Efficient optimization of ultra‐high‐ performance supercritical fluid chromatographic separation of Rosa sericea by response surface methodology

Abstract

An approach for rapid optimization of ultra-high-performance supercritical fluid chromatographic (UHPSFC) gradient by response surface methodology was developed for fast separation of complex crude extracts of the leaves of Rosa sericea. The optimization was performed with Box-Behnken designs and the multicriteria response variables were described using Derringer's desirability. Based on factorial design experiments, five factors were selected for Box-Behnken designs to optimize the UHPSFC conditions, which led to 46 experiments being performed within 8 h. An evaporative light-scattering detector (ELSD) was used, and quantitative analysis of main components in R. sericea samples was employed to evaluate the statistical significance of the parameters on UHPSFC-ELSD analytes response. The results indicated that the optimized UHPSFC-ELSD method is very sensitive with LODs and LOQs below 1.19 and 4.55 μg/mL, respectively. The overall intra- and interday variations were less than 3.91 and 6.41%, respectively. The recovery of the method ranged from 95.66 to 104.22%, with RSD < 5.91%. This newly developed UHPSFC-ELSD method was demonstrated to be fast and sensitive in analyzing complex herbal extracts of Traditional Chinese Medicines.

Extraction optimization by response surface methodology: Purification and characterization of phytosterol from sugarcane (Saccharum officinarum L.) rind

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Simultaneous determination of 16 polycyclic aromatic hydrocarbons in reclaimed water using solid‐phase extraction followed by ultra‐performance convergence chromatography with photodiode array detection

“…The aim of screening column is that all the analytes could be eluted and gets high resolutions with good peak shapes in short time. Although there are some reports about using SFC to separate mixed organic compounds , the theory about how to screen the column according to the sample properties is still developing. There is no detailed guide for choosing the stationary phase in SFC.…”

“…In addition, SFC shows specific selectivity for the similar structure compounds by addition of typical organic modifiers used in reversed‐phase chromatography to CO 2 and diverse range of stationary phases . With the introduction of a new generation of SFC with ultra‐performance convergence chromatography (UPC 2 ), the analysis time and the usage of organic solvent could be drastically reduced . The fast and high‐throughput characteristics of UPC 2 provide the possibility to analyze large amount of water samples in a limited time.…”

Triacylglycerols composition analysis of olive oils by ultra‐performance convergence chromatography combined with quadrupole time‐of‐flight mass spectrometry

“…Recently, ultra-performance convergence chromatography (UPC 2 ), a new generation of supercritical fluid chromatography (SFC), has been developed and shown to have a better separation of non-polar compounds, with reduced analytical time and organic solvent usages (Ruiz-Sambl as et al, 2010;Gourmel et al, 2013;Li et al, 2013). UPC 2 combined with quadrupole time-of-flight mass spectrometry (Q-TOF-MS) has been shown to serve as a powerful tool in investigating triacylglycerol compositions of sunflower, corn and soybean oils by providing accurate mass data and MS 2 fragment ion patterns after improved peak resolution (Zhou et al, 2014;Gao et al, 2017).…”