Efficacy of freeze-dried platelet-rich plasma in bone engineering

Nakatani, Yuya; Agata, Hideki; Sumita, Yoshinori; Koga, Takanori; Asahina, Izumi

doi:10.1016/j.archoralbio.2016.10.006

Cited by 35 publications

(27 citation statements)

References 25 publications

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“…An initial review of the literature highlighted the great variety of platelet-derived products and the heterogeneity in their nomenclature and processing methods, with confusion over their therapeutic properties and suggested applications [18][19][20][21]. Most protocols of PRP preparation are very similar to each other: the withdrawal of autologous blood in the presence of an anticoagulant; its centrifugation with plasma separation; leukocyte inclusion or exclusion; and the activation of platelet with different stimuli.…”

Section: Discussionmentioning

confidence: 99%

The Number of Platelets in Patient’s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP

Bosetti

Boffano

Marchetti

et al. 2019

IJMS

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The objectives of this study were to compare platelet-rich plasma (PRP) from patients with different concentrations of platelets and to assess the influence of these PRP preparations on human osteoblast (hOB) activity. In the literature, growth factors released by activated platelets have been considered responsible for the active role of PRP on bone regeneration but no specific role has been attributed to lysophosphatidic acid (LPA) as a possible effector of biological responses. In this study, patients were grouped into either group A (poor in platelets) or group B (rich in platelets). Clots from PRP fraction 2 (F2-clots), obtained with CaCl2 activation of PRP from the two groups, were compared macroscopically and microscopically and for their mechanical properties before testing their activity on the proliferation and migration of hOB. LPA was quantified before and after PRP fractioning and activation. The fibrin network of F2-clots from patients with a lower platelet concentration had an organized structure with large and distinct fibers while F2-clots from patients in group B revealed a similar structure to those in group A but with a slight increase in density. ELISA results showed a significantly higher plasma level of LPA in patients with a higher platelet concentration (group B) in comparison to those in group A (p < 0.05). This different concentration was evidenced in PRP but not in the clots. Depending on the number of platelets in patient’s blood, a PRP-clot with higher or lower mechanical properties can be obtained. The higher level of LPA in PRP from patients richer in platelets should be considered as responsible for the higher hOB activity in bone regeneration.

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Section: Discussionmentioning

confidence: 99%

The Number of Platelets in Patient’s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP

Bosetti

Boffano

Marchetti

et al. 2019

IJMS

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“…A recent study showed that compared with the traditional PRP, freeze-dried PRP retained comparatively more growth factors after storage for four weeks at room temperature [ 54 ]. Another study showed that compared with traditional PRP, freeze-dried PRP released more growth factors, such as PDGF-BB and TGF-b1, and induced more bone formation in vivo [ 55 ]; these features could further improve the advantages of the freeze-dried PRP.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of 3D-Printed Polycaprolactone Scaffolds Coated with Freeze-Dried Platelet-Rich Plasma for Bone Regeneration

Chen

Wei

et al. 2017

Materials

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Three-dimensional printing is one of the most promising techniques for the manufacturing of scaffolds for bone tissue engineering. However, a pure scaffold is limited by its biological properties. Platelet-rich plasma (PRP) has been shown to have the potential to improve the osteogenic effect. In this study, we improved the biological properties of scaffolds by coating 3D-printed polycaprolactone (PCL) scaffolds with freeze-dried and traditionally prepared PRP, and we evaluated these scaffolds through in vitro and in vivo experiments. In vitro, we evaluated the interaction between dental pulp stem cells (DPSCs) and the scaffolds by measuring cell proliferation, alkaline phosphatase (ALP) activity, and osteogenic differentiation. The results showed that freeze-dried PRP significantly enhanced ALP activity and the mRNA expression levels of osteogenic genes (ALP, RUNX2 (runt-related gene-2), OCN (osteocalcin), OPN (osteopontin)) of DPSCs (p < 0.05). In vivo, 5 mm calvarial defects were created, and the PRP-PCL scaffolds were implanted. The data showed that compared with traditional PRP-PCL scaffolds or bare PCL scaffolds, the freeze-dried PRP-PCL scaffolds induced significantly greater bone formation (p < 0.05). All these data suggest that coating 3D-printed PCL scaffolds with freeze-dried PRP can promote greater osteogenic differentiation of DPSCs and induce more bone formation, which may have great potential in future clinical applications.

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“…In this way, to leverage the potential of lyophilized plasma products in implantology, FD-PRF was proven to enhance the expression of transcription factors related to alveolar bone regeneration in mice compared to traditional PRF [63] (Table 3). Moreover, the equivalence between fresh-frozen PRP and FD-PRP was shown in terms of PDGF-BB, TGF-β and VEGF concentrations and stimulation of bone formation in immunocompromised mice [64]. However, in an apparent contradiction, rabbit BMSCs differentiation and proliferation was better in CaCl 2 -activated fresh PRP compared with FD-PRP [65].…”

Section: Dentistry Researchmentioning

confidence: 98%

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

Andı́a

Perez‐Valle

Amo

et al. 2020

IJMS

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The complex biology of platelets and their involvement in tissue repair and inflammation have inspired the development of platelet-rich plasma (PRP) therapies for a broad array of medical needs. However, clinical advances are hampered by the fact that PRP products, doses and treatment protocols are far from being standardized. Freeze-drying PRP (FD-PRP) preserves platelet function, cytokine concentration and functionality, and has been proposed as a consistent method for product standardization and fabrication of an off-the-shelf product with improved stability and readiness for future uses. Here, we present the current state of experimental and clinical FD-PRP research in the different medical areas in which PRP has potential to meet prevailing medical needs. A systematic search, according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analyses) guidelines, showed that research is mostly focused on wound healing, i.e., developing combination products for ulcer management. Injectable hydrogels are investigated for lumbar fusion and knee conditions. In dentistry, combination products permit slow kinetics of growth factor release and functionalized membranes for guided bone regeneration.

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Efficacy of freeze-dried platelet-rich plasma in bone engineering

Cited by 35 publications

References 25 publications

The Number of Platelets in Patient’s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP

The Number of Platelets in Patient’s Blood Influences the Mechanical and Morphological Properties of PRP-Clot and Lysophosphatidic Acid Quantity in PRP

Evaluation of 3D-Printed Polycaprolactone Scaffolds Coated with Freeze-Dried Platelet-Rich Plasma for Bone Regeneration

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

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