Efficacy of RNA interference using nanocarrier‐based transdermal dsRNA delivery system in the woolly apple aphid, <i>Eriosoma lanigerum</i>

Guo, Youguang; Fan, Yinjun; Teng, Ziwen; Wang, Lingyun; Tan, Xi; FangHao, Wan; Zhou, Hongxu

doi:10.1002/arch.21888

Cited by 9 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The RNAi efficiency of acetylcholinesterase ( AChE ) gene silencing in P. xylostella can be improved by concatemerised dsRNAs (C‐dsRNA) (Sharath et al., 2019). Nanocarriers can increase the RNAi efficiency of the V‐ATPase subunit D ( ATPD ) gene of Woolly apple aphid from 40.5% to 98.5% (Guo et al., 2022). Since the genes with lower transcript abundance required less dsRNA for silencing (Zhu & Palli, 2020).…”

Section: Discussionmentioning

confidence: 99%

Functions of the sulfatase‐modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera

Zhang,

Zhou,

Cai

et al. 2024

J Applied Entomology

View full text Add to dashboard Cite

The system of gulcosinoalte sulfatase–sulfatase‐modifying factors (Sumfs) in herbivorous insects fulfils an essential function in defending host plant glucosinolates (GLSs)—the compounds of cruciferous plants—by swiftly desulfurising them. Previously, eight HaSulfs and one HaSumf1 were identified in Helicoverpa armigera. GLSs have a significant impact on the growth and development of H. armigera larvae. They can induce the expression of HaSulf in the midgut of H. armigera at both gene and protein levels. In the present study, we utilised RNAi technology to silence the HaSumf1 gene and explored its potential impact on the growth, development, and adaptation of GLSs. The sequence analysis has indicated that the HaSumf1 gene in H. armigera shares a high degree of sequence identity with other insect genes and is expressed at its highest level in the fifth‐instar phase. Additionally, the qRT‐PCR results reveal that the expression of HaSumf1 was upregulated by 1.26 and 0.07 times in third‐instar and 1.65 and 0.19 times in fifth‐instar when fed with 0.004% and 0.04% GLS, respectively. The effectiveness of dsSumf1 silencing was observed in both third‐ and fifth‐instar stages, with silencing rates of 65.5%–88.9% and 61.3%–74.6%, respectively (p < 0.05). The findings of the bioassay indicate that H. armigera's body weight at fifth‐instar significantly increased after treatment with 0.004% GLS and 0.04% GLS, while the larval survival rate, pupation rate, and pupa weight decreased. Notably, when feeding dsRNA‐encapsulated carbon quantum dots (CQD‐dsRNA), this trend was significantly strengthened (p < 0.05). Taken together, these findings provide evidence supporting HaSumf1's conservation involvement in the adaptation of host plant GLSs during lepidopteran insect evolution. Therefore, it plays a critical role in the growth and development of H. armigera, offering molecular targets for developing novel pesticides and targeted control measures against this vital pest.

show abstract

Section: Discussionmentioning

confidence: 99%

Functions of the sulfatase‐modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera

Zhang,

Zhou,

Cai

et al. 2024

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…A number of studies have evaluated the insecticidal potential of targeting H + V-ATPase subunits in several insect species, including a variety of insect pests, showing strong negative impacts on growth and survival ( Ahmed, 2016 ; Sato et al, 2019 ; Rahmani and Bandani, 2021 ; Guo et al, 2022 ; Liu et al, 2022 ; Shi et al, 2022 ; Zeng et al, 2022 ). So far, the majority of these studies focused on targeting the different subunits of the V 1 domain of these proton pumps.…”

Section: Discussionmentioning

confidence: 99%

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust

Van Lommel,

Holtof,

Tilleman

et al. 2023

Front. Physiol.

View full text Add to dashboard Cite

The digestive tract constitutes an important interface between an animal’s internal and external environment. In insects, available gut transcriptome studies are mostly exploratory or look at changes upon infection or upon exposure to xenobiotics, mainly performed in species belonging to holometabolan orders, such as Diptera, Lepidoptera or Coleoptera. By contrast, studies focusing on gene expression changes after food uptake and during digestion are underrepresented. We have therefore compared the gene expression profiles in the midgut of the desert locust, Schistocerca gregaria, between three different time points after feeding, i.e., 24 h (no active digestion), 10 min (the initial stage of feeding), and 2 h (active food digestion). The observed gene expression profiles were consistent with the polyphagous herbivorous lifestyle of this hemimetabolan (orthopteran) species. Our study reveals the upregulation of 576 genes 2 h post-feeding. These are mostly predicted to be associated with digestive physiology, such as genes encoding putative digestive enzymes or nutrient transporters, as well as genes putatively involved in immunity or in xenobiotic metabolism. The 10 min time point represented an intermediate condition, suggesting that the S. gregaria midgut can react rapidly at the transcriptional level to the presence of food. Additionally, our study demonstrated the critical importance of two transcripts that exhibited a significant upregulation 2 h post-feeding: the vacuolar-type H(+)-ATPAase and the sterol transporter Niemann-Pick 1b protein, which upon RNAi-induced knockdown resulted in a marked increase in mortality. Their vital role and accessibility via the midgut lumen may make the encoded proteins promising insecticidal target candidates, considering that the desert locust is infamous for its huge migrating swarms that can devastate the agricultural production in large areas of Northern Africa, the Middle East, and South Asia. In conclusion, the transcriptome datasets presented here will provide a useful and promising resource for studying the midgut physiology of S. gregaria, a socio-economically important pest species.

show abstract

“…Another study also investigated the RNAi efficacy of the ATPD gene in woolly apple aphids (Eriosoma lanigerum) via a nanocarrier-mediated transdermal dsRNA delivery system. Their results suggested that the interference efficiency was greatly increased using nanocarriers and induced high aphid mortality [86]. The nanocarrier-delivered RNAi method was also used to silence the flightin, vestigial (vg), and Ultrabithorax (Ubx) genes, which suppressed the wing development in M. persicae [85,91].…”

Section: Other Delivery-method-mediated Gene Silencing For Aphid Controlmentioning

confidence: 99%

RNA-Interference-Mediated Aphid Control in Crop Plants: A Review

Zhang

Zhong

et al. 2022

Agriculture

View full text Add to dashboard Cite

Crop plants suffer severe yield losses due to the significant damages caused by aphids. RNA interference (RNAi) technology is a versatile and environmentally friendly method for pest management in crop protection. Transgenic plants expressing siRNA/dsRNA and non-transformative methods such as spraying, microinjection, feeding, and a nanocarrier-delivery-mediated RNAi approach have been successfully applied for agricultural insect pest management. In this review, we summarize the application of host-induced gene silencing (HIGS)-mediated RNAi, spray-induced gene silencing (SIGS)-mediated RNAi, and other delivery-method-mediated RNAi methods for aphid control. We further discuss the challenges in RNAi application and propose potential solutions to enhance RNAi efficiency.

show abstract

Efficacy of RNA interference using nanocarrier‐based transdermal dsRNA delivery system in the woolly apple aphid, Eriosoma lanigerum

Cited by 9 publications

References 36 publications

Functions of the sulfatase‐modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera

Functions of the sulfatase‐modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust

RNA-Interference-Mediated Aphid Control in Crop Plants: A Review

Contact Info

Product

Resources

About