Efficacy of Entomopathogenic Trichoderma Isolates against Sugarcane Woolly Aphid, Ceratovacuna lanigera Zehntner (Hemiptera: Aphididae)

Abstract

Ceratovacuna lanigera Zehntner (Hemiptera: Aphididae) is a destructive insect pest of sugarcane that is responsible for reducing the quality, yield, and sugar content. Ecofriendly methods of pest control, such as the use of biological control, is encouraged since it does not exhibit the harmful effects that are usually seen when using chemical-based pesticides. Here, we evaluated the efficacy of several entomopathogenic fungi isolates against C. lanigera under laboratory conditions. A total of seven isolates were obtained from agricultural soils that were collected from various locations from Sabah, Malaysia, using the insect bait method. Among the seven, four isolates (TMS114, TMS623, TMS628, and TMS707) were positively identified as T. harzianum (Hypocreales: Hypocreaceae), two isolates (TMS45, TMS321) as T. asperellum (Hypocreales: Hypocreaceae), and one isolate (TMS633) as T. hamatum (Hypocreales: Hypocreaceae) based on a combination of morphological characteristics and molecular data using ITS 1-5.8 S-ITS 2 regions of the rDNA. Four different concentrations (1 × 105, 1 × 106, 1 × 107, and 1 × 108 mL−1) of conidia suspensions of the isolates were applied separately on nymphs and adults of C. lanigera. The results showed that mortality in both nymphs and adults was dependent on the conidia concentration and exposure time. Among the four concentrations, the highest mortality of C. lanigera was found to range from 59.99 to 75.70% for nymphs after 72 h and from 57.45 to 72.31% for adults after 6 days of treatments at the concentration of 1 × 108 conidia mL−1. Trichodermaharzianum (TMS623) showed significantly highest mortality of 75.70% for nymphs and 72.31% for adults among the seven isolates. . However, the nymph and adult mortality rates for the isolates were 70.00% & 68.00% for TMS114, 66.42% & 63.10% for TMS628, 67.85% & 65.24% for TMS707, 61.42% & 58.80% for TMS45, 59.99% & 57.45% for TMS321 and 63.56% & 60.91% for TMS633, respectively. For nymph mortality, T. harzianum (TMS623) showed a LC50 (lethal concentration) value of 6.30 × 105 conidia mL−1 and LC90 value of 3.01 × 109 conidia mL−1, respectively, and for adult mortality, the values were found of 6.16 × 105 conidia mL−1 for LC50 and 2.23 × 1010 conidia mL−1 for LC90, respectively. The lethal time (LT) values for nymph and adult mortality were found of 42.65 h and 3.89 days for LT50 and 93.32 h and 8.70 days for LT90 by T. harzianum (TMS623). Therefore, T. harzianum (TMS623) showed more pathogenic potential against C. lanigera and can be further applied to determine its efficacy under field conditions. To our best knowledge, this is the first report of T.harzianum,T.hamatum, and T.asperellum species that are used as entomopathogenic fungi against sugarcane woolly aphid, C. lanigera.

Identification of native soil-derived Trichoderma spp. isolates and analysis of their antagonist traits against Lasiodiplodia theobromae causing stem-end rot in papaya

“…In the present study, the obtained results enabled the identification and separation of isolates in three different taxonomic groups: T. asperellum (BRS-1, BRS-3 and BRS-4), T. harzianum (BRS-2, BRS-5, BRS-6, BRS-7 and BRS-8), and T. koningiopsis (BRS-9). These findings are closed to those obtained by others authors, including Islam et al (2021),who identified 7 isolates of Trichoderma spp. obtained from agricultural soils collected from Sabah, Malaysia, using a combination of morphological and molecular data from the ITS 1-5.8S-ITS 2 regions of the rDNA.…”

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth

“…Further examination indicated that there was an intense parasitic growth of the T. gamsii hyphae across the lower abdomen and respiratory ostiole of the Delia radicum larva [175]. Other than that, T. harzianum, T. asperellum, and T. hamatum showed pathogenic potential against Cetarovacuna lanigera, a destructive insect pest of sugarcane that is responsible for reducing the quality, yield, and sugar content [176].…”

Effects of Trichoderma harzianum Strain T22 on the Arthropod Community Associated with Tomato Plants and on the Crop Performance in an Experimental Field

“…Laboratory studies showed that Trichoderma spp. negatively influence both piercing-sucking [ 26 , 33 , 34 , 35 , 72 ] and chewing insects [ 27 , 33 ]. In addition, Trichoderma spp.…”