2024
DOI: 10.3390/fermentation10040208
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Efficient Secretory Expression for Mammalian Hemoglobins in Pichia pastoris

Chenyang Li,
Tao Zhang,
Zhengshan Luo
et al.

Abstract: Mammalian hemoglobins (HB) are a kind of heme-binding proteins that play crucial physiological roles in various organisms. The traditional techniques employed for the extraction of HB are expensive and time-consuming, while the yields of mammalian HB in previous reports were quite low. The industrial Pichia pastoris is a highly effective platform for the secretory expression of heterologous proteins. To achieve efficient secretory expression of HB in P. pastoris, multiple strategies were applied, including the… Show more

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