Efficacy of cell free supernatant from Bacillus subtilis ET-1, an Iturin A producer strain, on biocontrol of green and gray mold

Ambrico, A.; Trupo, Mario

doi:10.1016/j.postharvbio.2017.08.001

Cited by 56 publications

(28 citation statements)

References 39 publications

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“…Thus, the MICs in vitro of iturin A (µg mL -1 ) were as follows: 4.1 for M. laxa, 8.5 for M. fructicola, P. italicum and P. expansum and 16.9 for B. cinerea and P. digitatum (Table 4). These MICs in vitro are in partial agreement with those of Ambrico and Trupo (2017) who determined a MIC of iturin A of 6 and 3 µg mL -1 for P. digitatum and B. cinerea, respectively, in a solid medium in vitro test. These discrepancies could be explained by several factors such as the different in vitro test…”

Section: Correlation Between Iturin a Concentration And Antifungal Acsupporting

confidence: 88%

“…subtilis GA1 in a culture medium optimized for lipopeptide production (named Opt), Touré et al (2004) obtained concentrations of fengycins, iturins and surfactins of 520, 460 and µg•mL -1 , respectively. Also, Ambrico and Trupo (2017) in High Medium Broth (HMB) achieved the highest amount of iturin, mg L -1 , from B. subtilis ET-1 after 50 h of incubation. Thus, the incubation time is an essential factor in the production of secondary metabolites together with the culture medium.…”

Section: Lipopeptide Production Curvementioning

confidence: 99%

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The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots

et al. 2019

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The aim of this work was to elucidate the role of the secondary metabolites produced by B. amyloliquefaciens BUZ-14 against B. cinerea, M. fructicola, M. laxa, P. digitatum, P. italicum and P. expansum both in vitro and in planta. The entire cell free supernatant (CFS) and the lipopeptide fraction (LPF) showed similar antifungal activities, completely inhibiting all the fungi at dilutions of 1:24 or even lower, whereas the nonbutanolic fraction (NBF) barely inhibited the fungi. However, when the LPF and CFS were applied on fruit, only brown rot in peaches and blue rot in apples was totally inhibited. The main families of metabolites in the LPF were iturin A, fengycin and surfactin with maximum concentrations of 407, 853 and 658 µg mL-1 , respectively. Subsequently, a TLC-bioautography revealed iturin A as the key metabolite in the inhibitions and allowed us to establish in vivo MICs of 16.9 and 33.9 µg mL-1 for Monilinia species and P. expansum, respectively. The application of 24 hold BUZ-14 cultures supressed brown rot in peaches and also blue rot in apples but failed to inhibit the other diseases. However, BUZ-14 was only able to grow and produce iturin A in peaches so we can deduce that the amount of iturin A brought with the cultures (36 ± 14 µg mL-1) could be enough to control both diseases. The strong antifungal activity of the iturin A present in the BUZ-14 CFS suggests that it could be successfully used for postharvest disease control. However, future research is necessary to maximize the iturin A production by B. amyloliquefaciens BUZ-14 in order to optimize a commercial application.

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Section: Correlation Between Iturin a Concentration And Antifungal Acsupporting

confidence: 88%

Section: Lipopeptide Production Curvementioning

confidence: 99%

The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots

et al. 2019

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“…Waewthongrak et al (2015) showed that the cyclolipopeptides secreted by the B. subtilis strain ABS-S14 elicit a broad-spectrum antibiotic effect, reducing the number of Penicillium strains exposed to B. subtilis and cell-free cultivation by 96.2% and 90.9%, respectively. Ambrico and Trupo (2017) further confirmed that Iturin A produced by B. subtilis ET-1 can effectively inhibit the occurrence of blue mold on lemon fruit and gray mold on strawberry fruit. Ahmad et al (2017) isolated and identified a B. subtilis strain 330-2 from rapeseed; this strain was found to secrete indole-3-acetic acid, iron carrier, lysozyme, organic and inorganic phosphates from different sources, and zinc ions.…”

Section: Introductionmentioning

confidence: 92%

Volatile organic compounds (VOCs) from Bacillus subtilis CF-3 reduce anthracnose and elicit active defense responses in harvested litchi fruits

Zhao

et al. 2019

AMB Expr

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In this study, we investigated the effects of volatile organic compounds (VOCs) produced by Bacillus subtilis CF-3 on the growth and development of Colletotrichum gloeosporioides and evaluated the elicitation of active defense responses in harvested litchi fruits. In vitro experiments were conducted to explore the bacteriostatic effect of VOCs in inhibiting pathogenic fungi by means of plate enthalpy test, scanning electron microscopy, transmission electron microscopy, and gas chromatography–mass spectrometry (GC–MS). The results showed that 2,4-di- tert -butylphenol and CF-3 24-h fermentation broth (24hFB) can significantly inhibit the germination of fungal spores, disrupt hyphal and cell morphology, and decrease cell membrane fluidity and integrity, resulting in the changes of indexes. In addition, the bacteriostasis of VOCs in the defensive ability of litchi fruits to C. gloeosporioides was studied, and it was shown that 2,4-di- tert -butylphenol and CF-3 24hFB can inhibit the activity of the pathogenic enzymes (pectinase and cellulase) secreted by C. gloeosporioides to reduce the decomposition of plant tissues, activate antioxidant enzymes (peroxidase, polyphenol oxidase, catalase, and superoxide dismutase) in the fruit to eliminate excessive reactive oxygen species in fruits in order to reduce plant cell damage and activate disease resistance enzymes (phenylalanineammonialyase, chitinases, β-1,3-glucanase) to enhance the resistance of litchi fruits to C. gloeosporioides and inhibit its growth. This study investigated the bacteriostasis of VOCs in inhibiting C. gloeosporioides and inducing the resistance of litchi fruits, providing a theoretical basis for future applications.

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“…These lipopeptides show strong activity against fungal pathogens like Penicillium , Pestalotia , Gloesporidium , Colletotrichum , Alternaria , Botrytis etc. (Falardeau et al ; Ambrico and Truppo ). Lipopeptides of Fengycin group can also damage the cells through membrane destabilization and are reported to show activities against pathogens like Fusarium , Rhizoctonia , Monilinia , etc.…”

Section: Antimicrobial Metabolitesmentioning

confidence: 99%

Bacillusspecies in soil as a natural resource for plant health and nutrition

et al. 2019

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The genus Bacillus is one of the predominant bacterial genera found in soil, and several species of this genus have been reported from diverse ecological niches. Endowed with tremendous genetic and metabolic diversity, Bacillus spp. serve multiple ecological functions in soil ecosystem from nutrient cycling to conferring stress tolerance to plants. Members of the genus Bacillus are known to have multiple beneficial traits which help the plants directly or indirectly through acquisition of nutrients, overall improvement in growth by production of phytohormones, protection from pathogens and other abiotic stressors. This functionally versatile genus is one of the most commercially exploited bacteria in the agro-biotechnology industry. Still its potential has not been realized sufficiently and requires an emphasis towards translating the relevant technologies from laboratory to land for the benefit of mankind.

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Efficacy of cell free supernatant from Bacillus subtilis ET-1, an Iturin A producer strain, on biocontrol of green and gray mold

Cited by 56 publications

References 39 publications

The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots

The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots

Volatile organic compounds (VOCs) from Bacillus subtilis CF-3 reduce anthracnose and elicit active defense responses in harvested litchi fruits

Bacillusspecies in soil as a natural resource for plant health and nutrition

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