Efficient gene expression in megakaryocytic cell line using nucleofection

Isakari, Yoshimasa; Harada, Yasuo; Ishikawa, Dai; Matsumura-Takeda, Kuniko; Sogo, Shinji; Ishida, Tatsuhiro; Taki, Takao; Kojima, Hiroshi

doi:10.1016/j.ijpharm.2007.01.042

Cited by 7 publications

(7 citation statements)

References 24 publications

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“…Recently, we established a gene-transfer system for MEG-01 cells. 23) These powerful tools, including the gene delivery system and this comprehensive gene expression analysis of PMA-treated MEG-01 cells, will help future studies of, and search for, novel molecules related to PLT-production.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Analysis during Platelet-Like Particle Production in Phorbol Myristate Acetate-Treated MEG-01 Cells

Isakari

Sogo

Ishida

et al. 2009

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 99%

Gene Expression Analysis during Platelet-Like Particle Production in Phorbol Myristate Acetate-Treated MEG-01 Cells

Isakari

Sogo

Ishida

et al. 2009

Biological & Pharmaceutical Bulletin

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“…Previous studies reported MEG-01 as a difficult cell line to transfect; consequently, the amount of labeled-RNA was limited. 32 Confocal microscopy showed that labeled RNA was localized outside and inside the …”

Section: Rna Transfer From Meg-01 To Huvecs and Thp-1 Cellsmentioning

confidence: 99%

Platelets and platelet-like particles mediate intercellular RNA transfer

Risitano

Beaulieu

Vitseva

et al. 2012

Blood

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“…Various chemical, physical or virus-based transfection and transduction strategies were developed [1], [2]. Dependent on type and/or cell cycle state of the target cell population, these techniques might provide low transfer efficacies, be not suitable for non-adherent cells, require toxic reagents, give limited access to the nucleus, or are time-consuming [3], [4], [5], [6].…”

Section: Introductionmentioning

confidence: 99%

Combined Pulse Electroporation – A Novel Strategy for Highly Efficient Transfection of Human and Mouse Cells

et al. 2010

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The type of a nucleic acid and the type of the cell to be transfected generally affect the efficiency of electroporation, the versatile method of choice for gene regulation studies or for recombinant protein expression. We here present a combined square pulse electroporation strategy to reproducibly and efficiently transfect eukaryotic cells. Cells suspended in a universal buffer system received an initial high voltage pulse that was continuously combined with a subsequent low voltage pulse with independently defined electric parameters of the effective field and the duration of each pulse. At comparable viable cell recoveries and transfection efficiencies of up to 95% of all cells, a wide variety of cells especially profited from this combined pulse strategy by high protein expression levels of individual cells after transfection. Long-term silencing of gene expression by transfected small interfering RNA was most likely due to the uptake of large nucleic acid amounts as shown by direct detection of fluorochromated small interfering RNA. The highly efficient combined pulse electroporation strategy enables for external regulation of the number of naked nucleic acid molecules taken up and can be easily adapted for cells considered difficult to transfect.

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Efficient gene expression in megakaryocytic cell line using nucleofection

Cited by 7 publications

References 24 publications

Gene Expression Analysis during Platelet-Like Particle Production in Phorbol Myristate Acetate-Treated MEG-01 Cells

Gene Expression Analysis during Platelet-Like Particle Production in Phorbol Myristate Acetate-Treated MEG-01 Cells

Platelets and platelet-like particles mediate intercellular RNA transfer

Combined Pulse Electroporation – A Novel Strategy for Highly Efficient Transfection of Human and Mouse Cells

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