Efficient in vitro shoot bud proliferation from cotyledonary nodes and apical buds of Moringa oleifera Lam.

Zheng, Mingyang; Yang, Heyue; Yang, Endian; Zhou, Xuan; Chen, Xiaoyang; Zhang, Junjie

doi:10.1016/j.indcrop.2022.115394

Cited by 5 publications

(1 citation statement)

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“…A reasonable ratio of growth regulators is especially crucial for the induction and proliferation of adventitious shoots; thus, the formulas of the growth regulators in tissue culture systems vary among [41,42]. In the tissue culture process of woody plants, the commonly used plant growth regulators are TDZ, NAA, IBA, and kinetin [43,44]. However, different species have different degrees of sensitivity to plant growth regulators.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Efficient Regeneration of Rhododendron decorum from Flower Buds

Qian

et al. 2023

Horticulturae

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Rhododendron decorum is a woody species with high ornamental and medical value. Herein, we introduce a novel in vitro regeneration method for R. decorum. We used flower buds to develop an efficient and rapid plant regeneration protocol. Sterile flower buds of R. decorum of a 2 cm size were used as explants to study the effects of the culture medium and plant growth regulators on the callus induction and adventitious shoot differentiation, proliferation, and rooting. According to the results, the optimal medium combination for callus induction was WPM + 1 mg/L TDZ + 0.2 mg/L NAA, and its induction rate reached 95.08%. The optimal medium combination for adventitious shoot differentiation from the callus was WPM + 0.5 mg/L TDZ + 0.1 mg/L NAA, and its differentiation rate reached 91.32%. The optimal medium combination for adventitious shoot proliferation was WPM + 2 mg/L ZT + 0.5 mg/L NAA, for which the proliferation rate reached 95.32% and the proliferation coefficient reached 9.45. The optimal medium combination for rooting from adventitious shoots was WPM + 0.1 mg/L NAA + 1 mg/L IBA, and its rooting rate reached 86.90%. The survival rates of the rooted regenerated plantlets exceeded 90% after acclimatization and transplantation. This regeneration system has the advantages of being simple and highly efficient, and it causes little damage to the shoots of the mother plants, laying a foundation for the plantlet propagation, genetic transformation, and new-variety breeding of R. decorum.

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