Efficient Assay for Total Antioxidant Capacity in Human Plasma Using a 96-Well Microplate

Kambayashi, Yasuhiro; Bình, Nguyễn Thanh; Asakura, Hiroki; Hibino, Yuri; Hitomi, Yoshiaki; Nakamura, Hiroyuki; Oginö, Kazuhíde

doi:10.3164/jcbn.08-162

Cited by 119 publications

(63 citation statements)

References 34 publications

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“…6. The contribution of ABTS assay-microplate in evaluating TAC of our results was in agreement with (Kambayashi et al 2009). In summary, the results of antioxidant scavenging activity by ABTS method showed that CP has the same as the effect of Trolox, against the oxidation of ABTS compared with the negative control as given in Fig.…”

Section: Discussionsupporting

confidence: 91%

“…Furthermore, the accuracy of these assays remains relatively ambiguous. An efficient assay for the assessment of TAC was currently developed and validated using a 96-well microplate (Kambayashi et al 2009;Erel 2004;Lussignoli et al 1999). Overall, Cocoa and its derived products (cocoa powder, cocoa liquor and chocolates) contain varied polyphenol contents and possess different levels of antioxidant potentials.…”

Section: Introductionmentioning

confidence: 99%

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Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

et al. 2013

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The antioxidant components of cocoa powder, which is rich in polyphenols, were isolated using column chromatography and high performance liquid chromatography. Polyphenolic compounds were then characterized by high-performance liquid chromatography/Ultraviolet and electronspray ionization-tandem mass spectrometry (HPLC-UV-/ESI-MS-MS). As a result, five phenolic compounds were detected. In this study we also investigated scavenging or the total antioxidant capacity (%) of cocoa polyphenol (CP) fractionated from cocoa powder extract. 114.0 mg/g of gallic acid -equivalent phenolics and 94.3 mg/ g catechin-equivalent flavonoids were quantified in this extract. Their free radical-scavenging activity was assessed by 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) assay, β-carotene bleaching test, and xanthine oxidase inhibitory activity (OX). Total antioxidant capacity (TAC) was further assessed against the myoglobin-induced oxidation of 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid (ABTS) and expressed as Trolox equivalent. A high correlation between TAC and phenolic contents indicated that phenolic compounds from cocoa were a major contributor of antioxidant activity (0.967≤r≤1.00). CP extract had significantly (P <0.05) potential antioxidant activities with various concentrations. These results suggest that Polyphenols-rich cocoa extract possess prominent medical properties and can be exploited as natural drug to treat free radical associated diseases.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

et al. 2013

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“…However, WAX treatment significantly ameliorated the depletion of all measured factors compared with the control group (Table 1). TAC is a reliable biomarker of total antioxidant level, and GSH, SOD and catalase are three essential antioxidants or free radical quenchers (Alscher et al, 2002;Beers & Sizer, 1952;Evans & Ellman, 1959;Kambayashi et al, 2009). These data indicated that the hepatoprotective action of WAX is mediated through its effects on the antioxidant system in DMN-induced subchronic liver injury.…”

Section: Resultsmentioning

confidence: 78%

“…The TAC in liver tissue was examined by the method described previously (Kambayashi et al, 2009). The results were expressed as mM equivalent of gallic acid.…”

Section: Determination Of Total Antioxidant Capacity (Tac) Total Glumentioning

confidence: 99%

Hepatoprotective effect ofAmomum xanthoidesagainst dimethylnitrosamine-induced sub-chronic liver injury in a rat model

Wang

Choi

et al. 2013

Pharmaceutical Biology

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Son (2013) Hepatoprotective effect of Amomumxanthoides against dimethylnitrosamine-induced sub-chronic liver injury in a rat model, Pharmaceutical Biology, 51:7, 930-935, DOI: 10.3109/13880209.2013 and 100 mg/kg) was given once a day for 3 weeks. Results and conclusion: WAX (100 mg/kg) significantly attenuated the DMN-induced excessive release of alanine aminotransferase (123.6 IU/L), aspartate aminotransferase (227.9 IU/L), alkaline phosphatase (820.9 IU/L) and total bilirubin (0.50 g/dL) in serum (p50

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“…Trolox equivalent performed with the ABTS solution represents an important screening tool for oxidative stressrelated diseases by monitoring plasma TAC (Kambayashi et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

Coneac

Orăsan

Leucuţa

et al. 2017

Not Bot Horti Agrobo

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Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg -1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg -1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

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Efficient Assay for Total Antioxidant Capacity in Human Plasma Using a 96-Well Microplate

Cited by 119 publications

References 34 publications

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties

Hepatoprotective effect ofAmomum xanthoidesagainst dimethylnitrosamine-induced sub-chronic liver injury in a rat model

Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

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