Efficient embryogenesis and regeneration in freshly isolated and cultured wheat (Triticum aestivum L.) microspores without stress pretreatment

Shariatpanahi, Mehran E.; Belogradova, K.; Hessamvaziri, Leila; Heberle‐Bors, Erwin; Touraev, Alisher

doi:10.1007/s00299-006-0205-7

Cited by 52 publications

(44 citation statements)

References 35 publications

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“…Exogenous application of PAs to anthers for 1 h appeared to exert a weak plant growth regulator-like effect on anthers with the formation of calli and regenerants having more adventitious roots. Shariatpanahi et al (2006) reported similar observations in cultured wheat microspore, in which the medium was supplemented with 1 mgl -1 2,4-D plus 0.2 mgl -1 kinetin. In this study, ELS or GRP/ 100 anthers was enhanced particularly with put, spd alone, or a combination of put, spd, or spm (Kelley et al 2002;Meijer and Simmonds 1988;Phillips et al 1987;Rajyalakshmi et al 1995).…”

Section: Discussionsupporting

confidence: 64%

Effects of exogenous application of polyamines on wheat anther cultures

Redha

Suleman

2010

Plant Cell Tiss Organ Cult

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Androgenesis of wheat genotypes was evaluated by pretreating anthers or embryo-like structures (ELS) with polyamines. Anthers of the genotype DH were pretreated with different concentrations of putrescine, spermidine, and spermine for 1, 3, and 6 h, and those of drought-tolerant International Center for Agricultural Research in the Dry Areas (ICARDA) wheat accessions were treated for 1 and 3 h. ELS of two genotypes were also treated for 30 and 60 min with the same polyamines and evaluated for green plant regeneration. The pretreatment of anthers with polyamines enhanced the development of ELS in all genotypes. The formation of ELS varied significantly with genotype. Pretreated anthers showed that four treatments improved significantly green plant regeneration with the genotype ICR 17. However, two treatments (1 mM putrescine or spermine for 1 h) significantly improved green plant regeneration per 100 ELS of only two ICAR-DA genotypes. ELS treated with polyamines for 30 min were greener and formed more adventitious roots. The chloroplasts of these greener ELS examined with a transmission electron microscope had agranal to grana thylakoids, while those of the control had plastids with mostly starch globules. Although exogenous application of polyamines to anthers improved the production of ELS and green plants, the effects of putrescine, spermidine, and spermine was dependent on genotype and the duration of pretreatment of anthers with the polyamines.

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Section: Discussionsupporting

confidence: 64%

Effects of exogenous application of polyamines on wheat anther cultures

Redha

Suleman

2010

Plant Cell Tiss Organ Cult

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“…Cold has been shown to repress the gametophytic pathway by inducing symmetrical microspore mitotic divisions and delaying pollen development (Shirdelmoghanloo et al, 2009). Cold shock is also thought to ensure the survival of a greater number of cells (Shariatpanahi et al, 2006). According to these authors, the understanding of an accurate mechanism of stress, with respect to microspore embryogenesis, is far to be elucidated, regardless of the chosen stress type (cold, heat, chemical or others).…”

Section: Resultsmentioning

confidence: 99%

“…Liu et al (2002) used in vitro selection in studies of resistance to pathogens, mineral imbalances, and drought tolerance. Moreover, this tool is also used in basic and advanced research (Germanà, 2011), such as genetic analysis, induction of mutation, genome mapping, gene transfer (Abdollahi et al, 2007), and QTL detection (Seymour et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…However, some conditions should be met for gametes to develop into a new plant. Stress pretreatments are considered crucial for triggering totipotency and activating the sporophytic pathway of the microspore cell (Shariatpanahi et al, 2006). The physiological conditions of donor plants and the microspore development stage are also very important because they can modify the androgenic response even in responsive genotypes (Germanà, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Poersch-Bortolon

Scagliusi

Lau

et al. 2016

Pesq. agropec. bras.

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-The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced more embryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.Index terms: Triticum aestivum, albinism, androgenesis, doubled haploid, isolated microspore culture, recalcitrance. Resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificanteResumo -O objetivo deste trabalho foi analisar a resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas, antes da cultura de micrósporos isolados, e ao efeito de um agente gelificante no meio de cultura de indução. Cinco genótipos foram avaliados quanto à formação de embriões, regeneração de plantas verdes e à duplicação espontânea dos cromossomos. Espigas de trigo foram submetidas a dois pré-tratamentos: frio, a 4°C por 21 dias; e ácido 2-hidroxinicotínico, a 32°C por dois dias. Os meios de cultura foram avaliados com ou sem Ficoll como agente gelificante. O frio produziu mais embriões e plantas verdes do que o pré-tratamento químico, em quatro dos cinco genótipos testados. Apenas dois genótipos tratados com ácido 2-hidroxinicotínico foram capazes de produzir plantas, e um deles produziu uma única planta albina. O meio com Ficoll produziu mais embriões do que o meio líquido e gerou maior número de plantas. A duplicação espontânea dos cromossomos varia entre os genótipos e os pré-tratamentos e apresenta alta variabilidade.Termos para indexação: Triticum aestivum, albinismo, androgênese, duplo-haploide, cultura de micrósporos isolados, recalcitrância.

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“…Even in 'recalcitrant' genotypes some AS were produced from the anthers excised from freshly cut tillers. Similarly, a method for producing DH wheat plants without any apparent stress treatment was described by Shariatpanahi et al (2006b). It could be supposed that for some genotypes accumulation of various types of stress factors (mechanical injuring, disinfection, transfer to in vitro culture conditions) during in vitro culture set-up procedure generates a sufficient signal to induce microspore reprogramming.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Żur

Dubas

Krzewska

et al. 2014

Plant Cell Tiss Organ Cult

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To verify the hypothesis that cell redox status regulates the process of microspore embryogenesis (ME), reactive oxygen species (ROS) generation and the activity of enzymatic and non-enzymatic antioxidants were analyzed in eight doubled haploid lines of triticale with significant differences in embryogenic potential. The analyses were performed in anthers excised from freshly cut tillers (control) and from low temperature (LT) pre-treated tillers (3 weeks at 4°C) in which ME has been initiated. Significant associations between ME effectiveness and the variables studied were found. In control cultures, high superoxide dismutase (SOD) activity appeared crucial for microspore viability. On the other hand, positive though non-linear correlation between ME effectiveness and H 2 O 2 generation, and negative correlation with catalase (CAT) activity suggest that some threshold level of H 2 O 2 is important for successful ME initiation. LT tillers pretreatment significantly increased H 2 O 2 accumulation, which had a negative effect on ME effectiveness. However, even high level of H 2 O 2 did not endanger cell viability as long as the cells exhibited high activity of ROS-decomposing enzymes (SOD, CAT and POX). The ability to sustain antioxidative enzyme activity under cold stress in the dark was another important requirement for high effectiveness of ME, allowing for the generation of the signal initiating microspore reprogramming and simultaneously protecting the cells from the toxic effects of ROS production. The role of antioxidative enzymes cannot be replaced even by high activity of non-enzymatic antioxidants. In conclusion, genetically controlled but environmentally modified cell tolerance to oxidative stress seems to play an important role in triticale ME.

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Efficient embryogenesis and regeneration in freshly isolated and cultured wheat (Triticum aestivum L.) microspores without stress pretreatment

Cited by 52 publications

References 35 publications

Effects of exogenous application of polyamines on wheat anther cultures

Effects of exogenous application of polyamines on wheat anther cultures

Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

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