Efficient ex-vitro rooting and acclimatization for tissue culture plantlets of ginger

Zhou, Jie; Guo, Fengling; Qi, Chuangdong; Fu, Jing; Xiao, Ying; Wu, Jinping

doi:10.1007/s11240-022-02296-3

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…Acclimatization of plantlets was performed first in plastic containers measuring 119 × 90 mm and 80 mm high and containing 150 g of a peat (75%) and perlite (25%) mix under controlled growth conditions (25 ± 1 °C and 16 h light and 8 h darkness) to promote the hardening of plantlets. The mix of peat and perlite used ensured good aeration of the root system, which is considered one of the key elements for the survival of tissue culture plantlets transferred from a lab to pots [ 72 ]. A plant rooted in Jiffy-7-Pellet ® with a length of 5 cm was placed inside each individual plastic container.…”

Section: Methodsmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L−1), Naphthaleneacetic Acid (NAA) (1 mg L−1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L−1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L−1) and NAA (1 mg L−1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L−1) and NAA (0.25, 0.50, and 1 mg L−1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L−1 of BAP and 1 mg L−1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L−1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

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Section: Methodsmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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“…Differences between the microenvironments of seedlings cultured in the lab with those in field leave regenerated plantlets with delicate and weakened root systems, which results in low survival rates of ginger [ 15 ]. In Medicago arborea L. study, an effective acclimatization project might enable the generation of high-quality seedlings to improve their survival rates [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Promoting the application of Pinus thunbergii Parl. to enhance the growth and survival rates of post-germination somatic plantlets

Sun

Wang

et al. 2023

BMC Plant Biol

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Objective There is a growing need for nematode resistant Pinaceae species plantlets to cope with the global scale degradation of coniferous forests, due to the prevalence of pine wilt disease. One of the bottlenecks that limits the commercialization of Pinaceae species plantlets is regeneration following their transfer from controlled sterile environments to the field while maintaining high survival rates. Methods The growth factors of somatic plantlets (SPs), such as sucrose, media, culture substrate, brassinolide and spectrum were investigated to promote the application of somatic nematode-resistant P. thunbergii plants in afforestation. Results The 1/2 WPM liquid medium, culture substrate (perlite and vermiculite =1:1), and carbohydrate (20 g/L sucrose) were effective in stimulating the growth of rooted SPs. While for unrooted SPs, 1 ug/L of brassinolide enhanced plantlet growth and rooting. And blue light (B) significantly promoted the longitudinal growth of shoots, while red light (R) was beneficial for root growth during the laboratory domestication stage. High quality SPs were obtained at a R/B ratio of 8:2. Following this acclimatization protocol, the P. thunbergii SPs could be directly transplanted to the field with a higher survival rate (85.20 %) in a forcing house. Conclusion this acclimatization protocol extremely improved the survival rate of P. thunbergii SPs. Moreover, this work will contribute to enhancing the possibilities for somatic plant afforestation with Pinus species.

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“…The transition from lab-to field-seedlings is a major obstacle in plant breed technology. The difference of microenvironment between seedlings cultured in lab and in field made the regeneration plant delicate and weakened root system which resulted in a survival rate (Zhou et al, 2022). Transplantation survival directly determines whether tissue-cultured regeneration plant can be utilized in field production.…”

Section: Introductionmentioning

confidence: 99%

Promoting the application of pinus thunbergii Parl. toward enhancing the growth and survival rates of post-germination somatic plantlets

Sun

Wang

et al. 2022

Preprint

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There is a growing need for nematode-resistant Pinaceae species seedlings to cope with the degradation of global coniferous forests due to the prevalence of pine wilt disease. One of obstacle of Pinaceae species plantlets commercialization is the regeneration plantlets from a controlled, sterile environment to the field while maintaining a high survival rate. The low survival rate of plantlets transferred from a lab to soil is mainly because of the quality of plantlet which makes commercialization challenging. For this paper, we investigated the factors that influence the growth of post-germination somatic regeneration plants (SRPs) to promote the application of nematode-resistant of Pinus thunbergii. The results indicated that, for rooted plantlet, a suitable liquid medium (1/2 WPM), transplantation stroma (perlite and vermiculite =1:1), and carbohydrate (20 g/L sucrose) were effective for promoting the growth of SRPs. While, for unrooted plantlets, 1 ug/L of brassinolide enhanced plantlets growth and rooting. In the laboratory domestication stage, under light spectrum treatments, blue light (B) significantly promoted the longitudinal growth of shoots, while red light (R) was of benefit for the improvement of root growth. The optimal combination of R/B was the 8:2. After this domestication culture, SRPs of Pinus thunbergii could be directly transplanted to the field with a higher survival rate (85.2%). This is the first report on domestication of conifer regeneration plants, that will increase the possibility of afforestation of Pinaceae or other woody plants. In addition, it contributes to breeding project of pine resistance to pine wilt disease.

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Efficient ex-vitro rooting and acclimatization for tissue culture plantlets of ginger

Cited by 9 publications

References 19 publications

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Promoting the application of Pinus thunbergii Parl. to enhance the growth and survival rates of post-germination somatic plantlets

Promoting the application of pinus thunbergii Parl. toward enhancing the growth and survival rates of post-germination somatic plantlets

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