Efficient Delivery of Antisense Oligodeoxyribonucleotide G3139 by Human Serum Albumin-Coated Liposomes

Weecharangsan, Wanlop; Yu, Bo; Zheng, Yu; Liu, Shujun; Pang, Jiu Xia; Lee, L. James; Marcucci, Guido; Lee, Robert J.

doi:10.1021/mp900150g

Cited by 26 publications

(19 citation statements)

References 24 publications

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“…The materialisation of the idea of interfering with expressions/activities of specific Bcl-2 targets in cancerous cells challenges us to develop a complete understanding of the behaviour of Bcl-2 proteins in response to different anticancer agents in various neoplasms. Although use of antisense oligonucleotides has been challenged with efficient drug delivery issues in clinical trials, significant achievements of non-peptidic small molecule inhibitors (SMI) of antiapoptotic Bcl-2 proteins are ensuring the acceleration of efforts to design Bcl-2 manipulation-based therapeutic approaches [37]–[44].…”

Section: Discussionmentioning

confidence: 99%

Pramanicin Analog Induces Apoptosis in Human Colon Cancer Cells: Critical Roles for Bcl-2, Bim, and p38 MAPK Signaling

et al. 2013

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Pramanicin (PMC) is an antifungal agent that was previously demonstrated to exhibit antiangiogenic and anticancer properties in a few in vitro studies. We initially screened a number of PMC analogs for their cytotoxic effects on HCT116 human colon cancer cells. PMC-A, the analog with the most potent antiproliferative effect was chosen to further interrogate the underlying mechanism of action. PMC-A led to apoptosis through activation of caspase-9 and -3. The apoptotic nature of cell death was confirmed by abrogation of cell death with pretreatment with specific caspase inhibitors. Stress-related MAPKs JNK and p38 were both activated concomittantly with the intrinsic apoptotic pathway. Moreover, pharmacological inhibition of p38 proved to attenuate the cell death induction while pretreatment with JNK inhibitor did not exhibit a protective effect. Resistance of Bax −/− cells and the protective nature of caspase-9 inhibition indicate that mitochondria play a central role in PMC-A induced apoptosis. Early post-exposure elevation of cellular Bim and Bax was followed by a marginal Bcl-2 depletion and Bid cleavage. Further analysis revealed that Bcl-2 downregulation occurs at the mRNA level and is critical to mediate PMC-A induced apoptosis, as ectopic Bcl-2 expression substantially spared the cells from death. Conversely, forced expression of Bim proved to significantly increase cell death. In addition, analyses of p53−/− cells demonstrated that Bcl-2/Bim/Bax modulation and MAPK activations take place independently of p53 expression. Taken together, p53-independent transcriptional Bcl-2 downregulation and p38 signaling appear to be the key modulatory events in PMC-A induced apoptosis.

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Section: Discussionmentioning

confidence: 99%

Pramanicin Analog Induces Apoptosis in Human Colon Cancer Cells: Critical Roles for Bcl-2, Bim, and p38 MAPK Signaling

et al. 2013

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“…As a result, the liposome collapses and fuses with the endosomal membrane which then leads to release of liposome contents into the cytoplasm 2 . Cellular uptake of HSA (human serum albumin)-coated liposome-ODN complexes used for Bcl-2 mRNA downregulation in KB (HeLa derivative) human oral carcinoma cells was found to be about three-fold higher compared to uncoated liposomes, and six-fold higher than naked As-ODNs 24 . Targeted action towards specific cells can be achieved by conjugating folate or transferrin to the liposome to assist receptor-mediated endocytosis.…”

Section: Liposomesmentioning

confidence: 98%

Antisense Oligonucleotides as Therapeutics and their Delivery

Gurav¹,

Ganga²

2017

Current Science

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Antisense oligonucleotides are novel, highly selective inhibitors or modulators of gene expression. Attention is now being paid for the use of these agents not only in the treatment of genetic disorders but also for nongenetic disorders, where the treatment involves modulation of gene expression. However, antisense oligonucleotides face immense challenges for their use as therapeutics and successful application of these agents requires appropriate design and delivery strategies to increase their stability and intracellular uptake.

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“…In another approach, BSA-coated liposomes were thermally denatured (DBL) and encapsulated with the drug doxorubicin. The DBLs showed higher intracellular uptake and stability in plasma in comparison to Doxil Õ , Titusville, NJ (Weecharangsan et al, 2009). …”

Section: Lipid Npsmentioning

confidence: 99%

Albumin corona on nanoparticles – a strategic approach in drug delivery

2015

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Nanomaterials have been used widely for delivery of therapeutic agents. Protein-nanoparticle (NP) complexes have gained importance as vehicles for targeted drug delivery due to increased ease of administration, stability and half-life of drug, and reduced toxic side effects. Designing of phospholipid-bovine serum albumin (BSA) complexes and stealth NPs with BSA has paved the way for drug delivery carriers with prolonged blood circulation times. Preformed albumin corona has shown to decrease non-specific association and thereby reduce the clearance rate. Albumin corona has enabled the localization of drug carriers in specific tissues such as liver and heart, thus regulating biodistribution. Tailored albumin-NP conjugates have also enabled controlled degradation of NP and drug release. However, the binding of albumin with NP is associated with conformational and functional modulations in protein as observed with silver, gold and superparamagnetic iron oxide NPs. In this review, we highlight the various potential albumin-NP hybrids as nano drug carriers.

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Efficient Delivery of Antisense Oligodeoxyribonucleotide G3139 by Human Serum Albumin-Coated Liposomes

Cited by 26 publications

References 24 publications

Pramanicin Analog Induces Apoptosis in Human Colon Cancer Cells: Critical Roles for Bcl-2, Bim, and p38 MAPK Signaling

Pramanicin Analog Induces Apoptosis in Human Colon Cancer Cells: Critical Roles for Bcl-2, Bim, and p38 MAPK Signaling

Antisense Oligonucleotides as Therapeutics and their Delivery

Albumin corona on nanoparticles – a strategic approach in drug delivery

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