Efficient 2,4-dichloronitrobenzene removal in the coupled BES-UASB reactor: Effect of external voltage mode

Chen, Hui; Gao, Xinyi; Wang, Caiqin; Shao, Junjie; Xu, Xiangyang; Zhu, Liang

doi:10.1016/j.biortech.2017.06.010

Cited by 29 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genera Prosthecobacter and Zoogloea were important biodegraders of aniline in the 13 C-labeled treatments at 96 h. Chen et al [27] found that there was a homogeneous microbial community structure in the up-flow anaerobic sludge blanket (UASB) reactor with bio-electrochemical properties and chloronitrobenzenes in the seeding sludge, including Prosthecobacter (5.3%) and Thauera (4.6%), and they believed that Prosthecobacter, as an aniline-degrading bacterium, was worthy of in-depth investigation. Zoogloea was found to adhere to the surface of the ceramic particles of a biotrickling filter used to treat aniline [28] and was shown to degrade phenols, nitrogenous heterocyclic compounds [17] and nitrobenzene [29].…”

Section: Aniline-degrading Bacteria Identified In Aerobic Microcosmmentioning

confidence: 97%

Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Li,

Ghani,

Sun

et al. 2024

Catalysts

View full text Add to dashboard Cite

Aniline, a vital component in various chemical industries, is known to be a hazardous persistent organic pollutant that can cause environmental pollution through its manufacturing, processing, and transportation. In this study, the microcosms were established using sediment with a history of aniline pollution as an inoculum to analyze the aniline biodegradation under aerobic conditions through stable isotope probing (SIP) and isopycnic density gradient centrifugation technology. During the degradation assay, aniline that was 13C-labeled in all six carbons was utilized to determine the phylogenetic identity of the aniline-degrading bacterial taxa that incorporate 13C into their DNA. The results revealed that aniline was completely degraded in the microcosm after 45 and 69 h respectively. The bacteria affiliated with Acinetobacter (up to 34.6 ± 6.0%), Zoogloea (up to 15.8 ± 2.2%), Comamonas (up to 2.6 ± 0.1%), and Hydrogenophaga (up to 5.1 ± 0.6%) genera, which are known to degrade aniline, were enriched in the heavy fractions (the DNA buoyant density was 1.74 mg L−1) of the 13C-aniline treatments. Moreover, some rarely reported aniline-degrading bacteria, such as Prosthecobacter (up to 16.0 ± 1.6%) and Curvibacter (up to 3.0 ± 1.6%), were found in the DNA-SIP experiment. Gene families affiliated with atd, tdn, and dan were speculated to be key genes for aniline degradation based on the abundance in functional genes and diversity in different treatments as estimated using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States version 2 (PICRUSt2) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). This study revealed the functional bacteria and possible degradation genes for aniline degradation in simulated polluted environments through SIP. These findings suggest that important degrading bacteria for the transformation of aniline and potential degradation pathways may be useful in the effective application of bioremediation technologies to remediate aniline-contaminated sites.

show abstract

Section: Aniline-degrading Bacteria Identified In Aerobic Microcosmmentioning

confidence: 97%

Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Li,

Ghani,

Sun

et al. 2024

Catalysts

View full text Add to dashboard Cite

show abstract

“…were found in chocolate wastewater-based MFC anodes [36]. Alcohol-powered MFCs were the second dominant community members [37]. The A2 sequence type was 99% similar to uncultured Pseudomonas sp., which have been proven to self-produce phenazine-based mediators in the anodes of MFCs to improve electricity generation [38].…”

Section: The Anode Microbial Communitymentioning

confidence: 98%

Analysis of Anodes of Microbial Fuel Cells When Carbon Brushes Are Preheated at Different Temperatures

et al. 2017

View full text Add to dashboard Cite

Abstract:The anode electrode is one of the most important components in all microbial electrochemical technologies (METs). Anode materials pretreatment and modification have been shown to be an effective method of improving anode performance. According to mass loss analysis during carbon fiber heating, five temperatures (300, 450, 500, 600, and 750 • C) were selected as the pre-heating temperatures of carbon fiber brush anodes. Microbial fuel cell (MFC) reactors built up with these pre-heated carbon brush anodes performed with different power densities and Coulombic efficiencies (CEs). Two kinds of measuring methods for power density were applied, and the numerical values of maximum power densities diverged greatly. Reactors with 450 • C anodes, using both methods, had the highest power densities, and the highest CEs were found using 500 • C anode reactors. The surface elements of heat-treated carbon fibers were analyzed using X-ray photoelectron spectra (XPS), and C, O, and N were the main constituents of the carbon fiber. There were four forms of N1s at the surface of the polyacrylonitrile (PAN)-based carbon fiber, and their concentrations were different at different temperature samples. The microbial community of the anode surface was analyzed, and microbial species on anodes from every sample were similar. The differences in anode performance may be caused by mass loss and by the surface elements. For carbon brush anodes used in MFCs or other BESs, 450-500 • C preheating was the most suitable temperature range in terms of the power densities and CEs.

