Effects of vitamin E supplementation in the extender on frozen-thawed bovine semen preservation

Hu, Jianhong; Zhao, X.-L.; Tian, Wanqiang; Liu, Zan; Li, Q.-W.

doi:10.1017/s1751731110001679

Cited by 36 publications

(17 citation statements)

References 39 publications

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“…Recent studies demonstrated that the change of temperature could influence bull sperm quality significantly during cryopreservation (Hu et al 2011). In this study, in which bull samples were frozen and thawed, the results suggested that motility and membrane integrity of bull sperm gradually decreased following the process of freezing-thawing.…”

Section: Discussionmentioning

confidence: 62%

“…Frozen-thawed bull semen plays an important role in this procedure. However, there is about 40-50% loss in bull semen quality during the freezing-thawing process, including sperm motility, effective survival time, and antioxidant enzymes activity (Hu et al 2011). Various extenders and freezing procedures have been described in bull semen to minimize detrimental effects of cryopreservation on sperm.…”

Section: Introductionmentioning

confidence: 99%

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Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Zhang¹,

Hong²,

Yan³

et al. 2015

Czech J. Anim. Sci.

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ABSTRACT:Heat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bulls by artificial vagina. Sperm motility and plasma membrane integrity of the semen samples at three stages (fresh, after equilibration, and frozen-thawed) were evaluated. The HSP70 expression level at the three stages was detected using real-time PCR. The results indicated that HSP70 expression level, membrane integrity, and sperm motility in the fresh semen were higher than those of the sperm after equilibration and freezing-thawing (P < 0.05), the HSP70 expression level, plasma membrane integrity, and sperm motility in sperm after equilibration were higher than those of the frozenthawed sperm (P < 0.05). The correlation between HSP70 expression level and sperm motility was positive (ranging from 0.327 to 0.785). The results suggest that HSP70 expression level in bull spermatozoa was gradually decreased following the process of freezing-thawing, and might be associated with bull sperm motility.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Zhang¹,

Hong²,

Yan³

et al. 2015

Czech J. Anim. Sci.

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“…Vitamin E is the main component of the antioxidative system of sperm cells, one of the first protective membranes against lipid peroxidation (Hu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Brezezińska-Slebodzińska et al (1995) presented that over short periods of incubation, vitamin E and GSH protects against lipid peroxidation reducing losses in the semen quality. Additional studies have shown that Trolox protects cryopreserved sperm decreasing lipid peroxidation (Minaei et al, 2012) and bovines (Hu et al, 2011), reducing motility losses. The beneficial effect has also demonstrated in cryopreserved swine semen (Varo-Ghiuru et al, 2015;Jeong et al, 2009;Peña et al, 2004;2003).…”

Section: Introductionmentioning

confidence: 99%

Oxidative Status of Boar Semen during Storage

Zanella¹,

Zanella²,

Poetini³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.

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“…ROS has a negative effect on cryopreserved spermatozoa function (Wiechetek & Slaweta, 1987;Gadea et al, 2004;Roca et al, 2005;Bucak et al, 2008;Shoae & Zamiri, 2008;SarIözkan et al, 2009;Bansal & Bilaspuri, 2011;Cocchia et al, 2011;Hu et al, 2011;Ashrafi et al, 2013). It has been suggested that the application of antioxidants such as cysteine, taurine, alpha-tocopherol (vitamin E), superoxide dismutase, catalase, glutathione, butylated hydroxytoluene and melatonin into the semen media can have beneficial effects on decreasing ROS production.…”

Section: Discussionmentioning

confidence: 99%

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

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The present study determined the effects of the addition of rosemary extract (ROM), glutathione (GSH), and their combination (ROM + GSH) to freezing extender on the quality of bull semen after cryopreservation. Before cryoperservation, the samples were diluted in a tris-egg yolk (TEY) extender containing 5 mM GSH (treatment I), 5 or 10 g L–1 ROM (treatments II and III), and ROM with GSH (5 mM GSH with 5 or 10 g L–1 of ROM) (treatments IV and V). An extender containing no antioxidants (non-ROM/GSH-treated) served as control group. Kinematic parameters were evaluated by means of a computer-assisted semen analysis (CASA). The viability and membrane integrity of the sperm were assessed using eosin-nigrosin stain and the hypo-osmotic swelling test (HOST) at 0 and 2 h after freezethawing. Lipooxidative parameters, superoxide dismutase, and glutathione peroxidase (GPx) activity were assessed after thawing. Treatment III showed positive effects for total motility (TM) (p < 0.01), average path velocity (VAP) (p < 0.001), viability (p < 0.01) and HOST (p < 0.01); however, lipid peroxidation (LPO) decreased (p < 0.05) and GPx activity increased (p < 0.05) immediately after thawing compared to the control. The TM (p < 0.01), VAP (p < 0.01), viability (p < 0.01), HOST (p < 0.01) decreased in LPO (p < 0.01) and GPx activity (p < 0.05) for treatment V and the viability and GPx activity (p < 0.05) for treatment I were significantly higher than for the control group at 2 h after thawing. It was concluded that the inclusion of ROM and its combination with GSH improves the post-thaw quality of bull semen.

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Effects of vitamin E supplementation in the extender on frozen-thawed bovine semen preservation

Cited by 36 publications

References 39 publications

Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Association of heat shock protein 70 with motility of frozen-thawed sperm in bulls

Oxidative Status of Boar Semen during Storage

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

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