Efficient Polyhydroxyalkanoate Recovery from Recombinant<i>Cupriavidus necator</i>by Using Low Concentration of NaOH

Mohammadi, Mitra; Hassan, Mohd Ali; Phang, Lai Yee; Shirai, Yoshihito; Man, Hasfalina Che; Ariffin, Hidayah; Amirul, A. A.; Syairah, Siti Nor

doi:10.1089/ees.2011.0255

Cited by 20 publications

(19 citation statements)

References 18 publications

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“…It has been found that improvement of the cell wall permeability followed by nonpolymer cellular material (NPCM) release from the cell was the mode of action of NaOH for PHA extraction (Mohammadi et al, 2011a(Mohammadi et al, , 2011b. However, breakage of the cell wall as a result of osmotic pressure throughout the water method caused the release of the intracellular material (Mohammadi et al, 2011c).…”

Section: Effect Of Pha Recovery Methodsmentioning

confidence: 99%

“…EB172 were carried out according to the method as reported by Mumtaz et al (2009) while recombinant C. necator containing PHA was kindly provided by Dr. Kumar Sudesh (Universiti Sains Malaysia, Penang, Malaysia). The preparation of biomass containing PHA prior to recovery process was described elsewhere (Mohammadi et al, 2011a(Mohammadi et al, , 2011b.…”

Section: Pha-containing Biomassmentioning

confidence: 99%

“…PHA purity, recovery yield, protein concentration and polymer molecular weight were analyzed using the protocols as reported previously (Mohammadi et al, 2011a(Mohammadi et al, , 2011b. The morphology of PHA granules before and after the recovery was observed under scanning electron microscopy (JEOL JSM-6400 SEM).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…EB172 and recombinant C. necator using mild alkaline and water recovery methods as cleaner and environmentally more benign process based on several process parameters, i.e. NaOH concentration, time and temperature (Mohammadi et al, 2011a(Mohammadi et al, , 2011b(Mohammadi et al, , 2011c. However, the halogen-free methods chosen in this study require further adjustment, depending on both significant process parameters and external factors influencing their performance to make the process suitable for polymer recovery at the industrial scale.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Mohammadi

Hassan

Phang

et al. 2012

Journal of Cleaner Production

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Section: Effect Of Pha Recovery Methodsmentioning

confidence: 99%

Section: Pha-containing Biomassmentioning

confidence: 99%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Mohammadi

Hassan

Phang

et al. 2012

Journal of Cleaner Production

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“…EB172 [10][11][12][13][14]. While developing a suitable fermentation strategy to feed mixed acids in its original form (keeping identical ratio as obtained), our focus was also to develop a non-halogenated PHA recovery system, at the end of fed-batch fermentation [10,15,16]. However, Comamonas sp.…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172

Yee¹,

Mumtaz²,

Mohammadi³

et al. 2012

J Microb Biochem Technol

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Recombinant Escherichia coli JM109 harbouring the polyhydroxyalkanoate (PHA) biosynthesis gene (phaCABco) of Comamonas sp. EB172, an acid tolerant microbe, was examined for the production of PHAs from various carbon sources. The study demonstrated that the recombinant E. coli JM109 had the potential to utilize both sugar-and acid-based carbon sources, for the biosynthesis of both poly(3-hydroxybutyrate) P(3HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymers. In the shake flask experiments, the strain was capable of producing P (3HB-co-3HV) copolymer from mixed organic acids, and higher productivities were obtained using glucose compared to mixed acids. However, PHA accumulation was found to be similar, regardless of the carbon source used. Nitrogen supplementation in the medium was found to improve the cell dry weight, but negatively affected the 3HV formation in copolymer production. Maximum 3HV monomer (3 mol%) was obtained with C/N 42.1, using mixed acids as the carbon source. In the 2L bioreactor, the productivity and yield based on substrate utilization coefficient were found to be 0.16 g PHA/(L.h) and 0.41 g PHA/g substrate under C/N around 75, using 20 g/L glucose and 0.5 g/L ammonium sulphate, respectively. The polymer produced by the recombinant strain had molecular weight in the range of 8.5 x 105 to 1.4 x 106 Da. Overall, the ability of the recombinant E. coli JM109 to utilize both glucose and mixed acids, has widened its substrate selection for fermentation, including the opportunity to use renewable biomass.

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Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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Efficient Polyhydroxyalkanoate Recovery from RecombinantCupriavidus necatorby Using Low Concentration of NaOH

Cited by 20 publications

References 18 publications

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Polyhydroxyalkanoate Synthesis by Recombinant Escherichia coli JM109 Expressing PHA Biosynthesis Genes from Comamonas sp. EB172

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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