Efficient in vitro plant regeneration via indirect organogenesis for different common bean cultivars

Collado, Raúl; Veitía, Novisel; Bermúdez-Caraballoso, Idalmis; García, Leonardo; Torres, Damaris; Romero, Carlos; Lorenzo, José Luis Rodríguez; Angenon, Geert

doi:10.1016/j.scienta.2013.02.007

Cited by 17 publications

(20 citation statements)

References 30 publications

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“…On the other hand, another solution comprises using a regeneration system based on indirect organogenesis with a callus phase, which shoot may arise from a single cells as was described before for P. acutifolious (Zambre et al 2005) and Glycine max (Hong et al 2007). This is further supported by recent results in our lab that green nodular calli showed high regeneration capability (Collado et al 2013) and they were also susceptible to Agrobacterium inoculation (data not shown).…”

Section: Discussionsupporting

confidence: 58%

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Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Collado

Bermúdez-Caraballoso

García

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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Common bean is recalcitrant to genetic transformation, due to limited regeneration capacity and low DNA transfer rates. The effect of different parameters on T-DNA transfer from Agrobacterium tumefaciens, was studied by measuring transient expression of the β-glucuronidase gene in Phaseolus vulgaris L. cv. CIAP7247F. Epicotyl containing seedling explants were inoculated with Agrobacterium EHA101 and C58C1Rif R (pMP90) strains harboring the binary vector pTJK136 with GusA gene on the T-DNA. Parameters studied were temperature and light regime during co-cultivation, explant injury, and the acetosyringone concentration for vir gene induction. The co-cultivation temperature and photoperiod had a significant effect on Agrobacterium DNA transfer. In addition, explant injury and supplementation of the cocultivation medium with acetosyringone increased the GUS activity. Optimal T-DNA transfer was obtained under the following conditions: co-cultivation at 25°C in darkness, injuring the explants with carborundum, and supplementation of the co-cultivation medium with 200 μM acetosyringone. This T-DNA delivery system was combined with a direct organogenesis protocol using epicotyl explants and fertile regenerants were recovered from tissue transformed with Agrobacterium. However, no transmission of transgenes to progeny could be observed, suggesting that the obtained plants were chimeras.

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Section: Discussionsupporting

confidence: 58%

“…CIAP7247F, a highyield cultivar widely used in Cuba, were disinfected and sowed in vitro (Fig. 1a) on germination medium (GM, Table 1) as described in Collado et al (2013).…”

Section: Methodsmentioning

confidence: 99%

Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Collado

Bermúdez-Caraballoso

García

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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“…Las semillas se desinfectaron previo a la siembra mediante inmersión en etanol al 70% durante 10 segundos seguido de una solución de hipoclorito de sodio al 1.5% durante 10 min y por último se lavaron tres veces con agua desionizada para eliminar cualquier residuo de desinfectante (Collado et al, 2013). Seguidamente las semillas fueron colocadas en papel f iltro para su secado y posteriormente fueron embebidas durante 30 min con el tratamiento homeopático correspondiente o con agua destilada en el caso del tratamiento control.…”

Section: Experimental Developmentunclassified

“…Seeds were disinfected previous to sowing by immersion in ethanol at 70% for 10 s followed by a sodium hypochlorite 1.5% solution for10 min and f inally washed thrice with deionized water to eliminate any disinfectant residual (Collado et al, 2013). Subsequently, seeds were placed on f ilter paper for drying and then soaked in the corresponding homeopathic treatment or with distilled water in the case of the control treatment for 30 min.…”

Section: Variables Morfométricasmentioning

confidence: 99%

La Homeopatía incrementa la tolerancia al estrés por NaCl en plantas de frijol común (Phaseolus vulgaris L.) variedad Quivicán

Mazón‐Suástegui¹,

Ojeda-Silvera²,

García-Bernal

et al. 2020

Terra

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“…Successful plant regeneration was demonstrated using roots as explants in Brassicas (Sharma and Thorpe, 1989;Wong and Loh, 1988). Cotyledonary node explants were used to regenerate shoots, mainly in species of legume plants including dry bean (Phaseolus vulgaris L.) (McClean and Grafton, 1989), lentil (Lens culinaris) (Bermejo et al, 2012;Chhabra et al, 2008;Warkentin and McHughen, 1993), mung bean (Vigna raditat L. Wilczek) (Gulati and Jaiwal, 1994), and common bean (P. vulgaris) (Arellano et al, 2009;Collado et al, 2013). However, no study to date has reported successful and efficient in vitro propagation and plant regeneration from hypocotyls, cotyledonary nodes, and radicles of C. oleifera.…”

mentioning

confidence: 99%

In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

Liu

Lin

et al. 2016

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Camellia oleifera Abel. is one of four major woody oil plants in the world. The objective of the current study was to evaluate the effect of different plant growth regulators (PGRs) and concentrations on direct organogenesis using cotyledonary nodes, hypocotyls, and radicle explants. High induction frequency of adventitious shoots were obtained from cotyledonary nodes, hypocotyls, and radicle explants (85.2%, 73.6%, and 41.0%, respectively) when cultured on half-strength Murashige and Skoog (1/2 MS) medium containing 2.0 mg·L−1 6-benzylaminopurine (BA) and 0.1 mg·L−1 indole-3-acetic acid (IAA). Microshoots from cotyledonary nodes, hypocotyls, and radicle explants were then transferred to 1/2 MS medium containing 2.0 mg·L−1 BA and 0.05 mg·L−1 indole-3-butyric acid (IBA) for shoot multiplication, resulting in 6.9 shoots per explant. The shoots were transferred to Woody Plant Medium (WPM) supplemented with various α-naphthalene acetic acid (NAA) and gibberellic acid (GA3) for shoot elongation. The mean length of shoots and the number of leaves per shoot were 3.7 and 6.6 cm, respectively, in WPM supplemented with 0.5 mg·L−1 NAA and 3.0 mg·L−1 GA3. The highest rooting of shoots (90.2%) or the number of roots per shoot (7.2) was obtained when elongated microshoots were transferred to 1/2 MS medium supplemented with 3.5% perlite, 1.0 mg·L−1 IBA and 2.0 mg·L−1 NAA. The rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90.0%. The in vitro plant regeneration procedure described in this study is beneficial for mass propagation and improvement of C. oleifera through genetic engineering.

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Efficient in vitro plant regeneration via indirect organogenesis for different common bean cultivars

Cited by 17 publications

References 30 publications

Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens

La Homeopatía incrementa la tolerancia al estrés por NaCl en plantas de frijol común (Phaseolus vulgaris L.) variedad Quivicán

In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

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