Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

Tonelli, Fernanda Maria Policarpo; Lacerda, Samyra Maria dos Santos Nassif; Paiva, N. C. O.; Lemos, Moline Severino; Jesus, Augusto C. de; Pacheco, Flávia G.; Corrêa, José Dias; Ladeira, Luiz Orlando; Furtado, Clascídia Aparecida; França, Luiz R.; Resende, Rodrigo R.

doi:10.1039/c6ra07010h

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…They were previously successfully tested as transfection agents in difficult-to-transfect-cells, including neonatal rat cardiomyocytes, rat dorsal root ganglion cells (DRG cells), human neuroblastoma cell line (U37311 cells), rat glial tumour cell line (C612 cells), 31 and fish spermatogonial stem cells. 32 Moreover, they presented promising results for antimony (Sb) delivery on leishmania infected murine macrophage, thereby targeting and reducing chemotherapy in in-vitro assays. 33…”

Section: Introductionmentioning

confidence: 99%

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44⁺ cells

Tótaro

2021

Preprint

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The development degree of mammals at birth is markedly variable especially between placental and non-placental species. In marsupial mammals the organogenesis is completed during a period called external gestation, when the young animal remains at the maternal pouch being breastfed for, on average 90 days. This study reported the relevant morphological features found in the pancreas of the Brazilian opossum Didelphis aurita, a marsupial mammal, during its intramarsupial development. Newborns and young opossums removed from the pouch were divided into groups according to their average body length. The animals with few hours of intramarsupial life had visibly immature pancreas, due to the presence of early arrangement of endocrine and exocrine components. There was an only very large pancreatic duct, and blood vessels full of nucleated erythrocytes. In the individuals who were in the second third of intramarsupial development, the exocrine components were arranged in developing acini and endocrine cells were found arranged in early islets. The presence of connective tissue and the division of the organ into lobes became more evident at this stage. In the last third of the external gestation, we found better structured acini with relatively small lumens. The islets were well-organized, and the presence of connective tissue around them was the most evident. Morphometric analyses showed considerable variations in the proportion of pancreatic structural components between the stages of intramarsupial development, indicating that marsupial pancreas undergoes morphological modifications and grows during the period of external gestation.

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Section: Introductionmentioning

confidence: 99%

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44⁺ cells

Tótaro

2021

Preprint

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“…Nile tilapia SSCs were obtained through primary culture from adult fish testis 12 22 23 24 , and 10 6 cells per dish were exposed to 1 mL of lentiviral solution (titre = 6.5 × 10 5 TU/mL). The cells were incubated at 33 °C and 5% CO 2 for 24 h, and the aliquot was submitted to flow cytometry in order to assess the percentage of fluorescent cells before selection.…”

Section: Methodsmentioning

confidence: 99%

Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis

Tonelli

Lacerda

Procópio

et al. 2017

Sci Rep

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Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: spermatogonial stem cell (SSC) genetic modification and transplantation (SC), in vivo transduction of gametes (GT), and fertilised egg transduction (ET). The SC protocol using larvae generates animals with sustained production of modified sperm (80% of animals with 77% maximum sperm fluorescence [MSF]), but is a time-consuming protocol (sexual maturity in Nile tilapia is achieved at 6 months of age). GT is a faster technique, but the modified gamete production is temporary (70% of animals with 52% MSF). ET is an easier way to obtain mosaic transgenic animals compared to microinjection of eggs, but non-site-directed integration in the fish genome can be a problem. In this study, PI3Kc2α gene disruption impaired development during the embryo stage and caused premature death. The manipulator should choose a technique based on the time available for transgenic obtainment and if this generation is required to be continuous or not.

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“…84 In contrast to other available techniques like electroporation and commercial reagents, the use of nanomaterials induces negligible cell death thereby increasing the potential of nanomaterials for transgenic technology in fish. 84 All the studies suggest the potential of NPs in sperm-mediated transfer technology in aquaculture species, especially in the production of transgenic fish. The pictorial representation of sperm-mediated transfer is shown in Figure 2.…”

Section: Sperm-mediated Transfermentioning

confidence: 99%

“…In Nile tilapia ( O. niloticus ), nanomaterials like gold nanorods (45 nm), multiwalled carbon nanotubes (MWCNTs, 40 nm), nanographene oxide (thin films) and nanodiamond (5 nm) were complexed to plasmid DNA (pAmCyan1‐N1) and delivered to spermatogonial stem cells 84 . In contrast to other available techniques like electroporation and commercial reagents, the use of nanomaterials induces negligible cell death thereby increasing the potential of nanomaterials for transgenic technology in fish 84 . All the studies suggest the potential of NPs in sperm‐mediated transfer technology in aquaculture species, especially in the production of transgenic fish.…”

Section: Nanotechnology Applications In Semen Sperm‐related Biotechno...mentioning

confidence: 99%

Nanotechnology in reproduction, breeding and conservation of fish biodiversity: Current status and future potential

Bhat

2022

Reviews in Aquaculture

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The reproductive capability of an organism is the main factor to determine the stability of the population. Till now, numerous reproductive strategies have been used, to successfully breed the fish in captivity but the desired performance is yet to be achieved. Nanotechnology has transformed the reproductive capacity of higher animals to a greater extent and has a huge potential in the aquaculture sector too. The use of nanotechnology in fish reproduction and breeding is limited to the delivery of drugs, hormones, vaccines, or genes and still, an immense scope is left in the area that needs to be explored. Here, the recent progress of nanotechnology in fish reproduction, breeding and conservation of germplasm is reviewed. Further, the key successes in the development of cryopreservation, semen imaging, sperm sorting, germplasm preservation, prototypes of smart nanobiosensors and drug delivery systems in animals including fish are highlighted. The potential future opportunities for nanotechnology to enhance reproductive output and conservative management of fish have been presented. In general, the current review summarises some of the applications of nanotechnology that are performing well in higher animals and could be a game-changer in manipulating the reproductive potential or conserving the fish germplasm.

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Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

Abstract: The nanomaterials MWCNTs, NGO and NRs can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested.

Cited by 5 publications

References 25 publications

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44⁺ cells

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44⁺ cells

Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis

Nanotechnology in reproduction, breeding and conservation of fish biodiversity: Current status and future potential

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Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

Abstract: The nanomaterials MWCNTs, NGO and NRs can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested.

Cited by 5 publications

References 25 publications

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44+ cells

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44+ cells

Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis

Nanotechnology in reproduction, breeding and conservation of fish biodiversity: Current status and future potential

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Product

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Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44⁺ cells

Multifunctional phosphate based nanoparticles as a platform for imaging, targeting and doxorubicin delivery to human breast cancer CD44⁺ cells