Poultry meat production and consumption face several challenges under economic, social and environmental perspectives, and increasing concerns are associated with food loss and waste minimization. One of the main issues is related to the absence of a homogeneous and standardized separate collection of bio-waste at country level, which makes chicken bones, skin and food waste valorization a challenging goal. The present research, implementing the material flow analysis to the Italian poultry sector, aims at measuring poultry-related co-products and by-products, exploring food waste, chicken bones and skin and the current trends in the Italian bio-waste separate collection. Then, it discusses alternative separate collection strategies and sustainable consumption habits. Data have been collected according to a research triangulation approach, whereas system boundaries consider slaughterhouse, distribution and final consumption stage. It emerges that more than 1.50 Mt of live animals have been processed to obtain 0.46 Mt of fresh meat and 0.76 Mt of co-products and by-products, of which more than 0.32 Mt are represented by chicken bones and skin. In addition, more than 0.15 Mt of food waste have been recorded. The research adds an extra step towards the identification of awareness campaigns and separate collection strategies at national level.