Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol

Brundrett, Mark; Kendrick, Bryce; Peterson, Carol A.

doi:10.3109/10520299109110562

Cited by 547 publications

(343 citation statements)

References 14 publications

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“…Hand sections were stained with Toluidine blue and mounted on glass slides by using 15% Mowiol͞35% glycerol͞ 0.07 M Tris (pH 7.5). Selected sections were also stained with Sudan red 7B for neutral lipids (16).…”

Section: Methodsmentioning

confidence: 99%

sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein

Shen

Zhao

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

131

109

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A microscopy-based screen of a large collection of maize Mutator (Mu) transposon lines identified the supernumerary aleurone layers 1-1 (sal1-1) mutant line carrying up to seven layers of aleurone cells in defective kernel endosperm compared with only a single layer in wild-type grains. Normal, well filled endosperm that is homozygous for the sal1-1 mutant allele contains two to three layers of aleurone cells. Cloning of the sal1 gene was accomplished by using Mu tagging, and the identity of the cloned gene was confirmed by isolating an independent sal1-2 allele by reverse genetics. Homozygous sal1-2 endosperm has two to three layers of aleurone cells in normal, well filled grains. In situ hybridization experiments reveal that the sal1 gene is ubiquitously expressed in vegetative as well as zygotic grain tissues, with no difference being detected between aleurone cells and starchy endosperm cells. Northern blot analysis failed to detect the sal1-2 transcript in leaves of homozygous plants, suggesting that the allele is a true sal1 knockout allele. The sal1 gene encodes a homologue of the human Chmp1 gene, a member of the conserved family of the class E vacuolar protein sorting genes implicated in membrane vesicle trafficking. In mammals, CHMP1 functions in the pathway targeting plasma membrane receptors and ligands to lysosomes for proteolytic degradation. Possible roles for the function of the sal1 gene in aleurone signaling, including a defect in endosome trafficking, are discussed.

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Section: Methodsmentioning

confidence: 99%

sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein

Shen

Zhao

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

131

109

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“…Some of these seed sections were stained with 1% phloroglucinol (w/v) in 12% HCl for 5 min and observed with a bright field light microscope for the detection of lignin (Fernandez and Heath, 1986). Another set of cryostat sections were stained with Sudan III for the detection and localization of lipids under bright field microscopy (Brundrett et al, 1991). The remainder of the Cryostat sections were stained with Fluorol Yellow 088 and viewed with a fluorescent microscope Leica DM RB Fluo in the range of 515-565 nm (green) to detect lipids (Brundrett et al, 1991).…”

Section: Sectioning and Staining For Light And Fluorescence Microscopymentioning

confidence: 99%

“…Another set of cryostat sections were stained with Sudan III for the detection and localization of lipids under bright field microscopy (Brundrett et al, 1991). The remainder of the Cryostat sections were stained with Fluorol Yellow 088 and viewed with a fluorescent microscope Leica DM RB Fluo in the range of 515-565 nm (green) to detect lipids (Brundrett et al, 1991). Series of images were treated with the python program ALLAM-ODA2.0 (Papini, 2012) to reduce noise.…”

Section: Sectioning and Staining For Light And Fluorescence Microscopymentioning

confidence: 99%

The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy

Mosti

Friedman

Piccolin

et al. 2012

Flora - Morphology, Distribution, Functional Ecology of Plants

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Please cite this article in press as: Mosti, S., et al., The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy. Flora (2012) With light, fluorescence, transmission electron, and environmental scanning electron microscopy we studied the development of the Lunaria annua L. (Brassicaceae) seeds in order to reveal basic anatomical information about the unusual tissues of these seeds. In particular the seed tegument tissues possess complex morphological aspects that are relevant to the biology and ecology of this plant. A sclerenchymatic tissue as the innermost layer of the teguments apparently offers robust protection for the embryo, yet is organized to be flexible. This tissue likely controls the passage of water from the tegumental layers towards the embryo. We report here the presence of tannins in the pre-sclerenchymatic layer of the unripe seed. The inner tegument also houses a spongy tissue with wide intercellular spaces. This tissue could impart buoyancy to the seeds, which possibly might be required for water transport. The structural features could indicate that Lunaria may have evolved in a Mediterranean environment, which is characterized by a long dry season, but with a large amount of rainfall concentrated in short periods. Probably, not only the typical enlarged and flattened fruits of Lunaria can easily float and be dispersed away from the mother plant, but also the seeds have this dispersal peculiarity after release from the silicules.

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“…Sudan Red 7B (Sigma) was prepared as a 0.05% (w/v) solution in PEG400:glycerol (1:1 [v/v]; Brundrett et al, 1991). Soil-grown Arabidopsis roots were stained in this solution for 1 h at room temperature, rinsed briefly in distilled water, and free-hand sections were made at the base of the roots with a razor blade.…”

Section: Sudan Red 7b Staining and Microscopymentioning

confidence: 99%

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

et al. 2007

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The interface between plants and the environment is provided for aerial organs by epicuticular waxes that have been extensively studied. By contrast, little is known about the nature, biosynthesis, and role of waxes at the root-rhizosphere interface. Waxes isolated by rapid immersion of Arabidopsis (Arabidopsis thaliana) roots in organic solvents were rich in saturated C18-C22 alkyl esters of p-hydroxycinnamic acids, but also contained significant amounts of both a-and b-isomers of monoacylglycerols with C22 and C24 saturated acyl groups and the corresponding free fatty acids. Production of these compounds in root waxes was positively correlated to the expression of sn-glycerol-3-P acyltransferase5 (GPAT5), a gene encoding an acyltransferase previously shown to be involved in aliphatic suberin synthesis. This suggests a direct metabolic relationship between suberin and some root waxes. Furthermore, when ectopically expressed in Arabidopsis, GPAT5 produced very-longchain saturated monoacylglycerols and free fatty acids as novel components of cuticular waxes. The crystal morphology of stem waxes was altered and the load of total stem wax compounds was doubled, although the major components typical of the waxes found on wild-type plants decreased. These results strongly suggest that GPAT5 functions in vivo as an acyltransferase to a glycerol-containing acceptor and has access to the same pool of acyl intermediates and/or may be targeted to the same membrane domain as that of wax synthesis in aerial organs.

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Efficient Lipid Staining in Plant Material with Sudan Red 7B or Fluoral Yellow 088 in Polyethylene Glycol-Glycerol

Cited by 547 publications

References 14 publications

sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein

sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein

The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

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