Efficient isolation of genes differentially expressed on cellulose by suppression subtractive hybridization in Agaricus bisporus

Morales, P.; Thurston, Christopher F.

doi:10.1017/s0953756203007366

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…Few EST of B. bassiana have been reported, and with relatively little research on the fungal genes and proteins it is not unexpected that many of the fungal genes found are novel. The novelty of the gene sequences is also consistent with previous SSH studies on fungal research that produced many unknown genes (Carpenter et al 2005, Morales andThurston 2003). However numerous genes involved in conidiation in the filamentous ascomycete A. nidulans have been identified and it is perhaps surprising that B. bassiana homologues were not found in this study.…”

Section: Discussionsupporting

confidence: 90%

Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization

Wu¹,

Ridgway²,

Carpenter³

et al. 2008

Mycologia

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The conidiation of the entomopathogenic fungus Beauveria bassiana (Hyphomycete) is a complex process that involves the stage-and cell-typespecific expression of hundreds of genes. The suppression subtractive hybridization method was used to target genes involved in conidiation. Seventeen genes were cloned that potentially were involved in conidia formation. Six of them demonstrated differential expression between conidial and vegetative cultures. Sequence analysis showed three cDNA fragments had similarity to known genes involved in either cellular metabolism or cell regulatory processes. The other cDNA fragments showed low or no similarity to any genes previously described. The fulllength cDNA and genomic sequence of a gene designated A43 was isolated. The A43 protein is composed of 180 amino acids and has 34% identity to a RNA-binding region-containing protein. The temporal expression pattern was consistent with the gene being involved in conidiation. The colony morphology of the A43 knock-out mutant had more floccus mycelium than the wild-type and also produced fewer conidia, indicating the A43 gene is involved in B. bassiana conidiation.

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Section: Discussionsupporting

confidence: 90%

Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization

Wu¹,

Ridgway²,

Carpenter³

et al. 2008

Mycologia

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“…SSH has been shown by several investigations [19][20][21] as one of the efficient approaches to study gene expression in closely related subpopulations of cells in which equally expressed transcripts are removed during the process of hybridization. 15 However, one of the major difficulties in performing SSH in enriched HSPCs is the inability to obtain a sufficient number of cells to generate an adequate amount of cDNA using standard procedures.…”

Section: Derived From Fb-and Mpb-hspcsmentioning

confidence: 99%

Differential gene expression of human stem progenitor cells derived from early stages of in utero human hematopoiesis

Shojaei

Gallacher

Bhatia

2004

Blood

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“…Other authors have also used a similar approach in the investigation of pathogenic fungi [23,24]. There are few published studies related to the application of ESTs to identify genes expressed during fungal development and at different culture conditions [40,41]. Tagu and Martin [42] were the first to use this technology in studying plant–fungal interactions.…”

Section: Discussionmentioning

confidence: 99%

Identification ofHeterobasidion annosum(S-type) genes expressed during initial stages of conidiospore germination and under varying culture conditions

Abu

Asiegbu

2004

FEMS Microbiology Letters

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Expressed sequence tag analysis (EST) is a robust approach for detecting novel and potentially important genes during fungal development. The technique was used in this study to identify and characterise genes induced during spore germination and hyphal morphogenesis under different culture conditions. For this study, a cDNA library was constructed from the germinated spores of S-type of Heterobasidion annosum. In total, 12,288 clones of the H. annosum germinated spores (HAGS) cDNA library were arrayed on a high-density nylon membrane filter. A total of 1248 trimmed ESTs were randomly selected and sequenced. These ESTs were composed of 343 contigs from a total of 883 EST sequences. The BLASTX analysis of these sequenced clones revealed several kinds of genes with diverse functions (e.g., MAP kinase and endoglucanase, etc.). Differential hybridization assay on the HAGS cDNA library using cDNA probes made from H. annosum pre-grown under different culture conditions, such as 1.0% glucose, 0.01% ferulic acid or 0.01% oxalic acid revealed 20, 37, 44 positive hybridizing clones, respectively. Among the positively hybridizing clones 15 gene clones were common to all conditions whereas 4, 9 and 15 clones were unique in glucose, ferulic acid and oxalic acid medium, respectively. BLASTX analysis of the differentially expressed clones led to the identification of genes with significant homologue to cytochrome P450 monooxygenase and transcript antisense to ribosomal RNA. Northern analyses confirmed that expression of both genes were elevated in ferulic acid and oxalic acid culture conditions than in glucose medium. The enhanced accumulation of the gene transcripts at the different culture conditions is discussed with respect to their potential role during fungal morphogenesis and in planta growth.

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Efficient isolation of genes differentially expressed on cellulose by suppression subtractive hybridization in Agaricus bisporus

Cited by 14 publications

References 23 publications

Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization

Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization

Differential gene expression of human stem progenitor cells derived from early stages of in utero human hematopoiesis

Identification ofHeterobasidion annosum(S-type) genes expressed during initial stages of conidiospore germination and under varying culture conditions

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