Efficient and simple plant regeneration via organogenesis from leaf segment cultures of persimmon (Diospyros kaki thunb.)

Abstract

An efficient and simple plant regeneration system via organogenesis from leaf segments of persimmon (Diospyros kaki Thunb.) cultivars`Fuyu' and`Nishimurawase' has been developed. The regeneration capacity was influenced by the culture vessels, gelling agents, plant growth regulators, and light conditions. Leaf explants taken from in vitro shoots were cultured on a modified Murashige and Skoog medium (MS 1 2 N) for 16 wk without transfer to fresh medium. Adventitious shoots appeared after 4 and 8 wk in culture of`Nishimurawase' and`Fuyu' tissues, respectively. The culture of leaf explants in Erlenmeyer flasks with medium containing 4 g l 21 agar enhanced shoot formation in comparison to media with increased agar concentrations. Optimal shoot regeneration was obtained with 5 mg l 21 (22.8 mM) zeatin and 0.1 mg l 21 (0.05 mM) indole-3-butyric acid (IBA) for`Nishimurawase', and 10 mg l 21 (45.6 mM) zeatin and 0.1 mg l 21 (0.05 mM) IBA for`Fuyu'. Shoot regeneration frequencies in both cultivars were 100%, and shoot numbers per explant reached up to 9.2 for`Nishimurawase' and 2.2 for`Fuyu'. Dark incubation during the first 4±5 wk was the most effective condition to successfully influence shoot regeneration in both cultivars. While dark incubation was essential for adventitious shoot formation by`Fuyu', it was only slightly beneficial to`Nishimurawase'. More than 80% of the regenerated shoots rooted within 4 wk on hormone-free MS 1 2 N medium after having been dipped for 30 s in 250 mg l 21 (1.1 mM) IBA solution.

Factors Affecting Plant Regeneration from Leaf Tissues of Buddleia Species

“…Limited and controversial information is available regarding the effect of dark treatment on plant regeneration. Although there are reports that dark treatment significantly increased plant regeneration of apple, purslane, persimmon, castors, and so on (Ahn et al, 2007;Choi et al, 2001;Fasolo et al, 1989;Fiore et al, 1997;Gu and Zhang, 2005;Rossi-Hassani and Zryd, 1995), other researches showed no stimulatory effect of dark treatment on plant regeneration of Rubus and Juncus accuminatus (Fiola et al, 1990;Sarma and Rogers, 1998). Our other research results on several species, including aspen, birch, chokecherry, periwinkle, and viburnum, showed that the effect of dark was not only species-but also genotype-dependent (unpublished data).…”

Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant

“…Shoots that developed roots using this method were transplanted to small pots; of the 80 rooted shoots transferred, 60 (75%) plantlets survived in the greenhouse. The induction of roots by the basal dipping of shoots developed in vitro in an IBA solution for short periods and subsequent transfer to growth regulator-free medium has been reported in Diospyros kaki (Choi et al 2001) and Ceratonia siliqua (Romano et al 2002). In the present study, the rooted shoots were transferred directly to the greenhouse following the dipping in auxin solution.…”

Adventitious bud formation through nodule induction by thidiazuron in cultured leaf segments of the Japanese persimmon (Diospyros kaki Thunb.)

“…It regenerated as many as 66 shoots per leaf segment (5 mmϫ5 mm), which is more efficient than reported previously (Choi et al 2001). Although it should be examined how frequently somaclonal variation occurs through culture with TDZ, this protocol may be applicable in agricultural practice.…”