol (hydroxytyrosol) (HTy) and of 2-(4-hydroxyphenyl)ethanol or tyrosol, and 2-(3,4-dihydroxyphenyl)ethyl acetate (Mateos et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Olive oil hydroxytyrosol reduces toxicity evoked by acrylamide in human Caco-2 cells by preventing oxidative stress

et al. 2011

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Humans are exposed to dietary acrylamide (AA) during their lifetime, it is therefore necessary to investigate the mechanisms associated with AA-induced toxic effects. Accumulating evidence indicates that oxidative stress contributes to AA cytotoxicity, thus, dietary antioxidants might have a protective role in colonic cells against AA toxicity. We have recently reported that hydroxytyrosol (HTy), a natural antioxidant abundant in olive oil, is able to enhance the cellular antioxidant defence capacity, thereby protecting cells from oxidative stress. In this study, we evaluate the protective role of HTy on alterations of the redox balance induced by AA in Caco-2 intestinal cells. AA cytotoxicity was counteracted by HTy by powerfully reducing ROS generation, recovering the excited enzyme antioxidant defences and decreasing phospho-Jun Kinase concentration and caspase-3 activity induced by AA. Therefore, AA-induced cytotoxicity and apoptosis are closely related to oxidative stress in Caco-2 cells and the olive oil natural dietary antioxidant HTy was able to contain AA toxicity by improving the redox status of Caco-2 cells and by partly restraining the apoptotic pathway activated by AA. Manuscript significanceThe results of the present study show that acrylamide-induced cytotoxicity and apoptosis are closely related to oxidative stress in Caco-2 cells and the olive oil natural dietary antioxidant HTy is able to decrease acrylamide toxicity by improving the cellular redox status and by partially restraining the apoptotic pathway activated by the toxin. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 Abstract Humans are exposed to dietary acrylamide (AA) during their lifetime, it is therefore necessary to investigate the mechanisms associated with AA-induced toxic effects. List of at least 4 recommended reviewers for the manuscriptAccumulating evidence indicates that oxidative stress contributes to AA cytotoxicity, thus, dietary antioxidants might have a protective role in colonic cells against AA toxicity. We have recently reported that hydroxytyrosol (HTy), a natural antioxidant abundant in olive oil, is able to enhance the cellular antioxidant defence capacity, thereby protecting cells from oxidative stress.In this study, we evaluate the protective role of HTy on alterations of the redox balance induced by AA in Caco-2 intestinal cells. AA cytotoxicity was counteracted by HTy by powerfully reducing ROS generation, recovering the excited enzyme antioxidant defences and decreasing phospho-Jun Kinase concentration and caspase-3 activity induced by AA. Therefore, AA-...

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Section: Introductionmentioning

confidence: 99%

Olive oil hydroxytyrosol reduces toxicity evoked by acrylamide in human Caco-2 cells by preventing oxidative stress

et al. 2011

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“…The treatment with this extract significantly inhibits the production of superoxide and nitric oxide, decreases lipid peroxidation and increases the activity of the superoxide dismutase, the effects being significantly higher than those from quercatin, indicating that it exercises a potent neuroprotector activity against neuronal damage in the hippocampus after global transient cerebral isquemia, which can be attributed to its antioxidant properties. The majority of studies about the effects of polyphenols from olive tree extracts indicate that their neuroprotector action is due to their antioxidant ability by eliminating reactive oxygen and nitrogen species involved in human diseases (8).…”

Section: Protection By Polyphenol Extract From Olive Stones Against Amentioning

confidence: 99%

“…All the polyphenols studied show a high antioxidant activity (7). The most abundant phenolic compound in olive tree extracts is (3.4-dihydroxyphenyl) ethanol or hydroxytyrosol: it presents scavenging activity of the superoxide radical generated by the hypoxanthina-xanthine oxidase system, and of the hydrogen peroxide generated by the reaction of guaiacol (8); it inhibits oxidation of salicylic acid by the radical -OH9; it reduces the catalase inactivation mediated by hypochlorous acid; it can act on reactive species of nitrogen like peroxynitrite (8); and it acts against linoleic acid autooxidation in lipophilic environments, as a metal chelantor -for example, iron and copper, which are essential for the organism, but can be potentially dangerous since they are capable of transferring electrons and acting as catalyzers of autooxidation reactions.…”

Section: Introductionmentioning

confidence: 99%

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Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells

