Efficacy of cephalosporins against enterobacteria isolated from patients with chronic osteomyelitis

Шипицына, И. В.; Осипова, Елена Владимировна

doi:10.51620/0869-2084-2022-67-3-158-162

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…K. pneumoniae is one of the most frequently isolated gram-negative agents in cases of osteomyelitis. In studies conducted on osteomyelitis, cephalosporin resistance is around 80% in K. pneumonia strains [ 25 ]. Carbapenems remain a good treatment option for the Enterobacteriaceae family that produces extended-spectrum beta-lactamase.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Therapeutic Efficacy of Antibiotic-Loaded Polymeric Tissue Scaffold and Bone Cement in the Regeneration of Infected Bone Tissue

Konya,

Yilmaz

et al. 2023

Cureus

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Background Local antibiotic applications have been used in chronic osteomyelitis and have been defined as an adjunctive treatment method. Biodegradable materials are also used for the same purpose by adding antibiotics. The fact that it does not require additional surgery to be removed is an important advantage. In this study, we intended to develop a new biodegradable drug-loaded polymeric scaffold with good antibiotic release and compare the microbiological results with antibiotic-impregnated bone cement. Methodology A tissue scaffold containing poly(2-hydroxyethyl methacrylate) (PHEMA) was prepared in our laboratory and loaded with ertapenem and daptomycin antibiotics. The surface morphology and pore geometries of drug-loaded and unloaded scaffolds were analyzed by a scanning electron microscope under vacuum. The dose-dependent antiproliferative effects of PHEMA scaffold, drug-loaded scaffold, cement, and drug-loaded cement on osteoblast cells were investigated. To evaluate drug release kinetics, the absorbance values of the scaffold loaded with ertapenem and daptomycin were measured with the spectrometer. For microbiological tests, ertapenem and daptomycin-impregnated cement and scaffold, as well as the control scaffold and cement samples, were investigated for their antibacterial activities on Staphylococcus aureus and Klebsiella pneumoniae strains using the disc diffusion method. These microorganisms are one of the most common microorganisms in osteomyelitis. Results The efficacy of antibiotic-impregnated scaffold and cement on both gram-negative and gram-positive microorganisms was investigated. The daptomycin zone diameter in S. aureus ATCC 29233 strain was 17 mm, whereas it was 24 mm for scaffold and 22 mm for cement. Scaffold was found to be more effective than cement against S. aureus strain. The K. pneumoniae ATCC BAA-2814 strain was found to be resistant to ertapenem, but the zone diameter was 21 mm for scaffold and 20 mm for cement. Ertapenem-loaded scaffold was found to be more effective than cement. It was found that the antimicrobial activity of the scaffold was higher than cement. When we evaluated the release profiles, for the daptomycin-loaded cement group, 98% of daptomycin was cumulatively released within 30 minutes, and for the daptomycin-loaded scaffold group, 100% of daptomycin was cumulatively released in six days. To compare ertapenem-loaded cement and scaffold, 98% of ertapenem was cumulatively released within 10 minutes in the cement group. For the scaffold group, 100% of ertapenem was cumulatively released in 17 days. We found that the scaffold released the antibiotic more slowly and for a longer duration. Therefore, it was thought that the scaffold would be more effective on biofilm and the treatment of osteomyelitis would be more successful. Conclusions The produced scaffold was compared...

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Section: Discussionmentioning

confidence: 99%

Comparison of the Therapeutic Efficacy of Antibiotic-Loaded Polymeric Tissue Scaffold and Bone Cement in the Regeneration of Infected Bone Tissue

Konya,

Yilmaz

et al. 2023

Cureus

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“…Klebsiella pneumoniaeis one of the most frequently isolated gram negative agents in cases of osteomyelitis. In studies conducted in osteomyelitis, cephalosporin resistance is around 80% in K. pneumonia strains [25]. Carbapenems remain a good treatment option for the Enterobacteriaceae family that produces extended-spectrum beta-lactamase.…”

Section: Sambri Et Al Developed a Bioeliminable Scaffold Consisting O...mentioning

confidence: 99%

Comparison of the therapeutic efficacy of antibiotic-loaded polymeric tissue scaffold and bone cement in the regeneration of infected bone tissue

