Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation

Das, Joydeep; Choi, Yun‐Jung; Yasuda, Hideyo; Han, Jung Soo; Park, Chankyu; Song, Hyuk; Bae, Hojae; Kim, Jin‐Hoi

doi:10.1038/srep33784

Cited by 35 publications

(19 citation statements)

References 90 publications

(107 reference statements)

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“…Chemical non-viral methods employ liposomes, dendrimers, inorganic nanoparticles, magnetic nanoparticles (MNPs) or polymers as transfection agents. High transfection efficiency was reported using poly (ethylenimine) (PEI) in combination with gold or silica nanoparticles[ 54 , 55 ].…”

Section: Methods To Enhance Stem Cell Homingmentioning

confidence: 99%

Stem cells as delivery vehicles for regenerative medicine-challenges and perspectives

Lăbuşcă

Herea

Mashayekhi³

2018

WJSC

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The use of stem cells as carriers for therapeutic agents is an appealing modality for targeting tissues or organs of interest. Combined delivery of cells together with various information molecules as therapeutic agents has the potential to enhance, modulate or even initiate local or systemic repair processes, increasing stem cell efficiency for regenerative medicine applications. Stem-cell-mediated delivery of genes, proteins or small molecules takes advantage of the innate capability of stem cells to migrate and home to injury sites. As the native migratory properties are affected by in vitro expansion, the existent methods for enhancing stem cell targeting capabilities (modified culture methods, genetic modification, cell surface engineering) are described. The role of various nanoparticles in equipping stem cells with therapeutic small molecules is revised together with their class-specific advantages and shortcomings. Modalities to circumvent common challenges when designing a stem-cell-mediated targeted delivery system are described as well as future prospects in using this approach for regenerative medicine applications.

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Section: Methods To Enhance Stem Cell Homingmentioning

confidence: 99%

Stem cells as delivery vehicles for regenerative medicine-challenges and perspectives

Lăbuşcă

Herea

Mashayekhi³

2018

WJSC

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“…Inorganic-gold nanoparticles (Olton et al, 2007;Arvizo et al, 2010;Ding et al, 2014;Wegman et al, 2014;Das et al, 2016;Yang et al, 2018) High biocompatibility. Tunable features (e.g., size and surface coatings).…”

Section: Advantages Limitations Applicationsmentioning

confidence: 99%

Non-viral Gene Delivery Methods for Bone and Joints

Gantenbein

Tang

Guerrero

et al. 2020

Front. Bioeng. Biotechnol.

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Viral carrier transport efficiency of gene delivery is high, depending on the type of vector. However, viral delivery poses significant safety concerns such as inefficient/unpredictable reprogramming outcomes, genomic integration, as well as unwarranted immune responses and toxicity. Thus, non-viral gene delivery methods are more feasible for translation as these allow safer delivery of genes and can modulate gene expression transiently both in vivo, ex vivo, and in vitro. Based on current studies, the efficiency of these technologies appears to be more limited, but they are appealing for clinical translation. This review presents a summary of recent advancements in orthopedics, where primarily bone and joints from the musculoskeletal apparatus were targeted. In connective tissues, which are known to have a poor healing capacity, and have a relatively low cell-density, i.e., articular cartilage, bone, and the intervertebral disk (IVD) several approaches have recently been undertaken. We provide a brief overview of the existing technologies, using nano-spheres/engineered vesicles, lipofection, and in vivo electroporation. Here, delivery for microRNA (miRNA), and silencing RNA (siRNA) and DNA plasmids will be discussed. Recent studies will be summarized that aimed to improve regeneration of these tissues, involving the delivery of bone morphogenic proteins (BMPs), such as BMP2 for improvement of bone healing. For articular cartilage/osteochondral junction, non-viral methods concentrate on targeted delivery to chondrocytes or MSCs for tissue engineering-based approaches. For the IVD, growth factors such as GDF5 or GDF6 or developmental transcription factors such as Brachyury or FOXF1 seem to be of high clinical interest. However, the most efficient method of gene transfer is still elusive, as several preclinical studies have reported many different non-viral methods and clinical translation of these techniques still needs to be validated. Here we discuss the non-viral methods applied for bone and joint and propose methods that can be promising in clinical use.

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“…However, the application of nonviral systems in gene therapy has been limited due to their low transfection efficiency and transient expression of the transgene [ 92 ]. Liposomes and polymers are among traditional nonviral delivery systems shown that transiently transfect MSCs [ 90 , 93 – 95 ]. Minicircles are small DNA-based vectors lacking prokaryotic backbone sequences that are typically present in conventional plasmid vectors.…”

Section: Mesenchymal Stromal/stem Cells (Mscs)mentioning

confidence: 99%

Genetically Modified Mesenchymal Stromal/Stem Cells: Application in Critical Illness

Varkouhi

Monteiro

Tsoporis

et al. 2020

Stem Cell Rev and Rep

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Critical illnesses including sepsis, acute respiratory distress syndromes, ischemic cardiovascular disorders and acute organ injuries are associated with high mortality, morbidity as well as significant health care system expenses. While these diverse conditions require different specific therapeutic approaches, mesenchymal stem/stromal cell (MSCs) are multipotent cells capable of self-renewal, tri-lineage differentiation with a broad range regenerative and immunomodulatory activities, making them attractive for the treatment of critical illness. The therapeutic effects of MSCs have been extensively investigated in several pre-clinical models of critical illness as well as in phase I and II clinical cell therapy trials with mixed results. Whilst these studies have demonstrated the therapeutic potential for MSC therapy in critical illness, optimization for clinical use is an ongoing challenge. MSCs can be readily genetically modified by application of different techniques and tools leading to overexpress or inhibit genes related to their immunomodulatory or regenerative functions. Here we will review recent approaches designed to enhance the therapeutic potential of MSCs with an emphasis on the technology used to generate genetically modified cells, target genes, target diseases and the implication of genetically modified MSCs in cell therapy for critical illness.

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Efficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation

Cited by 35 publications

References 90 publications

Stem cells as delivery vehicles for regenerative medicine-challenges and perspectives

Stem cells as delivery vehicles for regenerative medicine-challenges and perspectives

Non-viral Gene Delivery Methods for Bone and Joints

Genetically Modified Mesenchymal Stromal/Stem Cells: Application in Critical Illness

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