Efficient photoacoustic imaging using indocyanine green (ICG) loaded functionalized mesoporous silica nanoparticles

Chaudhary, Zanib; Khan, Gul Majid; Abeer, Muhammad Mustafa; Pujara, Naisarg; Tse, Brian Wan-Chi; McGuckin, Michael A.; Popat, Amirali; Kumeria, Tushar

doi:10.1039/c9bm00822e

Cited by 71 publications

(54 citation statements)

References 65 publications

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“…In the preceding section, we performed absorption measurements at low concentrations to ensure that ICG was present in its monomeric form. However, some imaging experiments, particularly photoacoustic imaging studies [18–20] are performed at high concentrations (∼1 mM). Thus, it is useful to understand the photophysical behavior of ICG at higher concentrations, specifically, the roles of aggregation and self‐quenching.…”

Section: Resultsmentioning

confidence: 99%

“…ICG has been FDA approved for ∼60 years and is used pre‐clinically and clinically in NIR optical imaging applications, including for angiography, [2] lymphatic, [3–6] biliary [7,8] and intestinal [9] functional imaging, dental imaging, [10] and oncological image guided surgery [11–13] . Additionally, there is increasing interest in broader applications of fluorescence guided surgery [14–17] as well as photoacoustic imaging [18–20] with ICG and ICG conjugates. In 2018, it was reported that ICG has a long wavelength emission tail which extends past the NIR region [21,22] .…”

Section: Introductionmentioning

confidence: 99%

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Photophysical Properties of Indocyanine Green in the Shortwave Infrared Region

2021

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With the growing development of new contrast agents for optical imaging using near‐infrared and shortwave infrared (SWIR) wavelengths, it is essential to have consistent benchmarks for emitters in these regions. Indocyanine green (ICG), a ubiquitous and FDA‐approved organic dye and optical imaging agent, is commonly employed as a standard for photophysical properties and biological performance for imaging experiments at these wavelengths. Yet, its reported photophysical properties across organic and aqueous solvents vary greatly in the literature, which hinders its ability to be used as a consistent benchmark. Herein, we measure photophysical properties in organic and aqueous solvents using InGaAs detection (∼950–1,700 nm), providing particular relevance for SWIR imaging.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photophysical Properties of Indocyanine Green in the Shortwave Infrared Region

2021

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“…It has a wide range of applications in the biomedical field and recently has been used to follow the trafficking of nanoscale materials inside cells. The encapsulation of PA imaging agents inside functionalized MSNs has been studied to obtain the most efficient PA imaging performance [ 162 , 163 ]. Thus, there is a potential to use PA methodology in the future to study drug release in vivo relying on its co-delivery with PA imaging molecules.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Mesoporous Silica Particles as Drug Delivery Systems—The State of the Art in Loading Methods and the Recent Progress in Analytical Techniques for Monitoring These Processes

et al. 2021

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Conventional administration of drugs is limited by poor water solubility, low permeability, and mediocre targeting. Safe and effective delivery of drugs and therapeutic agents remains a challenge, especially for complex therapies, such as cancer treatment, pain management, heart failure medication, among several others. Thus, delivery systems designed to improve the pharmacokinetics of loaded molecules, and allowing controlled release and target specific delivery, have received considerable attention in recent years. The last two decades have seen a growing interest among scientists and the pharmaceutical industry in mesoporous silica nanoparticles (MSNs) as drug delivery systems (DDS). This interest is due to the unique physicochemical properties, including high loading capacity, excellent biocompatibility, and easy functionalization. In this review, we discuss the current state of the art related to the preparation of drug-loaded MSNs and their analysis, focusing on the newest advancements, and highlighting the advantages and disadvantages of different methods. Finally, we provide a concise outlook for the remaining challenges in the field.

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“…As a light absorber, ICG is a near-infrared dye that is nearly harmless to the human body and easily metabolized. 26 , 27 , 45 After loading the ICG package into NPs, they are excited by PA instruments to enable PA imaging, which is the basis for the ability of this nanoparticle to act as a molecular probe for PA imaging. Through experiments, we found that in in vitro PA imaging, NPs were able to produce PA signals at low ICG concentrations, and the resulting PA signals could be linearly enhanced as ICG concentrations increased, suggesting that the NPs were able to absorb light and to convert into PA signals.…”

Section: Resultsmentioning

confidence: 99%

iRGD Peptide-Mediated Liposomal Nanoparticles with Photoacoustic/Ultrasound Dual-Modality Imaging for Precision Theranostics Against Hepatocellular Carcinoma

Shi

et al. 2021

IJN

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Purpose Prepare a multifunctional ultrasound molecular probe, cell-penetrating peptide-modified 10-hydroxycamptothecin-loaded phase-transformation lipid nanoparticles (iRGD-ICG-10-HCPT-PFP-NPs), and to combine iRGD-ICG-10-HCPT-PFP -NPs with low-intensity focused ultrasound (LIFU) for precision theranostics against hepatocellular carcinoma (HCC). Materials and Methods The morphology of nanoparticles (NPs) and iRGD-ICG-10-HCPT-PFP-NPs was detected. In vitro, we examined targeting ability by flow cytometry and confocal laser scanning microscopy (CLSM), assessed penetration ability into hepatoma cells, and assessed killing ability. In vivo, we examined the targeting ability of the NPs with a photoacoustic (PA) imager and fluorometer (FL), while LIFU irradiation was used to trigger the release of chemotherapeutic drugs, which had a therapeutic effect on tumors. Results The particle size of iRGD-ICG-10-HCPT-PFP-NPs was 298.4 ± 10.42 nm. In vitro, iRGD-ICG-10-HCPT-PFP-NPs bound more to SK-Hep1 cells than ICG-10-HCPT-PFP-NPs. iRGD-ICG-10-HCPT-PFP-NPs could achieve PA/ultrasound imaging. The percentage of antiproliferative and apoptotic cells in the iRGD-ICG-10-HCPT-PFP-NPs+LIFU group was significantly higher. In vivo, iRGD-ICG-10-HCPT-PFP-NPs can target tumor sites and achieve PA/ultrasound imaging. The tumor volume in the iRGD-ICG-10-HCPT-PFP-NPs+LIFU group was significantly smaller, and the antiproliferative and proapoptotic effects were higher. Conclusion We successfully prepared a novel molecular probe that has good targeting, can perform ultrasound/PA dual-modality imaging, and can penetrate deep into tumors to achieve better therapeutic tumor effects, providing a new idea and method for theranostics of HCC.

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Efficient photoacoustic imaging using indocyanine green (ICG) loaded functionalized mesoporous silica nanoparticles

Abstract: Herein, we demonstrate the role of surface modification of nanocarriers on their ability to load and protect sensitive payloads.

Cited by 71 publications

References 65 publications

Photophysical Properties of Indocyanine Green in the Shortwave Infrared Region

Photophysical Properties of Indocyanine Green in the Shortwave Infrared Region

Mesoporous Silica Particles as Drug Delivery Systems—The State of the Art in Loading Methods and the Recent Progress in Analytical Techniques for Monitoring These Processes

iRGD Peptide-Mediated Liposomal Nanoparticles with Photoacoustic/Ultrasound Dual-Modality Imaging for Precision Theranostics Against Hepatocellular Carcinoma

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