Efficient in vivo catheter-based pericardial gene transfer mediated by adenoviral vectors

Abstract

Adenoviral vectors are promising agents for a number of in vivo gene therapy applications including diseases of the heart and coronary vessels. Efficient intravascular gene transfer to specific sites has been achieved in occluded vessels, but otherwise is hampered by the effect of blood flow on localized vector uptake in the vessel wall. An alternative delivery approach to coronary arteries is the expression of diffusible gene products into the pericardial space surrounding the heart and coronary arteries. However, in vivo pericardial access is comparatively difficult and has been limited to surgical approaches. We hypothesized that efficient adenovirus‐mediated gene expression in pericardial lining mesothelium could be achieved by transmyocardial vector delivery to the pericardium. To evaluate this concept, a hollow, helical‐tipped penetrating catheter was used to deliver vector‐containing fluid directly into the intrapericardial space. The catheter was introduced percutaneously in anesthetized mongrel dogs, advanced into the right ventricle, and the tip passed through the apical right ventricular myocardium under direct radiographic visualization until the open end of the catheter tip resided in the intrapericardial space. Adenoviral vectors expressing either nuclear‐localizing beta‐galactosidase, cytoplasmic luciferase, or secreted human α1AT reporters (Av1nBg, Av1Lu, or Av1Aa, respectively) were instilled through the catheter into the intrapericardial space. Three days later the animals were sacrificed and reporter gene expression was evaluated in pericardium, epicardium, and multiple other tissues. In animals receiving Av1nBg, beta‐galactosidase activity was evident in most of the pericardial lining endothelium, up to 100% in many areas. In animals receiving Av1Lu, luciferase reporter activity was abundant in pericardial tissues, but near‐background levels were observed in other organs. In animals receiving Av1Aa, human α1AT was abundant (16–29 mg/ml) in pericardial fluid, but was undetectable in serum. All animals tolerated the procedure well with no electrocardiographic changes and no clinical sequelae. These observations demonstrate highly efficient adenovirus vector delivery and gene transfer and expression in the pericardium and support the feasibility of localized gene therapy via catheter‐based pericardial approaches. We suggest that the pericardial sac may serve as a sustained‐release protein delivery system for the generation of desired gene products or their metabolites for diffusion into the epicardial region.

Augmentation of Intrapericardial Nitric Oxide Level by a Prolonged-Release Nitric Oxide Donor Reduces Luminal Narrowing After Porcine Coronary Angioplasty

“…This study confirms our initial description of the effectiveness of percutaneous instillation of material into a normal pericardial space with a helical needle-tipped catheter, 16,17 as well as by a transthoracic approach. 20 This method appears to be reasonably safe, with no evidence of adverse sequelae over several days after the procedure.…”

“…17 Briefly, a catheter inserted into the right jugular vein was advanced under fluoroscopic guidance into the right ventricle to the cardiac apex, then through the myocardium into the pericardial space. Proper catheter position was confirmed fluoroscopically by infusion of meglumine before flushing with 2 mL of saline.…”

Myocardial gene transfer by selective pressure-regulated retroinfusion of coronary veins

“…10,11 Although some focal areas of the epicardium were reached after pretreatment with doxycyclin, 11 the pericardial approach does not yet seem to be sufficient to provide myocardial gene expression with homogeneous transmural distribution.…”

Imaging of light emission from the expression of luciferases in living cells and organisms: a review

“…Using imaging of luciferase light emission, March et al (1999) showed the feasibility of catheter-based pericardial local delivery of adenoviral vectors for gene therapy in dogs (159). One of the recombinant adenoviral expression vectors encoded luciferase in the cytoplasm.…”