Efficient micropropagation and chlorocholine chloride induced stevioside production of Stevia rebaudiana Bertoni

Abstract

A promising method of micropropagation of Stevia rebaudiana Bertoni has been developed with an aim to increase the biomass, survivability of the plantlets and stevioside production, using chlorocholine chloride (CCC). Microshoots transferred to the MS medium containing different combinations CCC and IBA were found to be most effective in terms of growth pattern, hardening ability of the plantlets and stevioside content, compared to MS medium containing either IBA or CCC. Among other combinations tested, MS medium supplemented with 3 mg/l CCC and 3 mg/l IBA was found most effective in inducing significant changes like reduced shoot length, increased number of roots, higher leaf size, increased biomass and chlorophyll retaining capacity, higher survival percentage and most importantly the elevated stevioside content. Collectively, the major observations of this research indicate that application of CCC in micropropagation of S. rebaudiana Bertoni is a promising approach and has commercial prospects.

Biotechnological approaches in Stevia rebaudiana and its Therapeutic Applications

“…2, 4-D with different concentrations reported lower callus induction on MS medium [29]. Dey et al (2013) was detected application of chlorocholine chloride (CCC) in micropropagation of S. rebaudiana Bertoni has commercial prospects [30]. The shoots were successfully rooted with addition auxin at concentration of 0.4 mg/l IBA, IAA or NAA [31].…”

“…Fatima and Khan (2010) improved the micropropagation method for Stevia rubadiana by incorporation of 1.5 mg/l BA + 0.5 mg/l Kn concentration for shoot induction from apical meristem and nodal explants [35]. Microshoots used as explant in investigated by Dey et al (2013) that MS medium containing different combinations chlorocholine chloride (CCC) and IBA compared to MS medium containing either IBA or CCC, MS medium supplemented with 3 mg/l CCC and 3 mg/l IBA reported most effective [30]. The direct shoot organogenesis with TDZ-Induced efficient on explants in in vitro for Sevia rebaudiana Bert.…”

Role of endomycorrhizae and Pseudomonas fluorescens on the acclimatization of micropropagated Stevia rebaudiana Bert. plantlets

“…On the other hand, propagation through seeds produces heterogeneous plants, resulting in variations in sweetening levels. Propagation by cutting is also limited, because of a lower number of individuals that can be obtained simultaneously from a single plant (Dey et al, 2013). Tissue culture techniques hold great promise for micropropagation, and enhancement the natural levels of in vitro Stevia plant (Jitendra et al, 2012).…”

An Efficient Protocol Of Stevia rebaudiana Regeneration For Large-Scale Production

“…In the past, a few tissue culture methods have been presented on S. rebaudiana plants using shoot tip, leaf, nodal and intermodal segments (Patil et al, 1996;Sivaram & Mukundan, 2003;Uddin et al, 2006;Debnath, 2008;Abd Alhady, 2011;Thiyagarajan & Venkatachalam, 2012;Aman et al, 2013 andDey et al, 2013).…”