Efficient chondrogenic differentiation of mesenchymal cells in micromass culture by retroviral gene transfer of BMP-2

Carlberg, Alyssa L.; Pucci, Bruna; Rallapalli, Ravikumar; Tuan, Rocky S.; Hall, D.

doi:10.1046/j.1432-0436.2001.670405.x

Cited by 96 publications

(82 citation statements)

References 46 publications

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“…Conversely, delivery of the BMP-2 cDNA into C3H10T1/2 cells subsequently incorporated into micromass culture was found to induce chondrogenic differentiation throughout the aggregate. 86,87 Similar results were obtained following adenoviral-mediated delivery of TGF-b1 or BMP-2 into human mesenchymal progenitor cells isolated from bone marrow. Human MSCs transduced to express human BMP-2 were found to form ectopic bone and cartilage following injection into mice.…”

Section: Gene Transfer To Chondroprogenitor Cellssupporting

confidence: 57%

Gene-based approaches for the repair of articular cartilage

2004

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Section: Gene Transfer To Chondroprogenitor Cellssupporting

confidence: 57%

Gene-based approaches for the repair of articular cartilage

2004

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“…During chondrogenesis in explant culture systems, BMP signaling induces the cell cycle inhibitor p21 (Carlberg et al, 2001), indicating that BMPs couple cell cycle withdrawal with cartilage differentiation. However, the BMP pathway target Sox9 can also bind and activate the p21 promoter (Panda et al, 2001).…”

Section: Growth and Maintenance Of Digits And Jointsmentioning

confidence: 99%

Mechanisms of digit formation: Human malformation syndromes tell the story

Stricker

Mundlos

2011

Developmental Dynamics

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Identifying the genetic basis of human limb malformation disorders has been instrumental in improving our understanding of limb development. Abnormalities of the hands and/or feet include defects affecting patterning, establishment, elongation, and segmentation of cartilaginous condensations, as well as growth of the individual skeletal elements. While the phenotype of such malformations is highly diverse, the mutations identified to date cluster in genes implicated in a limited number of molecular pathways, namely hedgehog, Wnt, and bone morphogenetic protein. The latter pathway appears to function as a key molecular network regulating different phases of digit and joint development. Studies in animal models not only extended our insight into the pathogenesis of these conditions, but have also contributed to our understanding of the in vivo functions and interactions of these key players. This review is aimed at integrating the current understanding of human digit malformations into the increasing knowledge of the molecular mechanisms of digit development. Developmental Dynamics 240:990-1004,

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“…The family of BMPs has a pivotal role in prechondrogenic condensations and the transition of chondroprogenitor cells into chondrocytes [135][136][137]. Specifically, BMP-2 is expressed in the condensing mesenchyme of the developing limb [138], and regulates chondrogenic development of mesenchymal progenitors [139][140][141]. BMPs were demonstrated to induce chondrogenesis of MSCs in vitro [142,143].…”

Section: Vivomentioning

confidence: 99%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

210

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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Efficient chondrogenic differentiation of mesenchymal cells in micromass culture by retroviral gene transfer of BMP-2

Cited by 96 publications

References 46 publications

Gene-based approaches for the repair of articular cartilage

Gene-based approaches for the repair of articular cartilage

Mechanisms of digit formation: Human malformation syndromes tell the story

Cartilage tissue engineering for degenerative joint disease☆

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