show abstract

“…For example, Zhang et al (2021) obtained a consortium of various bacterial communities while treating municipal sewage with Geobacter being insignificant comparatively. On the other hand, a control UASB fed with synthetic wastewater (sucrose and dichloronitrobenzen acting as the organic source) showed a higher relative abundance of Planctomyces, Prosthecobcter and Phenylobacterium (H. Chen et al, 2017).…”

Section: The Microbiology Of Uasb Biomassmentioning

confidence: 99%

“…UASB systems that implemented electrochemical systems such as microbial fuel cells, or constant voltage/current application yielded promising performance results (H. Chen et al, 2017;Yang et al, 2019;Zhen et al, 2017). The main characteristic in these systems is the development of electroactive biofilms attached on the electrodes which contributed to the enhanced performance.…”

Section: Novel Technologies To Improve Uasb Start-up and Subsequent E...mentioning

confidence: 99%

Enhancement of The Start-up of an Up-flow Anaerobic Sludge Blanket Reactor Using Electrochemically Enriched Biofilms. (c2022)

Abdallah¹

View full text Add to dashboard Cite

With rising issue of wastewater discharge and resulting pollution, anaerobic wastewater treatment has emerged as a prominent technology to overcome these issues. A myriad of anaerobic treatment techniques has demonstrated appreciable results in treating high strength wastewaters, with high-rate digesters being the popular option. The up-flow anaerobic sludge blanket reactor (UASB) is a high-rate anaerobic digester capable of achieving high COD removal rates under high organic and hydraulic loadings. A critical feature of the start-up of an UASB reactor is the acclimation of the inoculated biomass through a process known as granulation. Granulation is characterized by the agglomeration of specific types of bacterial and methanogenic archaea known to possess electroactive properties which allow cell to cell attachment through a mechanism known as direct interspecies electron transfer (DIET). Recently, several species known to be DIET participants, labeled as electroactive microbes, have been selectively enriched in microbial electrolysis cells known as bio-electrochemical reactors (BER) through applying constant electrode potentials favoring their proliferation through catalytic metabolism of reactor substrates. In this framework, the following thesis aimed to enhance the granulation and performance of an UASB reactor fed with high strength wastewater through supplementation with electroactive species enriched using BERs. An anodic BER (ABER) was poised at +400 mV vs Ag/AgCl to enrich electroactive bacterial species at the working electrode whereas a cathodic BER (CBER) was poised at -700 mV vs Ag/AgCl to enrich electrotrophic methanogens. After 5 feeding phases, the biofilms were extracted from the working electrodes of both BERs, in addition to the counter electrodes. An UASB reactor, operated at a HRT of 48 hours and OLR of 1 g-COD/L.d, was operated in two phases: Phase 1 being the control run and Phase 2 supplemented with the BER enriched biomass. Electrochemical analyses were performed on the BERs which showed the development of electrode respiring microbial communities based on cyclic voltammograms and chronoamperometric scans. Better COD and VSS removal rates were achieved in UASB Phase 2 as compared to Phase 1. Phase 2 COD removal of 85% was achieved in 20 days, whereas the same COD removal was achieved in 62 days for Phase 1. Settleability tests performed on the developed sludge in the UASB bed showed a highly granular sludge (SVI = 8.5 mL/gTSS) in Phase 2 compared to flocculant sludge (SVI = 33 mL/gTSS) in Phase 1 after 20 days of operation. The findings of this study prove that electrochemically enriched biofilms can be utilized in anaerobic digesters through direct supplementation of biofilms, rather than hybrid system integration, and can exhibit improved reactor performance.

show abstract

Efficient 2,4-dichloronitrobenzene removal in the coupled BES-UASB reactor: Effect of external voltage mode

Cited by 29 publications

References 46 publications

Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Analysis of Anodes of Microbial Fuel Cells When Carbon Brushes Are Preheated at Different Temperatures

Enhancement of The Start-up of an Up-flow Anaerobic Sludge Blanket Reactor Using Electrochemically Enriched Biofilms. (c2022)

Contact Info

Product

Resources

About