Effet de la temperature sur la structure cristalline d'un biocorail

Irigaray, J.L.; Oudadesse, H.; Fadl, H. El; Sauvage, Thierry; Thomas, Gérard; Vernay, Anne-Marie

doi:10.1007/bf02235441

Cited by 28 publications

(17 citation statements)

References 1 publication

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“…Similar results have been observed on other materials made of biocompatible calcium phosphate materials. 26,27 The amount of radioactivity incorporation in the cells was higher in coral than in calcite materials. This could be due to increased cell attachment on the coral material more so than on the calcite although the porosity was approximately the same, but this result could also represent a higher rate of DNA syntheins.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of proliferation and protein expression of human bone marrow cells cultured on coral crystallized in the aragonite or calcite form

Fricain

Bareille

Ulysse

et al. 1998

J. Biomed. Mater. Res.

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The two crystalline forms of CaCO3, aragonite (from natural coral) and calcite (from natural limestone), have been used with success as bone graft substitutes. However, natural coral transformed into calcite by heating has never been tested. The objective of this work was to study the proliferation and alkaline phosphatase, osteonectin, and osteocalcin expression of human bone marrow cells cultured on CaCO3 crystallized both in the aragonite form (natural coral) and in the calcite form (natural coral modified by heating). The methods used to characterize calcite obtained from the coral were volumic porosimetry, scanning electron microscopy (SEM) and X-ray diffraction. Cell colonization of the material was assessed by SEM performed on days 1, 7, 20, and 30 and [3H]thymidine incorporation was performed on days 3, 7, 12, 18, 25, and 32. Phenotypic expression was assessed by using in situ cytochemistry (alkaline phosphatase), immunocytochemistry (osteonectin and osteocalcin), and hybridization (osteocalcin, beta-actin, and alkaline phosphatase mRNA). Results showed the transformation of aragonite into calcite after heating, the conservation of macroporosity, and a modification of the surface. Calcite appeared to have a smoother and more uniform surface than aragonite crystals. As for [3H]thymidine there was an increase incorporation from days 3 to 18, a stabilization from days 18 to 25, and a decrease from days 25 to 32. After 20 days of culture, immunological studies using monoclonal antibodies to osteocalcin, osteonectin, cytochemical analysis of alkaline phosphatase activity, and in situ hybridization using osteocalcin, beta-actin, and alkaline phosphatase cDNA indicated that the cells had not lost their osteoblastic phenotype. These experiments demonstrate that coral crystallized in the aragonite or calcite form present a similar degree of specific cytocompatibility.

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Section: Discussionmentioning

confidence: 96%

Evaluation of proliferation and protein expression of human bone marrow cells cultured on coral crystallized in the aragonite or calcite form

Fricain

Bareille

Ulysse

et al. 1998

J. Biomed. Mater. Res.

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“…All pores intercommunicated (open porosity). Coral implants were sterilized by autoclaving, which does not affect the coral composition 32 .…”

Section: Experimental Protocolmentioning

confidence: 99%

Tissue-engineered bone regeneration

et al. 2000

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Bone lesions above a critical size become scarred rather than regenerated, leading to nonunion. We have attempted to obtain a greater degree of regeneration by using a resorbable scaffold with regeneration-competent cells to recreate an embryonic environment in injured adult tissues, and thus improve clinical outcome. We have used a combination of a coral scaffold with in vitro-expanded marrow stromal cells (MSC) to increase osteogenesis more than that obtained with the scaffold alone or the scaffold plus fresh bone marrow. The efficiency of the various combinations was assessed in a large segmental defect model in sheep. The tissue-engineered artificial bone underwent morphogenesis leading to complete recorticalization and the formation of a medullary canal with mature lamellar cortical bone in the most favorable cases. Clinical union never occurred when the defects were left empty or filled with the scaffold alone. In contrast, clinical union was obtained in three out of seven operated limbs when the defects were filled with the tissue-engineered bone.

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“…The hES-MPs were derived from an undifferentiated hESC line (SA002.5), and the derivation and characterization of hES-MPs has been described earlier. 33,34 hES-MPs were expanded in DMEM-HG, supplemented with the same additives as described above for the hMSCs.…”

Section: Cell Typesmentioning

confidence: 99%

“…Scaffolds were steam autoclaved at 115°C for 20 min, which was shown not to affect the composition. 34 Then, 330 coral scaffolds were washed three times (30 min) with Dulbecco's Modified Eagle Medium-High Glucose (DMEM-HG; PAA Laboratories) supplemented with 1% penicillin-streptomycin-amphotericin (PAA Laboratories). To remove air bubble entrapped within the material, samples were finally vacuum treated and incubated overnight at 37°C in 5% CO 2 .…”

Section: Coral Scaffoldsmentioning

confidence: 99%

Human Embryonic Stem Cell-Derived Mesodermal Progenitors Display Substantially Increased Tissue Formation Compared to Human Mesenchymal Stem Cells Under Dynamic Culture Conditions in a Packed Bed/Column Bioreactor

Peppo

Sladkova

Sjövall

et al. 2013

Tissue Engineering Part A

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Bone tissue engineering represents a promising strategy to obviate bone deficiencies, allowing the ex vivo construction of bone substitutes with unprecedented potential in the clinical practice. Considering that in the human body cells are constantly stimulated by chemical and mechanical stimuli, the use of bioreactor is emerging as an essential factor for providing the proper environment for the reproducible and large-scale production of the engineered substitutes. Human mesenchymal stem cells (hMSCs) are experimentally relevant cells but, regardless the encouraging results reported after culture under dynamic conditions in bioreactors, show important limitations for tissue engineering applications, especially considering their limited proliferative potential, loss of functionality following protracted expansion, and decline in cellular fitness associated with aging. On the other hand, we previously demonstrated that human embryonic stem cell-derived mesodermal progenitors (hES-MPs) hold great potential to provide a homogenous and unlimited source of cells for bone engineering applications. Based on prior scientific evidence using different types of stem cells, in the present study we hypothesized that dynamic culture of hES-MPs in a packed bed/column bioreactor had the potential to affect proliferation, expression of genes involved in osteogenic differentiation, and matrix mineralization, therefore resulting in increased bone-like tissue formation. The reported findings suggest that hES-MPs constitute a suitable alternative cell source to hMSCs and hold great potential for the construction of bone substitutes for tissue engineering applications in clinical settings.

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Effet de la temperature sur la structure cristalline d'un biocorail

Cited by 28 publications

References 1 publication

Evaluation of proliferation and protein expression of human bone marrow cells cultured on coral crystallized in the aragonite or calcite form

Evaluation of proliferation and protein expression of human bone marrow cells cultured on coral crystallized in the aragonite or calcite form

Tissue-engineered bone regeneration

Human Embryonic Stem Cell-Derived Mesodermal Progenitors Display Substantially Increased Tissue Formation Compared to Human Mesenchymal Stem Cells Under Dynamic Culture Conditions in a Packed Bed/Column Bioreactor

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