Cortés‐Castell¹,

Veciana-Galindo²,

Torró-Montell³

et al. 2016

Nutr Hosp

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ResumenObjetivo: evaluar la actividad protectora del extracto de un subproducto como son los huesos de aceitunas, mediante su capacidad de inhibir la apoptosis en la línea celular humana de neuroblastoma SH-SY5Y inducida con H 2 O 2 . Material y métodos: se han cultivado 20.000 cel/pocillo, iniciando diferenciación con ácido retinoico y, una vez diferenciadas las células, se ha inducido la apoptosis con H 2 O 2 con extracto y sin presencia del mismo. Finalmente se efectúa la extracción de cDNA y el análisis de los genes proapoptótico Bax y antiapoptótico Bcl-2. La cuantificación de la expresión génica se realiza frente al gen marcador GAPDH. Resultados: la viabilidad celular con el extracto es del 97,6% (SD 5,7) con 10 mg/l y 62,8% (SD 1,2) a 50 mg/l, utilizando 10 mg/l para el ensayo de biomarcadores. Las células de la línea SH-S diferenciadas con ácido retinoico (10 µM), muestran una clara apoptosis al ser tratadas con H 2 O 2 150 µM, con una relación Bax/Bcl2 de 3,75 (SD 0,80) frente a las células diferenciadas control y sometidas a H 2 O 2 y con extracto que tienen la misma relación de 1,02 (SD 0,01-0,03). Conclusión: el extracto de huesos de aceitunas presenta una actividad antiapoptótica frente a la provocación de la muerte celular por peróxido de hidrógeno, preservando a las células de neuroblastoma humano SH-SY5Y en su estado de normalidad, al defenderlas del estrés oxidativo que produce un significativo aumento de la relación de genes apoptóticos frente a antiapoptóticos (Bax/Bcl2).Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells AbstractObjective: We evaluated the protective activity of an extract from a by-product such as olive stones, through its ability to inhibit H 2 0 2 induced apoptosis in the SH-SY5Y human neuroblastoma cell line. Material and methods: To such end, 20,000 cells/well were cultivated and differentiation with retinoic acid was initiated. Once the cells were differentiated, apoptosis was induced with and without H 2 O 2 extract. Finally, cDNA extraction was performed, and pro-apoptotic genes Bax and anti-apoptotic genes Bcl-2 were analyzed. Quantification of the gene expression was performed using the GAPDH gene marker. Results: Cell viability with the extract is 97.6% (SD 5.7) with 10 mg/l and 62.8% (SD 1.2) to 50 mg/l, using 10 mg/l for the biomarker assay. The retinoic acid differentiated SH-S cell line (10 µM) shows a clear apoptosis when treated with H 2 O 2 150 µM, with a Bax/Bcl-2 ratio of 3.75 (SD 0.80) in contrast to the differentiated control cells subjected to H 2 O 2 and with extract, which have the same ratio of 1.02 (SD 0.01-0.03). Conclusion:The olive stone extract shows anti-apoptotic activity in the provoked cell death of SH-SY5Y human neuroblastoma cells in their normal state, defending them from oxidative stress which produces a significant increase in the apoptotic gene ratio in contrast to anti-apoptotic genes (Bax/Bcl-2).

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“…Oleuropein is the most abundant of the phenolic compounds in olives [20]. It actively scavenges reactive oxygen [21] and nitrogen species [22], with anti-angiogenic activity [23].…”

Section: Introductionmentioning

confidence: 99%

Olive Leaves and Mill Waste Exert Anti-Tumoral Properties in Hepatoma Cell Line

2014

J Carcinog & Mutagen

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Different phenolic compounds such as oleuropein and hydroxytyrosol have demonstrated antitumoral activity in cancer cell lines. Olive derivatives have demonstrated beneficial properties in cardiovascular diseases. The study was addressed to show the potential beneficial properties of olive leaves and olive oil mill waste extracts in hepatoma cell lines. Aflatoxin B1 (AFB1) was administered to hepatoma cell line (HepG2). Different parameters related to cell death, cell proliferation and DNA damage were determined. The expression of p53 and c-Src (activated and inhibitory phosphorylated state) was assessed by Western blot analysis in HepG2. The olive leaves and olive oil mill waste extracts contained oleuropein and hydroxytyrosol, respectively. AFB1 induced cell proliferation and death, associated to a rise on p53 and c-Src expression in HepG2 cells. Oleuropein and hydroxytyrosol reduced cell necrosis and DNA damage in HepG2 cells. However, the administration of olive leaves and olive oil mill waste extracts increased cell necrosis, DNA damage. These effects of natural extracts were associated with a reduction of activated c-Src expression and cell proliferation in AFB1-treated HepG2 cells. These results support that olive leaves extract and olive oil mill waste extracts, but not oleuropein and hydroxytyrosol, may exert antitumoral effect against a hepatoma cancer cell line.

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Effects of virgin olive oil phenolics on scavenging of reactive nitrogen species and upon nitrergic neurotransmission

Cited by 148 publications

References 0 publications

Olive oil hydroxytyrosol reduces toxicity evoked by acrylamide in human Caco-2 cells by preventing oxidative stress

Olive oil hydroxytyrosol reduces toxicity evoked by acrylamide in human Caco-2 cells by preventing oxidative stress

Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells

Olive Leaves and Mill Waste Exert Anti-Tumoral Properties in Hepatoma Cell Line

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