Efficiency of essential oils as antifungal agents against Aspergillus fumigatus KY026061causing allergic bronchopulmonaryaspergillosis (ABPA)

El-Zaher, Eman H.F. Abd; Shouny, Wagih Abd El Fattah El; Shabana, Samya Abas; El-Salam, Olaa Abd

doi:10.21608/ejbo.2019.6671.1262

Cited by 2 publications

(2 citation statements)

References 28 publications

(28 reference statements)

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“…In the present study, the antimicrobial activity of the extracellular siderophore TafC was carried out using inhibition zone diameter and results showed that TafC had antibacterial activity, these results matched with Chowdappa et al (2020) who revealed that the antibacterial activity of siderophores produced by Endophytic fungi was determined by a disc diffusion assay where the results proved that the siderophore exhibited the highest zone of inhibition on plant pathogens. Essential oils have been used as antifungal agents against A. fumigatus (Abd El-Zaher et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Isolation, Identification, and Characterization of Extracellular Siderophore Triacetylfusarinine C (TafC) Produced by Aspergillus fumigatus

Azel,

Sherif,

Moussa

et al. 2023

Egypt. J. Bot.

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Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 75 H YDROXAMATE siderophores are low molecular weight, high-affinity ferric ironchelating compounds which are excreted by Aspergillus fumigatus for iron transport extracellularly or iron storage intracellularly. Aspergillus fumigatus was isolated from different samples, then siderophores were extracted from A. fumigatus by phenol, and diethyl ether, and purified by amberlite XAD-16 resin. The chemical structure of siderophores was described by H¹NMR, ESI-MS/MS, and LC-MS/MS analysis. Also, antimicrobial activity was observed.From the elution profile of siderophores, the major peak was at fraction 28. As a result of H¹NMR analysis, the singlets assigned to the -C=O-CH=C (CH3) C-substructure were detected.The siderophore TafC had antibacterial activity against both G +ve and G -ve bacteria. Under different concentrations of iron, it can be deduced that the concentration of 0.03 mmol of iron at 20 hrs. incubation period was an ideal condition for mycelial fresh weight which positively affected, so production of siderophores increased. From chemical analysis, it was found that the chemical formula C 39 H 58 FeN 6 O 15 was consistent with the known siderophore N, N', N"triacetylfusarinine C (TafC).

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Section: Discussionmentioning

confidence: 99%

Isolation, Identification, and Characterization of Extracellular Siderophore Triacetylfusarinine C (TafC) Produced by Aspergillus fumigatus

Azel,

Sherif,

Moussa

et al. 2023

Egypt. J. Bot.

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“…It is known that phenolic compounds have the ability to alter cell permeability and disrupt the membrane lipid (6). Furthermore, the regulation and function of cell wall synthesis enzymes may also be affected leading to alternation in microbial cell growth and morphogenesis (37,38).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Biochemical and Ultrastructural Mechanisms Underlying the Antimicrobial Activity of Mimusops spp. Extracts

2020

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Antibiotic resistance is the major growing threat facing the pharmacological treatment of bacterial infections. Therefore, bioprospecting the medicinal plants could provide potential sources for antimicrobial agents. Mimusops, the biggest and widely distributed plant genus of family Sapotaceae, is used in traditional medicines due to its promising pharmacological activities. This study was conducted to elucidate the antimicrobial effect of three unexplored Mimusops spp. (M. kummel, M. laurifolia and M. zeyheri). Furthermore, the mechanisms underlying such antibacterial activity were studied. The Mimusops leaf extracts revealed significant antibacterial activities against the five tested bacterial strains with a maximum inhibition zone diameter of 22.0 mm against B. subtilis compared with standard antibiotic ciprofloxacin. The minimal inhibitory and bactericidal concentration values against tested Gram-positive and Gram-negative bacterial strains ranged from 3.15-12.5 µg/ml. However, weak antifungal effect was recorded against Candida albicans with MIC value ˃25 µg/ml. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay showed that M. caffra was the best antioxidant (IC50=14.75±0.028 µg/ml), while M. laurifolia was the least one (IC50=34.22±0.014 µg/ml). The phenolics in plant leaves extracts were identified and quantified by high performance liquid chromatography (HPLC) which revealed the presence of seven phenolic acids and four flavonoids. The abundant phenolic compounds were rutin (5.216±0.067 mg/g dried wt.) and gallic acid (0.296±0.068 mg/g) followed by myricetin (0.317±0.091 mg/g) then kaempferol (0.113±0.049 mg/g) as flavonoids. The antibacterial mechanism of M. laurifolia extract, as a representative species, induces ultrastructural changes in the model bacterium Staphylococcus aureus with cell wall and plasma membrane lysis as revealed by transmission electron microscopy. Overall, Mimusops species (M. laurifolia, M. kummel and M. zeyheri) are promising natural alternative sources for antimicrobial agents.

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Efficiency of essential oils as antifungal agents against Aspergillus fumigatus KY026061causing allergic bronchopulmonaryaspergillosis (ABPA)

Cited by 2 publications

References 28 publications

Isolation, Identification, and Characterization of Extracellular Siderophore Triacetylfusarinine C (TafC) Produced by Aspergillus fumigatus

Isolation, Identification, and Characterization of Extracellular Siderophore Triacetylfusarinine C (TafC) Produced by Aspergillus fumigatus

Investigation of the Biochemical and Ultrastructural Mechanisms Underlying the Antimicrobial Activity of Mimusops spp. Extracts

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