Efficient generation of transgenic barley: The way forward to modulate plant–microbe interactions

Hensel, Goetz; Valkov, Vladimir Totev; Middlefell-Williams, Jill; Kumlehn, Jochen

doi:10.1016/j.jplph.2007.06.015

Cited by 122 publications

(98 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EU161573 (plants 'BG284') or pIPKb027 (plants 'BG353') (Himmelbach et al 2007). Agrobacterium-mediated plant transformation was performed as described elsewhere (Hensel et al 2008;Himmelbach et al 2007). …”

Section: Tillingmentioning

confidence: 99%

A Distorted Circadian Clock Causes Early Flowering and Temperature-Dependent Variation in Spike Development in the Eps-3Am Mutant of Einkorn Wheat

et al. 2014

Self Cite

View full text Add to dashboard Cite

Viable circadian clocks help organisms to synchronize their development with daily and seasonal changes, thereby providing both evolutionary fitness and advantage from an agricultural perspective. A high-resolution mapping approach combined with mutant analysis revealed a cereal ortholog of Arabidopsis thaliana LUX ARRHYTHMO/PHYTOCLOCK 1 (LUX/PCL1) as a promising candidate for the earliness per se 3 (Eps-3A m ) locus in einkorn wheat (Triticum monococcum L.). Using delayed fluorescence measurements it was shown that Eps-3A m containing einkorn wheat accession KT3-5 had a distorted circadian clock. The hypothesis was subsequently confirmed by performing a time course study on central and output circadian clock genes, which showed arrhythmic transcript patterns in KT3-5 under constant ambient conditions, i.e., constant light and temperature. It was also demonstrated that variation in spikelet number between wild-type and mutants is sensitive to temperature, becoming negligible at 25°. These observations lead us to propose that the distorted clock is causative for both early flowering and variation in spike size and spikelet number, and that having a dysfunctional LUX could have neutral, or even positive, effects in warmer climates. To test the latter hypothesis we ascertained sequence variation of LUX in a range of wheat germplasm. We observed a higher variation in the LUX sequence among accessions coming from the warmer climate and a unique in-frame mutation in early-flowering Chinese T. turgidum cultivar 'Tsing Hua no. 559.' Our results emphasize the importance of the circadian clock in temperate cereals as a promising target for adaptation to new environments.T HE circadian clock is an intrinsic regulator of biological processes oscillating within an $24-hr period (Pittendrigh 1993). It is considered to be the main mechanism by which plants recognize the optimal photoperiod for seasonal flowering (Imaizumi 2009). Transcriptional regulation of the circadian clock has been well described in Arabidopsis (Pokhilko et al. 2012) with the latest model emphasizing the importance of the Evening Complex (EC) composed of EARLY FLOWERING 3, EARLY FLOWERING 4, and LUX AR-RHYTHMO/PHYTOCLOCK 1 (ELF3, ELF4, and LUX/PCL1) proteins (Onai and Ishiura 2005;Nusinow et al. 2011;Pokhilko et al. 2012). The EC directly represses the function of PSEUDO RESPONSE REGULATOR 9 (PRR9) and acts antagonistically to the elements expressed in the morning, including LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) (Pokhilko et al. 2012). LHY, CCA1, and PRR9 form the so-called morning loop, which becomes arrhythmic when the EC is impaired (Hazen et al. 2005; Dixon et al. 2011; Nusinow Copyright © 2014 Dixon et al. 2011;Pokhilko et al. 2012). Genetic studies have shown that the recently cloned maturity-a, (mat-a; syn. early maturity 8, eam8) locus is an ortholog of AtELF3 in barley (Hordeum vulgare L.). eam8 appears to be epistatic to eam10 (syn. ea sp ), which in turn seems to be a possible ortholog of LUX/PCL...

show abstract

Section: Tillingmentioning

confidence: 99%

A Distorted Circadian Clock Causes Early Flowering and Temperature-Dependent Variation in Spike Development in the Eps-3Am Mutant of Einkorn Wheat

