Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress

Abstract

The aim of the present study was to evaluate the effect of β-glucan on acute hypoxia-induced oxidative stress and the changes in energy metabolism by determining ROS production, activities and mRNA levels of energy metabolism enzyme (PK, F-ATPase, SDH and MDH), and in gene expression of HIF-1α in the liver of large yellow croaker. Fish were injected with β-glucan at a dose of 0 or 5 mg kg(-1) body weight on 6, 4 and 2 days before exposed to 1.5 and 7.0 mg DO L(-1) for 48 h. The results showed that β-glucan enhanced survival rate and reduced ROS during the lethal hypoxic stress, indicating that β-glucan could ameliorate hypoxia-induced oxidative stress. Obtained results also showed that β-glucan could up-regulate activities and mRNA levels of PK, demonstrating that β-glucan increased anaerobic glycolysis capacity. Furthermore, a coordinated transcriptional regulation of energy metabolism enzyme genes was observed, suggesting that HIF-1α is required for regulating these genes. In conclusion, β-glucan could alleviate cute hypoxia-induced oxidative stress in large yellow croker by enhancing anaerobic glycolysis capacity, emphasizing a central role of transcription factor HIF-1α in the process.

Efficacy of Glucans on Growth Variables, Antioxidant Potential, and Immunity of Growing Rabbits

“…All of these antioxidant tools are contributed in scavenging reactive oxygen species (ROS), the excessive exposure of which causes oxidative stress in the host's cells, and then produce a lot of MDAm, which is one of the most important lipid-oxidation products and an important indicator of peroxidative tissue injury (Janero, 1990). A substantial reduction in MDA was seen in the fishfed BG diet, which agrees with our findings (Kim et al, 2009;Zeng et al, 2016;Zhu and Wu, 2018). corollary, the current data could point to BG supplementation having a positive impact on liver function and tissue peroxidation.…”

Effect of β glucan dietary levels on immune response and hematology of channel catfish Ictalurus punctatus juveniles

“…Fish cage culture is a highly efficient intensive system that is a potentially stressful environment due to overcrowding, inducing fish susceptibility to pathogen infection due to immune system alterations, which may result in fish disease (Villamil et al, 2003). In recent years, immunostimulants have been included in the feed of cultured fish in order to improve their immune system (Meena et al, 2013;De et al, 2014;Vaseeharan & Thaya, 2014;Yang et al, 2016), stress resistance, antioxidant activity (Zhao et al, 2012;Guzmán-Villanueva et al, 2014;Zanuzzo et al, 2015;Zeng et al, 2016), inflammatory response (Boltaña et al, 2011), and resistance to bacterial (Bonaldo et al, 2009;Meshram et al, 2015), virus (Parmar et al, 2012) and parasite infections (Jaafar et al, 2011). Glucans are polysaccharides with different primary molecular structures that vary depending on their source, which have different implications on their immunostimulant activity (Akramienė et al, 2007); glucans are commonly found ___________________ Corresponding editor: Jesús Ponce-Palafox in mushrooms, algae, yeast, cereals and cell walls of bacteria (Ringø et al, 2011;Meena et al, 2013).…”

The potential benefits of dietary β‐glucan against growth retardation, immunosuppression, oxidative stress and expression of related genes and susceptibility to Aeromonas hydrophila challenge in Oreochromis niloticus induced by herbicide pendimethalin

“…Where the rise in MDA level in PMN group, could clarified due to PMN‐induced production of enormous free radicals that develops the hepatic oxidative procedure, further provokes a decrease in the commotions of antioxidant enzyme catalysts by stopping the manufacture of the enzymes (Hamed & El‐Sayed, 2019). Supplementation with βG adjusted the lifted level of MDA in tilapia exposed to PMN protect tilapias from lipid peroxidation and correction in SOD and CAT (Dietrich‐Muszalska et al., 2011; Zeng et al., 2016). Gene expression of the SOD and CAT conform to these observations.…”