Efficient DNA-Mediated Electron Transport in Ionic Liquids

Xuan, Shuguang; Meng, Zhenyu; Wu, Xiangyang; Wong, Jiun-Ru; Devi, Gitali; Yeow, Edwin K. L.; Shao, Fangwei

doi:10.1021/acssuschemeng.6b01605

Cited by 22 publications

(25 citation statements)

References 77 publications

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“…The similar γ values for both double helical structures suggested that the HT mechanisms in A- and B-form duplexes may share common features while mediating hole delocalization over adenine tracts, which was not unexpected due to the fact that only subtle variation on the dynamic motion and stacking structure of the base pairs occurred between the two types of duplexes. In addition to the shallow dependence on bridge length, no apparent oscillatory periods on HT yields were observed, indicating that step-wise hopping mechanism with small domains over adjacent base pairs as the hole carriers may not be dominant in hybrid duplex, similar as in B-form adenine tracts34.…”

Section: Resultsmentioning

confidence: 92%

Hole Transport in A-form DNA/RNA Hybrid Duplexes

Wong

Shao

2017

Sci Rep

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DNA/RNA hybrid duplexes are prevalent in many cellular functions and are an attractive target form for electrochemical biosensing and electric nanodevice. However the electronic conductivities of DNA/RNA hybrid duplex remain relatively unexplored and limited further technological applications. Here cyclopropyl-modified deoxyribose- and ribose-adenosines were developed to explore hole transport (HT) in both DNA duplex and DNA/RNA hybrids by probing the transient hole occupancies on adenine tracts. HT yields through both B-form and A-form double helixes displayed similar shallow distance dependence, although the HT yields of DNA/RNA hybrid duplexes were lower than those of DNA duplexes. The lack of oscillatory periods and direction dependence in HT through both helixes implied efficient hole propagation can be achieved via the hole delocalization and coherent HT over adenine tracts, regardless of the structural variations.

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Section: Resultsmentioning

confidence: 92%

Hole Transport in A-form DNA/RNA Hybrid Duplexes

Wong

Shao

2017

Sci Rep

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“…Discotic molecule PyTp and PyTp‐DNA complex are rich in π‐electrons. Furthermore, DNA has been shown to act as a bridge for electron conduction as well ,. The presence of π‐electrons in the medium between electrode and redox species, which is an LB film in the present case, will definitely promote the electron transfer process.…”

Section: Resultsmentioning

confidence: 77%

Electrochemical Charge Transfer Through the Supramolecular Discogen‐DNA Hybrid Multi‐layered Assembly

Pandey

Pandey²,

Nayak

2018

ChemistrySelect

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In the present work, charge transfer phenomenon through the films of an ionic discotic liquid crystal (discogen) that is pyridinium tethered with hexaalkoxytriphenylene (PyTp) and its complex with DNA (PyTp-DNA) was studied by using electrochemical methods. The PyTp and PyTp-DNA hybrid films were first formed at the air-water interface and then transferred onto the gold substrates by Langmuir-Blodgett (LB) technique. Electrochemical measurements carried out on these films in three different redox systems, namely, ferrocene, hexaammineruthenium (III), and ferrocyanide, revealed that the ferrocene and the hexaammineruthenium (III) systems allow the electron transfer reaction between redox probes and metal, whereas the ferrocyanide system completely blocks it. The electrochemical response of the films, in the former two systems, has been attributed to the bridge mediated electron transfer process, whereas, in the ferrocyanide system, the impermeability of redox ions through these LB films is responsible for the complete blocking of electron transfer. Based on the observed results, this work highlights the possibility of novel charge transfer properties of discogen-DNA type hybrid films for applications in molecular electronics.

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“…The results suggest that the DNAs complexed with ILs, C 4 mim, are less likely to form folded structure during nanopore analysis possibly due to the increased persistence length of DNA. The binding of ILs to the minor groove of double‐stranded DNA would transform the DNA into more rigid state . The possible conformation of DNA bound with C 4 mim is shown in Figure a.…”

Section: Resultsmentioning

confidence: 97%

“…The binding of ILs to the minor groove of double-stranded DNA would transform the DNA into more rigid state. [25,34] The possible conformation of DNA bound with C 4 mim is shown in Figure 2a. In molecular dynamic (MD) simulation, the bases in DNA strands were reported to be disturbed by the binding of ILs to the minor groove of DNA while the helical structure of double-stranded DNA is well maintained.…”

Section: Resultsmentioning

confidence: 99%

Reduction of DNA Folding by Ionic Liquids and Its Effects on the Analysis of DNA–Protein Interaction Using Solid‐State Nanopore

Jeong

Luo

Lim

et al. 2018

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DNA folding is not desirable for solid-state nanopore techniques when analyzing the interaction of a biomolecule with its specific binding sites on DNA since the signal derived from the binding site could be buried by a large signal from the folding of DNA nearby. To resolve the problems associated with DNA folding, ionic liquids (ILs), which are known to interact with DNA through charge-charge and hydrophobic interactions are employed. 1-n-butyl-3-methylimidazolium chloride (C mim) is found to be the most effective in lowering the incident of DNA folding during its translocation through solid-state nanopores (4-5 nm diameter). The rate of folding signals from the translocation of DNA-C mim is decreased by half in comparison to that from the control bare DNA. The conformational changes of DNA upon complexation with C mim are further examined using atomic force microscopy, showing that the entanglement of DNA which is common in bare DNA is not observed when treated with C mim. The stretching effect of C mim on DNA strands improves the detection accuracy of nanopore for identifying the location of zinc finger protein bound to its specific binding site in DNA by lowering the incident of DNA folding.

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Efficient DNA-Mediated Electron Transport in Ionic Liquids

Abstract: Engineering, American Chemical Society (ACS). It incorporates referee's comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.

Cited by 22 publications

References 77 publications

Hole Transport in A-form DNA/RNA Hybrid Duplexes

Hole Transport in A-form DNA/RNA Hybrid Duplexes

Electrochemical Charge Transfer Through the Supramolecular Discogen‐DNA Hybrid Multi‐layered Assembly

Reduction of DNA Folding by Ionic Liquids and Its Effects on the Analysis of DNA–Protein Interaction Using Solid‐State Nanopore

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