Efficacy of an Oral Solution Prepared from the Ultrasonic Extract of Radix dichroae roots against Eimeria tenella in Broiler Chickens

Abstract

This study was conducted to determine the optimal dose of the oral solution of the ultrasonic extract of Radix dichroae (UERD) and to provide experimental support for a safe clinical dose for anticoccidial treatment of broiler chickens. Radix dichroae root extracts were prepared using the ultrasonic extraction method. The anticoccidial activity of the oral solution prepared from the ultrasonic extract of Radix dichroae roots was tested in broiler chickens following oral infection with a field isolate of E. tenella. Ninety Lingnan yellow broiler chickens (14 days old) were randomly divided into nine groups (n = 10), including six UERD oral solution treatments (0.25, 0.50, 1.50, 2.50, 3.50, and 5.00%), a toltrazuril group (0.10%), an E. tenella-infected control group, and a healthy control group. All groups were inoculated orally with 7 × 104 sporulated E. tenella oocysts (Guangdong strain) except for the healthy control group. The chickens in the seven drug-treated groups were administered a UERD oral solution or toltrazuril in drinking water for 7 days. The anticoccidial efficacy of the UERD oral solution was evaluated by the bloody diarrhoea severity level, relative body weight gain (rBWG), lesion score, oocyst per gram (OPG), and anticoccidial index (ACI). Compared with the infected control group, there were no significant differences in the groups treated with UERD oral solution or toltrazuril with regard to the lesion changes in the caecal regions (P>0.05); however, the blood contents, OPG, and oocyst score in three UERD oral solution treatment groups (0.50, 1.50, and 2.50%) were significantly reduced, and the bloody diarrhoea was also alleviated. The ACI in three UERD oral solution treatment groups (0.50%, ACI = 143.7; 1.50%, ACI = 151.0; and 2.50%, ACI = 144.3) was higher than that in the toltrazuril group (ACI = 127.0), and the rBWG in the 1.50% UERD oral solution treatment group (95.0%) was similar to that in the healthy control group (100%), which was also 12.5% higher than that in the toltrazuril group (82.5%). The findings of this study demonstrated that the UERD oral solution (0.50% ～ 2.50% dose range) showed better prevention, anticoccidial efficacy, and growth promotion effects than toltrazuril (0.10%), and the 1.50% dose level of UERD oral solution in water is the clinically recommended dose according to the present study conditions.

Comparative effect of papaya (Carica papaya) leaves' extract and Toltrazuril on growth performance, hematological parameter, and protozoal load in Sonali chickens infected by mixed Eimeria spp.

“…Similarly, Hirani et al [ 37 ] reported that the acute phase of infection had decreased the level of Hb in the infected groups and the values also returned to usual after recovery. Coccidiosis results dysfunction of spleen, leading to acute massive hemorrhage manifested by bloody diarrhea [ 38 ], which might be one of the reasons for a decreased Hb level.…”

Anticoccidial Activity of Qinghao Powder Against Eimeria tenella in Broiler Chickens

“…There are seven Eimeria species affecting chickens, including Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. The parasites multiply in the intestinal epithelia, destroying the cells and reducing digestive capacity and nutrient absorption in the bird, with Eimeria tenella being highly pathogenic and causing caecal coccidiosis (1)(2)(3)(4)(5). Coccidiosis is responsible for 6-10% of all broiler mortalities, and the annual loss caused by Eimeria infection to the poultry industry is estimated at more than $3 billion (6,7).…”