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, transformation efficiency remains still variable and rather genotype dependent. In barley, the most readily transformed cultivar is 'Golden Promise', which allows an average of >10 independent transformation events per immature embryo (Bartlett et al, 2008;Hensel et al, 2009;Murray et al, 2004); other cultivars, while being amenable to transformation, show a lower level of efficiency (Hensel et al, 2008;Murray et al, 2004). One suggested means of overcoming this genotype dependency was to replace immature embryos with isolated ovules as the recipient tissue.…”

Section: Agrobacterium-mediated Gene Transfermentioning

confidence: 99%

Genetic Transformation of Triticeae Cereals for Molecular Farming

Hensel¹

2011

Genetic Transformation

Self Cite

View full text Add to dashboard Cite

“…Current barley transformation protocols claim an efficiency of over 10% (i.e. ten independent transgenic regenerants per 100 inoculated embryos) (Matthews et al 2001, Coronado et al 2005, Hensel et al 2008), a significant advance over the rate achieved by biolistic transformation. However, the efficiency of current Agrobacterium-based methods achievable in wheat (Wu et al 2003, Hu et al 2003), cereal rye and triticale (Nadolska-Orczyk et al 2005) remains comparable to the results obtained by biolistic transformation.…”

Section: Immature Embryos and Derived Callusmentioning

confidence: 99%

“…In later studies however, it was found that the selection systems based on the Streptomyces spec. phosphinothricin acetyltransferase genes pat and bar conferring resistance to the herbicide Phosphinothricin (barley: Wan and Lemaux 1994, Tingay et al 1997, Horvath et al 2000, wheat: Becker et al 1994, Nehra et al 1994, Wu et al 2003 as well as on the E. coli hygromycin phosphotransferase (HPT) gene which confers resistance to the antibiotic Hygromycin B (barley: Matthews et al 2001, Coronado et al 2005, Hensel et al 2008, wheat: Hensel et al 2009) are more effective. The 5-enol-pyruvyl shikimate-3-phosphate synthase (EPSPS) encoded by the aroA gene from Agrobacterium strain CP4 confers resistance to the non-selective herbicide Glyphosate.…”

Section: Selectable Markersmentioning

confidence: 99%

“…For example, candidate genes anticipated to confer tolerance to abiotic stress or resistance to a pathogen can best be analysed in the context of a susceptible plant genetic background. In surveys of both wheat and barley germplasm, several elite lines have proven amenable to transformation, albeit often at the cost of transformation efficiency (Takumi and Shimada 1997, Wu et al 2003, Hensel et al 2008. In particular, the German winter wheat cultivar "Certo" appears relatively easy to transform both via biolistic (Varshney and Altpeter 2001) or via gene transfer by means of Agrobacterium .…”

Section: Transformation Efficiency Is Dependent On the Recipient's Gementioning

confidence: 99%

See 1 more Smart Citation

Genetic transformation technology in the Triticeae

Kumlehn

Hensel

2009

Breed. Sci.

Self Cite

View full text Add to dashboard Cite

The Triticeae crops comprise wheat, barley, rye and triticale, which together provide a major portion of the world's food and feed. The growing demand for human nutrition and renewable energy requires an intensification in application-oriented research and the establishment and utilization of current biotechnology in these crops. Genetic transformation provides an important means both to elucidate gene function, and to engineer crop plants in a directed and precise way. This review covers a range of issues surrounding the production of stable transgenic lines within the Triticeae. Some quality aspects of transgenesis of particular relevance to the Triticeae are also discussed.

show abstract

Efficient generation of transgenic barley: The way forward to modulate plant–microbe interactions

Cited by 122 publications

References 46 publications

A Distorted Circadian Clock Causes Early Flowering and Temperature-Dependent Variation in Spike Development in the Eps-3Am Mutant of Einkorn Wheat

A Distorted Circadian Clock Causes Early Flowering and Temperature-Dependent Variation in Spike Development in the Eps-3Am Mutant of Einkorn Wheat

Genetic Transformation of Triticeae Cereals for Molecular Farming

Genetic transformation technology in the Triticeae

Contact Info

Product

Resources

About