Konya,

Yılmaz

et al. 2023

Preprint

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Osteomyelitis is a progressive disease characterized by damage to bone tissue by microorganisms through infectious and inflammatory processes. Local antibiotic applications have been used in chronic osteomyelitis and have been defined as an adjunctive treatment method.Biodegradable materials are also used for the same purpose by adding antibiotics. The fact that it does not require additional surgery to be removed is an important advantage. The current study is therefore intended to develop a biodegradable drug-loaded polymeric scaffold with good antibiotic release that can be an alternative to antibiotic-impregnated bone cement. Methods Tissue scaffold containing poly (2-hydroxyethyl methacrylate) (PHEMA) was prepared in our laboratory and loaded with Ertapenem and Daptomycin antibiotics. The surface morphology and pore geometries of drug-loaded and unloaded scaffolds were analyzed by SEM (scanning electron microscope) under vacuum. The dose-dependent antiproliferative effects of PHEMA scaffold, drug-loaded scaffold, cement, and drug-loaded cement on osteoblast cells were investigated. To evaluate drug release kinetics, the absorbance values of the scaffold loaded with Ertapenem and Daptomycin were measured with the spectrometer. For microbiological tests, Ertapenem and Daptomycin impregnated cement and scaffold, as well as the control scaffold and cement samples were investigated for their antibacterial activities on Staphylococcus aureusand Klebsiella pneumoniae strains using the disc diffusion method. Results The efficacy of antibiotic-impregnated scaffold and cement on both gram negative and gram positive microorganisms was investigated. Daptomycin zone diameter in S. aureus ATCC strain was 17 mm, whereas this value was 24 mm for scaffold and 22 mm for cement. Scaffold was found to be more effective than cement against S. aureus strain. On K. pneumoniae ATCC strain, it was found that this strain was resistant to Ertapenem, but the zone diameter was 21 mm for scaffold and 20 mm for cement.Ertapenem-loaded scaffold was found to be more effective than cement. It was found that the antimicrobial activity of the scaffold was higher than cement. When we evaluated the release profiles, for the Daptomycin-loaded cement group, 98%of Daptomycin was cumulatively released within 30 min and for the Daptomycin-loaded scaffold group, 100%of Daptomycin was cumulatively released in 6 days. To compare Ertapenem-loaded cement and scaffold, 98% of Ertapenem was cumulatively released within 10 min in the cement group. For the scaffold group, 100% of Ertapenem was cumulatively released in 17 days. We found that the scaffold released the antibiotic more slowly and during a longer duration. Therefore, it was thought that scaffold would be more effective on biofilm and treatment of osteomyelitis would be more successful. Conclusions The scaffold produced was compared with cement, and it was concluded that the scaffold has better release and antimicrobial efficacy.Scaffold is more advantageous than cement because it is bioeliminable. Thus, there will be no need for a second surgical intervention and possible mortality and morbidities will be prevented. Because of all these features, scaffold seems promising in the local treatment of osteomyelitis.

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“…This pathology is characterized by a polymorphism of clinical manifestations, associated not so much with the epidemic or epizootic situation, but with the age and state of the patient's defenses. Opportunistic enterobacteria, under certain conditions, also play an active role in the etiology of acute intestinal infections in humans and animals [5,6,7,8,12].…”

Section: Introductionmentioning

confidence: 99%

Persistence factors and antibiotic susceptibility of enterobacteria isolated from various animal species

Ermakov

Titov²

2022

BIO Web Conf.

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During the study, we isolated 575 strains of enterobacteria from various animal species belonging to the genus Escherichia, Shigella, Salmonella, Klebsiella, Proteus, Providencia, Hafnia, Morganella, Enterobacter, Citrobacter, Serratia, Erwinia, Kluyvera, Yersinia. In farm animals, the amount of transient pathogenic enterobacteria Salmonella enteritidis and Yersinia enterocolitica did not exceed 0.22-0.12%. Among wild animals, the proportion of pathogenic Salmonella enteritidis and Yersinia enterocolitica did not exceed 0.24-0.11%, while Shigella dysenteriae and Shigella flexneri were isolated at a low concentration of 0.01%. In the feces of zoo animals, the amount of Salmonella enteritidis and Yersinia enterocolitica did not exceed 0.21-0.10%, while Shigella dysenteriae and Shigella flexneri were detected at the level of 0.01% of the total concentration of enterobacteria. The share of Salmonella enteritidis and Yersinia enterocolitica in domestic animals did not exceed 0.012-0.04%. Persistence factors in Salmonella enteritidis, Shigella dysenteriae and Shigella flexneri, Klebsiella oxytoca, and Yersinia enterocolitica were observed to be the highest among all isolated enterobacteria. Enterobacteria showed high resistance to benzylpenicillin from the group of natural penicillins to streptomycin, cephalothin from the group of cephalosporins of the first generation, polymyxin B, ofloxacin (tarivid), and metronidazole. Carbenicillin from the group of carboxypenicillins and piperacillin from the group of ureidopenicillins, kanamycin, amikacin, and gentamicin, cefepime from the group of IV generation cephalosporins, tetracycline, doxycycline and chloramphenicol, nalidixic acid, trimethoprim showed the highest antimicrobial activity against all cultures of enterobacteria isolated in this study.

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Efficacy of cephalosporins against enterobacteria isolated from patients with chronic osteomyelitis

Cited by 3 publications

References 11 publications

Comparison of the Therapeutic Efficacy of Antibiotic-Loaded Polymeric Tissue Scaffold and Bone Cement in the Regeneration of Infected Bone Tissue

Comparison of the Therapeutic Efficacy of Antibiotic-Loaded Polymeric Tissue Scaffold and Bone Cement in the Regeneration of Infected Bone Tissue

Comparison of the therapeutic efficacy of antibiotic-loaded polymeric tissue scaffold and bone cement in the regeneration of infected bone tissue

Persistence factors and antibiotic susceptibility of enterobacteria isolated from various animal species

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