Effets de l'ecdystérone sur la morphogenèse de la patte imaginale de Tenebrio molitor au cours du stade prénymphal en culture in vitro

Lenoir-Rousseaux, Jean-Jacques; Huet, Cl.

doi:10.1016/0016-6480(75)90044-1

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…These cells make leg cuticle during each larval moult but rapidly grow to make most of the pupal leg at metamorphosis. Interestingly, the beetle, Tenebrio molitor , presents a situation in which there appears not to be an organized imaginal primordium [31]. Rather, like the abdomen, its leg appears to undergo a fine-grained cellular transformation from larva to adult.…”

Section: The Evolution Of the Pupal Stage And Of Imaginal Discsmentioning

confidence: 99%

The evolution of insect metamorphosis: a developmental and endocrine view

Truman

Riddiford

2019

Phil. Trans. R. Soc. B

142

116

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Developmental, genetic and endocrine data from diverse taxa provide insight into the evolution of insect metamorphosis. We equate the larva–pupa–adult of the Holometabola to the pronymph–nymph–adult of hemimetabolous insects. The hemimetabolous pronymph is a cryptic embryonic stage with unique endocrinology and behavioural modifications that probably served as preadaptations for the larva. It develops in the absence of juvenile hormone (JH) as embryonic primordia undergo patterning and morphogenesis, the processes that were arrested for the evolution of the larva. Embryonic JH then drives tissue differentiation and nymph formation. Experimental treatment of pronymphs with JH terminates patterning and induces differentiation, mimicking the processes that occurred during the evolution of the larva. Unpatterned portions of primordia persist in the larva, becoming imaginal discs that form pupal and adult structures. Key transcription factors are associated with the holometabolous life stages: Krüppel-homolog 1 ( Kr-h1 ) in the larva, broad in the pupa and E93 in the adult. Kr-h1 mediates JH action and is found whenever JH acts, while the other two genes direct the formation of their corresponding stages. In hemimetabolous forms, the pronymph has low Broad expression, followed by Broad expression through the nymphal moults, then a switch to E93 to form the adult. This article is part of the theme issue ‘The evolution of complete metamorphosis’.

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Section: The Evolution Of the Pupal Stage And Of Imaginal Discsmentioning

confidence: 99%

The evolution of insect metamorphosis: a developmental and endocrine view

Truman

Riddiford

2019

Phil. Trans. R. Soc. B

142

116

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Tissue Culture Methods

Oberlander¹

1980

Springer Series in Experimental Entomology

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The in vitro development of the pupal integument and the effects of ecdysteroids in Tenebrio molitor (Insecta, Coleoptera)

Quennedey

Delbecque

et al. 1983

Cell Tissue Res.

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In order to study the pupal-adult metamorphosis of Tenebrio in vitro, pupal sternites of different ages were cultured in Landureau's medium and their development systematically observed by electron microscopy. In hormone-free medium, explants taken from young pupae do not secrete pupal postecdysial cuticle in vitro, and the epidermis spontaneously detaches from the pupal cuticle. On the contrary, explants taken from pharate adults continue to secrete adult preecdysial cuticle in vitro, and the epidermis never detaches from the cuticle. Ecdysterone in physiological concentrations (0.2 to 4 micrograms/ml) induces the secretion of a new cuticle in explants from young pupae but the epidermis remains undifferentiated. Ecdysone is necessary for the induction of some adult differentiation. Moreover, the quality of the cuticle secreted in vitro is increased by the addition of 2% foetal calf serum; the best results have thus far been obtained in a medium containing 0.2 microgram/ml ecdysone, 1 microgram/ml ecdysterone, and 2% foetal calf serum.

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Effets de l'ecdystérone sur la morphogenèse de la patte imaginale de Tenebrio molitor au cours du stade prénymphal en culture in vitro

Cited by 6 publications

References 13 publications

The evolution of insect metamorphosis: a developmental and endocrine view

The evolution of insect metamorphosis: a developmental and endocrine view

Tissue Culture Methods

The in vitro development of the pupal integument and the effects of ecdysteroids in Tenebrio molitor (Insecta, Coleoptera)

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