Efficiency of Cryotherapy in Avoiding Myringotomy Closure in Rats

Chirteş, Felician; Trombitaș, Veronica; Vlad, Diana; Tabaran, Flaviu; Berce, Cristian; Albu, Silviu

doi:10.1007/s12070-014-0782-7

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…For environmental enrichment, autoclaved braided cotton dental rolls were used (Celluron ® ; Hartmann, Heidenhelm, Germany), as previously described. 15,16 The study was designed and performed in accordance with the ARRIVE Guidelines for Reporting Animal Research. 17 All animal-handling procedures were performed according to the European and Romanian guidelines.…”

Section: Animals and Housingmentioning

confidence: 99%

In vivo assessment of bone marrow toxicity by gold nanoparticle-based bioconjugates in Crl:CD1(ICR) mice

Berce¹,

Lucan²,

Petrushev

et al. 2016

IJN

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Introduction The present study aimed at evaluating the biodistribution of Tween ® 20-gold nanoparticle (GNP) conjugates and their potential toxicity on the bone marrow before moving on to Phase I clinical trials. Materials and methods Tween ® 20-conjugated GNPs were injected intravenously for 21 days in male Crl:CD1(ICR) mice. Body weight of the mice was evaluated each day. After the sub-chronic Tween ® 20-GNPs administration, blood samples were harvested, and a full blood count was done individually. Total Au quantity from all major organs was assessed using inductively coupled plasma mass spectrometry. One femur and the sternum obtained from each animal were used for histological assessment. Results Our data showed that the Tween ® 20-GNP conjugates were found in large quantities in the bladder. Au was shown to accumulate in the hematopoietic bone tissue, with significant side effects such as leucopoiesis and megakaryopoiesis. The mice had a higher white blood cell and platelet count as opposed to the control group. This suggested that the previously described leukopenic effects of isoflurane were overridden by the leucopoietic effects of Tween ® 20-GNPs. Conclusion It was uncertain whether the mice were reactive to Au as it is a foreign substance to the tissues or whether the side effects observed were a precursor condition of a more severe hematological condition. Au was found to be hepatotoxic, urging the need for further studies in order to achieve better in vivo compliance and exploit the immense potential of GNPs in cancer pharmacology.

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Section: Animals and Housingmentioning

confidence: 99%

In vivo assessment of bone marrow toxicity by gold nanoparticle-based bioconjugates in Crl:CD1(ICR) mice

Berce¹,

Lucan²,

Petrushev

et al. 2016

IJN

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“…Cryotherapy, a tool for dermatologists and gastroenterologists for many years, has received increasing attention among otolaryngologists. The usefulness of this method has been demonstrated in experimental animal studies in maintaining maxillary antro-meatal and myringotomy permeability in rat and rabbit experimental models [ 2 , 5 , 7 , 8 , 9 ]. It was demonstrated as safe, well-tolerated and efficacious in endoscopical application to the middle meatus used to freeze the posterior nasal nerve in chronic rhinitis.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Spray Cryotherapy on Lesion-Induced Osteitis in a New Murine Experimental Model

et al. 2023

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Background and Objectives: Endoscopic sinus surgery is considered the gold management strategy for difficult-to-treat chronic rhinosinusitis. The inflammatory bony process is incriminated as being involved in the unfavorable evolution and recurrence of the disease. Osteitis is significantly increased in patients that have been previously submitted to surgery, and it is more often present in patients with extended radiological disease and in patients undergoing revision surgery. The aim of the research is to demonstrate the presence of inflammations and neo-osteogenesis associated with nasal mucosal surgical injury and the correlation between their severity and to evaluate the efficacy of low-pressure spray cryotherapy in reducing inflammation and bone remodeling. Materials and Methods: The experimental murine model was conducted over a period of 80 days and included a total of 60 adult female Wistar rats, with three periods of withdrawal of 20 individuals each from the experiment. After inducing a bilateral mechanical injury by brushing, low-pressure spray cryotherapy application was performed unilaterally, and tissue samples were prepared specifically for histological analysis. The scores for inflammation and osteitis were compared over time and between the two nasal fossae. Results: Osteitis and inflammation were induced by a simple mucosal brushing lesion, similar to surgical injury. We identified the presence of inflammation in 95% of the specimens, and it was present over time. Moreover, criteria for bone remodeling were clearly highlighted in a percentage of 72% of the specimens. There was a direct correlation between the severity of inflammation and neo-osteogenesis, with a statistical significance of p = 0.050. Low-pressure spray cryotherapy was safe and effective in reducing inflammation (p = 0.020) and osteitis (p = 0.000) with a safety profile. Conclusions: Low-pressure cryotherapy reduces the severity of mucosal inflammation and osteitis in lesion-induced neo-osteogenesis.

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Cutaneous wound healing using polymeric surgical dressings based on chitosan, sodium hyaluronate and resveratrol. A preclinical experimental study

Berce

Muresan

Şoriţău

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Efficiency of Cryotherapy in Avoiding Myringotomy Closure in Rats

Cited by 3 publications

References 14 publications

In vivo assessment of bone marrow toxicity by gold nanoparticle-based bioconjugates in Crl:CD1(ICR) mice

In vivo assessment of bone marrow toxicity by gold nanoparticle-based bioconjugates in Crl:CD1(ICR) mice

The Impact of Spray Cryotherapy on Lesion-Induced Osteitis in a New Murine Experimental Model

Cutaneous wound healing using polymeric surgical dressings based on chitosan, sodium hyaluronate and resveratrol. A preclinical experimental study

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