Efficacy of a commercially available post-thaw bovine semen sexing kit in both single-ovulating and hyperstimulated cows

Curry, Erin; Pratt, Scott L.; Lapin, D.; Gibbons, J.

doi:10.1016/j.anireprosci.2009.01.012

Cited by 4 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, no statistically significant difference was found in pregnancy rates between the HP group [52.0% (26/50)] and corresponding control [56.0% (28/50)], and between the BP group [64.0% (32/50)] and corresponding control [58.0% (29/50)]. These results are in agreement with the findings of Curry et al (2009).…”

Section: Discussionsupporting

confidence: 83%

“…However, there have been important differences between the results of the manufacturer (Williams 2007) and the findings of several studies, as well as between the studies regarding the efficacy of the HP (Gerard et al 2008;Curry et al 2009) kit on the sex ratio. Although the in vitro efficacy of the BP kit has been reported in conference proceedings, there has as of yet been no in vivo study published in any scientific journal regarding the efficacy of the BP kit, according to our knowledge.…”

Section: Discussionmentioning

confidence: 99%

“…However, Curry et al (2009) have reported that the treatment of frozen-thawed semen with HP did not affect the number of embryos collected (4.76 in HP group, 3.55 in control group) in ovarian hyperstimulated, or the pregnancy rates (54.5% in HP group, 48.0% in control group) in single-ovulating cows. Besides, the manufacturer has also claimed that these kits provide an increase in the pregnancy rates by at least at 5-20%.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the company further claims that BP enhances the male calf ratio in a relatively similar process, but that the male sperm is stimulated instead of the female sperm resulting in more bull calves then heifers and greatly increasing the revenue generated from bull calf sales (Reidhead 2007). To the best of our knowledge, only a single study has been published in a scientific journal (Curry et al 2009) along with two conference proceedings (Gerard et al 2008;Sassone et al 2009) regarding the efficacy of HP, while for the efficacy of BP, only a single in vitro study has been presented at a conference (Gerard et al 2008). The results of the few studies are controversial regarding the effects of the HP kit on the sex ratio (Gerard et al 2008;Curry et al 2009).…”

mentioning

confidence: 99%

“…To the best of our knowledge, only a single study has been published in a scientific journal (Curry et al 2009) along with two conference proceedings (Gerard et al 2008;Sassone et al 2009) regarding the efficacy of HP, while for the efficacy of BP, only a single in vitro study has been presented at a conference (Gerard et al 2008). The results of the few studies are controversial regarding the effects of the HP kit on the sex ratio (Gerard et al 2008;Curry et al 2009). Further, there is no in vivo study published in any scientific journal about the efficacy of the BP kit.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows

Demirci¹,

Yüksel²,

Sönmez³

et al. 2015

Vet. Med. - Czech

View full text Add to dashboard Cite

It has been claimed that artificial insemination (AI) of cows with frozen-thawed semen treated with commercially produced kits, Heiferplus TM (HP, in favour of female gender) and Bullplus TM (BP, in favour of male gender), increases the birth chance of calves with desired sex ratio by at least 20-25% and pregnancy rates by at least 5-20%. Hence, this study was conducted to investigate the efficacy of HP and BP kits as combined with AI on the pregnancy rates, foetal sex ratios and some reproductive parameters in cows. For this, a total of 200 cows (100 Holsteins and 100 Simmentals) from three to five years old were used. Fifty Holstein and 50 Simmental cows served as controls. The other half of Holstein and Simmental cows was artificially inseminated with frozen-thawed semen treated with HP and BP, respectively. Findings showed that the AI of cows with frozen-thawed semen treated with HP had no significant effect on the pregnancy rate [52.0% (26/50) in HP group; 56.0% (28/50) in control group], female calf ratio [52.0% (13/25) in HP group; 44.4% (12/27) in control group], embryonic death, abortion, stillbirth, twinning and gestation length as compared to the control group. Similarly, AI of cows with frozen-thawed semen treated with BP did not lead to any significant effect on the pregnancy rate [64.0% (32/50) in BP group; 58.0% (29/50) in control group], male calf ratio [53.1% (17/32) in BP group; 39.3% (11/28) in control group] and other reproductive parameters as compared to the control group. In conclusion, HP and BP treatments of semen used in the AI provided only slight, non-significant increases in female (7.6%) and male (13.8%) calf ratios, respectively. Recently, sex pre-selection before fertilisation has been attempted in the field of reproductive biotechnology. The sex of foetuses is determined by whether a sperm, which performs fertilisation, carries an X or Y chromosome (Seidel 1999;Seidel and Johnson 1999). The optimal method for determining foetal sex is to separate X and Y chromosome-bearing spermatozoa before fertilisation, and to inseminate the females with the desired (sexed) semen portion. For this purpose, X and Y chromosome-bearing spermatozoa can be separated with a 85-90% success rate using flow cytometric sorting. However, the disadvantages of this method include sperm damage due to the separation procedure, increased embryonic death rate due to the usage of DNA dyes and the high financial cost of the method, which has limited its worldwide availability in field conditions (Seidel 2007).It has been claimed that insemination of cows with frozen-thawed bull semen treated with Heiferplus TM (HP) and Bullplus TM (BP) kits, marketed by a commercial company, increases the birth chance of calves with desired sex ratio by at least 20-25% and the pregnancy rate by at least 5-20%. Although the ingredients are undisclosed, the manufacturer, quoted on the internet, cites HP efficacy in both ovarian hyperstimulated and naturally ovulating cattle (Williams 2007). According to the manufac-

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows

Demirci¹,

Yüksel²,

Sönmez³

et al. 2015

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

Sex selection techniques and their implications

Naniwa

Uchiyama

2021

CABI Reviews

View full text Add to dashboard Cite

Sex selection techniques provide economic benefits to dairy and beef herd management. Therefore, the development of such techniques has attracted the attention of reproductive biologists. There have been numerous studies concerning the development of sex selection techniques. As the sex of the offspring is determined by certain chromosomes, namely the X and Y chromosomes in mammals, most studies have focused on sperm sexing, attempting to separate the X and Y chromosome-bearing sperm based on differences in deoxyribonucleic acid (DNA) content, head size/volume, motility, and immunological specificity. However, most of these methods have failed to show reproducibility. Only the flow cytometric method has been confirmed to be accurate and reliable thus far. More than three decades have passed since this technique was first developed. The sexed semen produced with the method is currently commercialized and widely used for artificial insemination (AI) in cattle around the world. Recently, however, another technique based on differences in DNA contents using a fluidics device was developed by a commercial company. Studies focused on immunological approaches and the modification of sperm motility have also described the successful separation of the two types of sperm. Therefore, the present review evaluated the available sex selection techniques and their implications.

show abstract

Sex Ratio of Embryos from Hyperstimulated Beef Cattle.

Davis

2009

Biology of Reproduction

View full text Add to dashboard Cite

Efficacy of a commercially available post-thaw bovine semen sexing kit in both single-ovulating and hyperstimulated cows

Cited by 4 publications

References 10 publications

Effects of semen sexing kits (Heiferplus<sup>TM</sup> and Bullplus<sup>TM</sup>) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows

Effects of semen sexing kits (Heiferplus<sup>TM</sup> and Bullplus<sup>TM</sup>) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows

Sex selection techniques and their implications

Sex Ratio of Embryos from Hyperstimulated Beef Cattle.

Contact Info

Product

Resources

About