Effectus synthetica analogum activa centrum GM-CSF-peptide ZP2 in anti-cytokine actio Сandidae fungi et facultates ad producere cytokine-sicut substantiae

Abstract

The synthetic analogue of the active center of GM-CSF — peptide ZP2 has a wide range of biological effects. However, its effect on the biological properties of Candida has not been previously investigated. The aim is to analyze the nature of the effect of synthetic peptide ZP2 on anti–cytokine activity (ACA) and the ability to produce cytokine-like substances (CLS) in Candida. Materials and methods. 26 vaginal strains of Candida spp. were used in the experiment. from conditionally healthy pregnant women. To study the effect of the ZP2 peptide on the ACA of Candida and their CLS production, Candida were cultured in Saburo broth with the ZP2 peptide at 37oC for 24 hours. The ZP2 peptide was not added to the control. ACA for IL-8, TNFα and IL17A and CLS products were determined by the ELISA method according to a well-known technique in the original modification using kits of Cytokin LLC (St. Petersburg). To assess the ACA of Candida, the proportion of cytokine inactivation in the experiment relative to the control was calculated and expressed in pg/ml; the production of CLS by Candida was judged by the level of cytokines in the experiment and control, expressed in pg/ml. The data were subjected to statistical processing. Results and discussion. The multidirectional effect of the peptide ZP2 on the ACA of Candida with respect to a number of cytokines and their ability to produce individual CLS has been established. The ZP2 peptide did not change the incidence of ACA against IL8 in Candida spp. strains, but increased the severity of this trait in C. non-albicans isolates. The ZP2 peptide inhibited ACA against TNFα in C. albicans, but stimulated the manifestation of ACA against IL17A only in C. non-albicans. The ZP2 peptide slightly increased the proportion of C. albicans with the ability to produce substances similar to IL8 and TNFα without significantly changing the severity of the trait. Among C. non-albicans, the number of strains producing these substances did not change significantly under the action of the ZP2 peptide, but the expression of the trait increased. The ZP2 peptide reduced the proportion of Candida spp. strains capable of producing IL17A-like substances. Conclusion. Candida are able to secrete compounds that inactivate various cytokines, as well as produce cytokine-like substances. The synthetic analogue of the active center of GM-CSF-peptide ZP2 has a modifying effect on the manifestation of these properties by fungi.