Efficient alachlor degradation by the filamentous fungus  Paecilomyces marquandii  with simultaneous oxidative stress reduction

Słaba, Mirosława; Różalska, Sylwia; Bernat, Przemysław; Szewczyk, Rafał; Piątek, Milena; Długoński, Jerzy

doi:10.1016/j.biortech.2015.08.045

Cited by 31 publications

(13 citation statements)

References 34 publications

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“…The washed fresh mycelium was homogenized (1:10 w/v) in an ice-cold mortar together with 50 mM sodium phosphate buffer (pH 7) containing 1% polyvinylpyrrolidone, 10 mM sodium ascorbate, and 1 mM EDTA. After centrifugation (20 min, 20,000× g ), the supernatant was used for the determination of antioxidant enzymatic activity [ 17 ]. The CAT activity was measured spectrophotometrically at 240 nm by a method proposed by Dhindsa et al [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader

et al. 2018

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The study reports the response to herbicide of the 2,4-dichlorophenoxyacetic acid (2,4-D)–degrading fungal strain Umbelopsis isabellina. A comparative analysis covered 41 free amino acids as well as 140 lipid species of fatty acids, phospholipids, acylglycerols, sphingolipids, and sterols. 2,4-D presence led to a decrease in fungal catalase activity, associated with a higher amount of thiobarbituric acid-reactive substances (TBARS). Damage to cells treated with the herbicide resulted in increased membrane permeability and decreased membrane fluidity. Detailed lipidomic profiling showed changes in the fatty acids composition such as an increase in the level of linoleic acid (C18:2). Moreover, an increase in the phosphatidylethanolamine/phosphatidylcholine ratio was observed. Analysis of fungal lipid profiles revealed that the presence of 2,4-D was accompanied by the accumulation of triacylglycerols, a decrease in ergosterol content, and a considerable rise in the level of sphingolipid ceramides. In the exponential phase of growth, increased levels of leucine, glycine, serine, asparagine, and hydroxyproline were found. The results obtained in our study confirmed that in the cultures of U. isabellina oxidative stress was caused by 2,4-D. The herbicide itself forced changes not only to membrane lipids but also to neutral lipids and amino acids, as the difference of tested compounds profiles between 2,4-D—containing and control samples was consequently lower as the pesticide degradation progressed. The presented findings may have a significant impact on the basic understanding of 2,4-D biodegradation and may be applied for process optimization on metabolomic and lipidomic levels.

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Section: Methodsmentioning

confidence: 99%

2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader

et al. 2018

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“…Moreover, for Paecilomyces marquandii (Massee) S. Hughes, the pH of the medium affects the production of reactive oxygen species during biodegradation of the pesticide alachlor. Neutral pH was favourable both for alachlor biodegradation and for oxidative stress reduction (Słaba et al 2015). The effectiveness of mycelial protection by anti-oxidative enzymes from oxidative stress was observed to be dependent on the environmental pH, exposure time and fungal growth phase (Słaba et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Neutral pH was favourable both for alachlor biodegradation and for oxidative stress reduction (Słaba et al 2015). The effectiveness of mycelial protection by anti-oxidative enzymes from oxidative stress was observed to be dependent on the environmental pH, exposure time and fungal growth phase (Słaba et al 2015). Interestingly, the synthesis of laccase and other extracellular peroxidases by some filamentous fungi was higher in the presence of intracellular oxidative stress (Chanda et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Metabolic synergies in the biotransformation of organic and metallic toxic compounds by a saprotrophic soil fungus

Ceci

Pinzari

Riccardi

et al. 2017

Appl Microbiol Biotechnol

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The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil containing high concentrations of HCH isomers and potentially toxic elements (Pb, V). Experiments were performed in order to analyse the tolerance/resistance of this fungus to xenobiotics and to shed further light on fungal potential in inorganic and organic biotransformations. The aim was to examine the ecological and bioremedial potential of this fungus verifying the presence of mechanisms that allow it to transform HCH isomers and metals under different extreme test conditions. To our knowledge, this work is the first to provide evidence on the biotransformation of HCH mixtures, in combination with toxic metals, by a saprotrophic non-white-rot fungus and on the metabolic synergies involved.

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“…Alachlor (2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide) is one of the most frequently used herbicides in weed control, early inhibiting their development. This compound belongs to the chloroacetamide class and it is widely used to protect corn, rice, soybean, peanut, and cotton crops 1 . Alachlor can be degraded by microorganisms present in soils.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical degradation of aqueous alachlor and atrazine: products identification, lipophilicity, and ecotoxicity

2019

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This work studied the electrochemical degradation of alachlor and atrazine (alone and mixed with each other) using a filter-press cell, a dimensionally stable anode (DSA Ti/Ru0.3Ti0.7O2), initial pH 3.0, and temperature at 25 °C. The best operational conditions for alachlor (0.33 mmol L-1) degradation were obtained by a 32 factorial design, in which the factors/levels were: NaCl concentration (0.05, 0.1, and 0.15 mol L-1) and current density (10, 30, and 50 mA cm-2). Thus, 93.1% alachlor removal and 71.2% mineralization were achieved using 0.15 mol L-1 NaCl and 30 mA cm-2. In addition, the initial degradation products (DPs) of alachlor and atrazine were identified by liquid chromatography coupled to mass spectrometry (LC-MS). Acute and chronic ecotoxicities for three trophic levels (fishes, daphnids and green algae) and lipophilicity (log D, pH 7.4) of the DPs were also estimated using the ECOSAR 1.11 and ChemAxon Calculator software, respectively. The present study showed that the electrochemical degradation is an efficient method for removing the herbicides alachlor and atrazine from water and that the DPs formed have lower pollution potential than their original compounds.

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Efficient alachlor degradation by the filamentous fungus Paecilomyces marquandii with simultaneous oxidative stress reduction

Cited by 31 publications

References 34 publications

2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader

2,4-dichlorophenoxyacetic acid-induced oxidative stress: Metabolome and membrane modifications in Umbelopsis isabellina, a herbicide degrader

Metabolic synergies in the biotransformation of organic and metallic toxic compounds by a saprotrophic soil fungus

Electrochemical degradation of aqueous alachlor and atrazine: products identification, lipophilicity, and ecotoxicity

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