Efficacy of leukocyte‐ and platelet‐rich plasma gel (L‐PRP gel) in treating osteomyelitis in a rabbit model

Abstract

Leukocyte-and platelet-rich plasma gel (L-PRP gel), a new autologous product which was previously utilized in several surgical procedures to enhance tissue healing, is now increasingly used as a promising treatment method for infections. In this study, we investigated the antibacterial property of L-PRP gel against Methicillin-resistive Staphylococcus aureus (MRSA, ATCC 43300) in a rabbit model of osteomyelitis. Tibial osteomyelitis was induced in 40 New Zealand white rabbits using the MRSA strain. Three weeks after induction, the rabbits with tibial osteomyelitis were randomly divided into four groups: Control group (no treatment); Van group (debridement and parenteral treatment with vancomycin alone); L-PRP gel þ Van group (debridement and local L-PRP gel injection, plus parenteral treatment with vancomycin); L-PRP gel group (debridement and local L-PRP gel injection). All rabbits were sacrificed 6 weeks after debridement. The antibacterial efficacy was evaluated by radiological, microbiological, and histological examinations. Newly formed bone was also quantified. The best therapeutic efficacy, including infection elimination and bone defect repair, was observed in the L-PRP gel þ Van group. Although not comparable to vancomycin, L-PRP gel also exibited antimicrobial efficacy in vivo. We believe that a combination of L-PRP gel and antibiotics could be a favorable alternative for the treatment of osteomyelitis.

The Cheapest and Easiest Way to Make Platelet-rich Plasma Preparation

“…Improvement of functional recovery and analgesic requirement was reported [10,11]. Li et al [12] reported that L-PRP had a statistically significant antimicrobial effect on MRSA infected rabbit.…”

The “biological chamber” method – use of autologous platelet-rich plasma (PRP) in the treatment of poorly healing lower-leg ulcers of venous origin

“…The high concentration of platelets in the plasma guarantees delivery of higher than physiological concentrations of growth factors to the damaged site, which can improve the conditions of tissue regeneration (4,6). In addition, the leukocytes present in the preparation increase its antibacterial properties (7,8).…”

The Effect of Local Platelet Rich Plasma Therapy on the Composition of Bacterial Flora in Chronic Venous Leg Ulcer

“…In vitro studies showed good efficacy of PRP against S. aureus (MSSA), which was comparable to the one of gentamycin and oxacillin (Cieślik Bielecka et al, 2012). Li et al (2013) observed a significant antibacte rial activity of PRP against MRSA in experimentally induced osteitis in rats. Also Moojen et al (2008) con firmed the efficacy of PRP against S. aureus.…”